| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,040,661 | G→A | 17.6% | intergenic (+25/+245) | rrfA → / ← mobB | 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,040,661 | 0 | G | A | 17.6% | 112.4 / 19.8 | 51 | intergenic (+25/+245) | rrfA/mobB | 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B |
| Reads supporting (aligned to +/- strand): ref base G (18/24); new base A (7/2); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01 | |||||||||||
TTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCA > NC_000913/4040515‑4040803 | tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 2:874761/149‑1 (MQ=34)tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 2:287023/149‑1 (MQ=34)tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 2:140314/149‑1 (MQ=34)tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 1:992948/149‑1 (MQ=34)tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 3:260521/149‑1 (MQ=35) gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGAc > 2:998382/1‑149 (MQ=34) gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGAc > 4:717256/1‑149 (MQ=34) gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGAc < 3:193956/149‑1 (MQ=35) cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTATGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGAc < 4:1105974/147‑1 (MQ=25) cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTATGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGAc > 3:1105974/1‑147 (MQ=25) cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTg < 1:998382/149‑1 (MQ=34) tGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTgg < 2:899928/149‑1 (MQ=35) ggcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTggg < 1:1214352/149‑1 (MQ=34) ggcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTggg < 3:1314293/149‑1 (MQ=35) gcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCtt > 1:1098407/1‑149 (MQ=35) aGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTc < 2:1098407/149‑1 (MQ=35) gtggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCt < 3:717256/149‑1 (MQ=34) tGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGCCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGa < 3:1699535/107‑1 (MQ=25) tGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGCCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGa > 4:1699535/1‑107 (MQ=25) gAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa < 2:1538538/97‑1 (MQ=37) gAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGaa > 1:1538538/1‑97 (MQ=37) gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc > 3:768631/1‑111 (MQ=32) gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc < 4:768631/111‑1 (MQ=33) gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCgg > 1:887954/1‑128 (MQ=32) gAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCgg < 2:887954/128‑1 (MQ=33) aaaGGCTCAGTCGGAAGACTGGGCCTTTTGTTTTATctgttgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactg > 4:786340‑M1/1‑36 (MQ=255) aaaGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatagggtcaggtg < 4:397701‑M1/132‑97 (MQ=255) aaaGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatagggtcaggtg > 3:397701‑M1/1‑36 (MQ=255) aaGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgc > 2:300317‑M1/1‑35 (MQ=255) aGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgag < 1:243271‑M1/67‑34 (MQ=255) aGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgag > 2:243271‑M1/1‑34 (MQ=255) aGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgcc > 2:1147412‑M1/1‑34 (MQ=255) ggCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttct < 2:653576‑M1/107‑75 (MQ=255) ggCTCAGTCGGAAGACTGGGCCTTTTGTTTTATttgatgtctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttct > 1:653576‑M1/1‑33 (MQ=255) ggCTCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccg > 4:476783‑M1/1‑33 (MQ=255) gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATctgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgc > 2:541696‑M1/1‑32 (MQ=255) gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATctgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgc > 2:450745‑M1/1‑32 (MQ=255) gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAg > 4:1138682/1‑115 (MQ=32) gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAg < 3:1138682/115‑1 (MQ=32) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgc > 1:1016417‑M1/1‑32 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgc > 2:1417042‑M1/1‑32 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgc < 1:675341‑M1/146‑115 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgc > 2:675341‑M1/1‑32 (MQ=255) gCTCAGTCGAAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatggggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcacgtgggaccaccgcgctactgccgc > 4:45740‑M1/1‑32 (MQ=255) tCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctga > 4:1400310‑M1/1‑30 (MQ=255) tCAGTCGGAAGACTGGGCCTTTCGTTTTATttgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctga < 3:1400310‑M1/106‑77 (MQ=255) cAGTCGGAAGACTGGGCCTTTCGTTTTATctgatgcctggcagttccctactctcgcatgaggagaccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccag < 1:541696‑M1/149‑121 (MQ=255) tCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa < 2:235182/55‑1 (MQ=38) tCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGa > 1:235182/1‑55 (MQ=35) tCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGc < 2:1132479/97‑1 (MQ=34) tCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGc > 1:1132479/1‑97 (MQ=33) ggAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg > 4:1551484/1‑109 (MQ=31) ggAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg < 3:1551484/109‑1 (MQ=31) | TTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCA > NC_000913/4040515‑4040803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |