| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,196,354 | G→C | 6.0% | A152G (GCC→GGC) | rsd ← | stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,354 | 0 | G | C | 6.0% | 667.6 / 22.1 | 183 | A152G (GCC→GGC) | rsd | stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base G (81/91); new base C (3/8); total (84/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 191 total aligned reads displayed.
AGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAA > NC_000913/4196206‑4196501 | aGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcggg > 2:1393547/1‑149 (MQ=255) aTTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGggcggc < 3:861525/149‑1 (MQ=255) ttAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGgcggcg > 4:1713426/1‑149 (MQ=255) aGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTc < 4:1875634/149‑1 (MQ=255) ccTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCaa > 2:1479031/1‑149 (MQ=255) cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 4:1409420/149‑1 (MQ=255) cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCCGCCCGCGTCaaa < 2:1285517/149‑2 (MQ=255) cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCCGCCCGCGTCaaa < 1:516020/149‑2 (MQ=255) tGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGc < 2:263870/149‑1 (MQ=255) tCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCTGAACTCAATCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc > 4:1137995/1‑149 (MQ=255) tCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGCTTGACGCCGCCCGCGTCaaacatc < 3:1299976/149‑6 (MQ=255) cAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACca > 1:949003/1‑149 (MQ=255) gTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca < 2:784268/149‑1 (MQ=255) cgcgGATCCGGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAAt < 3:1596624/149‑1 (MQ=255) gcgGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATc > 2:514746/1‑149 (MQ=255) gATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGc < 3:1085356/149‑1 (MQ=255) gATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGc < 1:1393547/149‑1 (MQ=255) tCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCtt > 1:1871843/1‑149 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTc > 3:122097/1‑124 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTc < 3:923393/124‑1 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTc < 4:122097/124‑1 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTc > 4:923393/1‑124 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCaa > 2:1215366/1‑126 (MQ=255) aaTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCtt > 3:1483187/1‑149 (MQ=255) aTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTc < 1:31796/149‑1 (MQ=255) tAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCtt < 1:1801489/147‑1 (MQ=255) tAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTcc < 2:319133/149‑1 (MQ=255) aaaCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCa < 1:779854/149‑1 (MQ=255) ctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACaaa > 3:1505969/1‑149 (MQ=255) cAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAAGGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcgc < 2:1765603/149‑1 (MQ=255) aGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt < 2:716488/149‑1 (MQ=255) ttAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATc < 1:1019278/128‑1 (MQ=255) ttAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTc < 4:1720822/149‑1 (MQ=255) ttAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTc > 2:1035830/1‑149 (MQ=255) tCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTc > 2:136883/1‑149 (MQ=255) gACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGcc < 1:1114847/149‑1 (MQ=255) cAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGa > 1:1084180/1‑149 (MQ=255) aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGAt > 3:721242/1‑149 (MQ=255) aGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATg > 1:555987/1‑149 (MQ=255) gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTcc < 4:333817/115‑1 (MQ=255) gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTcc > 3:333817/1‑115 (MQ=255) gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGt < 1:136883/149‑1 (MQ=255) aaaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTc < 1:510331/149‑1 (MQ=255) aaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCa < 4:1483187/149‑1 (MQ=255) ttAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCCCTTCCAGCGCCTCGCCGATGTCAGa > 3:1535362/1‑149 (MQ=255) tAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGAt < 4:1760433/149‑1 (MQ=255) ccccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATaaa > 4:603483/1‑149 (MQ=255) ccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAAc < 4:1589914/149‑1 (MQ=255) gCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCt < 3:782116/149‑1 (MQ=255) cAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTg < 1:1035830/149‑1 (MQ=255) aaTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGtt < 2:1084180/149‑1 (MQ=255) gggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGaaa > 4:42474/1‑149 (MQ=255) ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAAc < 1:1479031/149‑1 (MQ=255) cATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTc < 2:949003/149‑1 (MQ=255) ttGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATg > 3:501086/1‑124 (MQ=255) ttGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATg < 4:501086/124‑1 (MQ=255) aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGt > 2:1585220/1‑149 (MQ=255) aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGt > 3:1666076/1‑149 (MQ=255) tGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTaa > 2:650066/1‑149 (MQ=255) tAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCa < 2:593160/120‑1 (MQ=255) tAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCa > 1:593160/1‑120 (MQ=255) aaaTTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTa > 4:1678910/1‑149 (MQ=255) ttACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCTTCGCCGATGTCAGATAAAACCTGTTGaaa < 1:1491644/133‑1 (MQ=255) tACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCAt < 2:1488645/149‑1 (MQ=255) gcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGaaa > 4:1024187/1‑129 (MQ=255) gcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCAt > 2:1817926/1‑146 (MQ=255) gcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGAt > 4:1293911/1‑149 (MQ=255) gTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCa > 4:8827/1‑131 (MQ=255) gTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCa < 3:8827/131‑1 (MQ=255) gTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCg > 2:991487/1‑149 (MQ=255) tAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGAt < 4:1666076/149‑1 (MQ=255) cAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGc < 1:650066/149‑1 (MQ=255) cAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGc < 2:5563/149‑1 (MQ=255) aGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGt < 3:1753791/116‑1 (MQ=255) cgcAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGt < 1:716448/149‑1 (MQ=255) aGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTc < 1:1279828/149‑1 (MQ=255) gAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTcc < 3:1678910/149‑1 (MQ=255) tCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCAt < 1:1574614/128‑1 (MQ=255) tCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGAt > 2:156002/1‑135 (MQ=255) cAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACt < 2:1578904/149‑1 (MQ=255) aaGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAAc < 1:1554965/110‑1 (MQ=255) aaGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTg > 4:72931/1‑149 (MQ=255) aaGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTg > 2:712773/1‑149 (MQ=255) aaGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTg < 1:1585220/149‑1 (MQ=255) aGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCa < 1:158099/124‑1 (MQ=255) gCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGtt < 4:1533118/103‑1 (MQ=255) gCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGAt < 1:610906/127‑1 (MQ=255) gCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGAt > 2:610906/1‑127 (MQ=255) ggATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATc < 3:1186610/149‑1 (MQ=255) ggATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATc < 3:1713426/149‑1 (MQ=255) gATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGa < 3:1043196/88‑1 (MQ=255) gATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGa > 4:1043196/1‑88 (MQ=255) tGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGTCTGGAATCgta < 1:991487/149‑1 (MQ=255) tGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgta < 1:712773/149‑1 (MQ=255) tGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgta < 3:42474/149‑1 (MQ=255) ttGACGCCGCCCGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAgcgc < 3:600668/68‑1 (MQ=38) ttGACGCCGCCCGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTc < 4:846464/131‑1 (MQ=255) gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAAt > 3:1772196/1‑149 (MQ=255) cgcgGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATcc < 2:1541447/149‑1 (MQ=255) cgcgGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATcc < 3:18936/149‑1 (MQ=255) | AGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAA > NC_000913/4196206‑4196501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |