Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,218,447 A→G 8.1% intergenic (+34/‑149) aceA → / → aceK isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,218,4470AG 8.1% 484.3 / 20.1 147intergenic (+34/‑149)aceA/aceKisocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base A (67/68);  new base G (5/7);  total (72/75)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGC  >  NC_000913/4218303‑4218592
                                                                                                                                                |                                                                                                                                                 
cACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATaa                                                                                                                                                  <  2:368401/146‑1 (MQ=255)
 aCCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCg                                                                                                                                              <  4:1806971/149‑1 (MQ=255)
  ccAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGt                                                                                                                                             <  2:233817/149‑1 (MQ=255)
     gcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa                                                                                                                                          <  3:1420397/149‑1 (MQ=255)
            gTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAg                                                                                                                                                 >  2:652327/1‑135 (MQ=255)
            gTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAg                                                                                                                                                 <  1:652327/135‑1 (MQ=255)
             tGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                                                  >  3:1392762/1‑149 (MQ=255)
              gggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                                 <  1:385868/149‑1 (MQ=255)
               ggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                >  2:782475/1‑149 (MQ=255)
                        tACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCgg                                                                                                                       >  2:246374/1‑149 (MQ=255)
                           ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                                                                                 >  1:1621096/1‑131 (MQ=255)
                           ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                             <  2:1640816/140‑1 (MQ=255)
                           ttCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGc                                                                                                                    >  3:1533621/1‑149 (MQ=255)
                              gATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                                                                                 <  3:758931/133‑6 (MQ=255)
                                  aaGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGc                                                                                                             >  4:1544077/1‑149 (MQ=255)
                                    gTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                               >  3:839789/1‑129 (MQ=255)
                                        cgacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAg                                                                                                                                                 >  4:1429451/1‑107 (MQ=255)
                                        cgacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAg                                                                                                                                                 <  3:1429451/107‑1 (MQ=255)
                                         gacTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                                                                           <  2:588688/144‑1 (MQ=255)
                                            tattatTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGAc                                                                                                   >  4:720722/1‑149 (MQ=255)
                                             attattCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACg                                                                                                  >  4:543708/1‑149 (MQ=255)
                                              ttattCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGc                                                                                                 >  4:802980/1‑149 (MQ=255)
                                                 ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                             >  1:39343/1‑118 (MQ=38)
                                                 ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGa                                                                                                         <  4:967186/138‑1 (MQ=255)
                                                 ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGa                                                                                                         >  3:967186/1‑138 (MQ=255)
                                                 ttCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTg                                                                                              >  4:850948/1‑149 (MQ=255)
                                                    aGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGc                                                                                                      <  1:642236/138‑1 (MQ=255)
                                                    aGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTgcgc                                                                                           >  4:1872684/1‑149 (MQ=255)
                                                      ggcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGt                                                                                         <  2:679219/149‑1 (MQ=255)
                                                       gcggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTc                                                                                        <  3:413323/149‑1 (MQ=255)
                                                        cggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCt                                                                                       <  3:1812940/149‑1 (MQ=255)
                                                        cggcACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCt                                                                                       <  3:850948/149‑1 (MQ=255)
                                                            aCGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                    >  1:1764553/1‑100 (MQ=255)
                                                            aCGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGcc                                                                                                            >  4:1858340/1‑124 (MQ=255)
                                                              gTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCAt                                                                                 >  2:1529899/1‑149 (MQ=255)
                                                              gTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCAt                                                                                 >  4:58855/1‑149 (MQ=255)
                                                               tCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATg                                                                                >  3:1508146/1‑149 (MQ=255)
                                                                 ttCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGcc                                                                              >  2:1782287/1‑149 (MQ=255)
                                                                   cAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGc                                                                                                                                               <  4:1243369/82‑1 (MQ=255)
                                                                      tCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAg                                                                         >  3:1531869/1‑149 (MQ=255)
                                                                        aCCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGcc                                                                       >  1:192222/1‑149 (MQ=255)
                                                                        aCCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGcc                                                                       >  1:720311/1‑149 (MQ=255)
                                                                             gcTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                                  <  2:192222/149‑1 (MQ=255)
                                                                                   cGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                                 <  4:180228/80‑1 (MQ=255)
                                                                                     gCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTa                                                          <  3:67425/149‑1 (MQ=255)
                                                                                      cTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAg                                                         <  4:1531869/149‑1 (MQ=255)
                                                                                       tCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                                                                           <  3:228928/98‑1 (MQ=255)
                                                                                       tCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                                  >  1:294870/1‑139 (MQ=255)
                                                                                          aCTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTg                                                     <  3:1870406/149‑1 (MQ=255)
                                                                                            tGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTAt                                                                                    >  3:1098991/1‑116 (MQ=255)
                                                                                            tGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTAt                                                                                    <  2:421705/116‑1 (MQ=255)
                                                                                            tGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTa                                                          >  1:558816/1‑142 (MQ=255)
                                                                                            tGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa                                                   >  4:9229/1‑149 (MQ=255)
                                                                                            tGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa                                                   <  1:1127983/149‑1 (MQ=255)
                                                                                             gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt                                                  <  3:58855/149‑1 (MQ=255)
                                                                                             gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt                                                  <  1:1529899/149‑1 (MQ=255)
                                                                                               agaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATaa                                                <  3:1614305/149‑1 (MQ=255)
                                                                                               agaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATaa                                                >  4:745016/1‑149 (MQ=255)
                                                                                                gaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAg                                               >  3:1855044/1‑149 (MQ=255)
                                                                                                gaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAg                                               <  3:1872684/149‑1 (MQ=255)
                                                                                                 aaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAgg                                              <  1:198142/149‑1 (MQ=255)
                                                                                                   tCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGt                                                                                         <  3:1585208/104‑1 (MQ=255)
                                                                                                   tCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGt                                                                                         >  4:1585208/1‑104 (MQ=255)
                                                                                                     gCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGtt                                          <  1:782475/149‑1 (MQ=255)
                                                                                                       aGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTa                                        <  4:1855044/149‑1 (MQ=255)
                                                                                                              aGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                                                                           >  1:549442/1‑75 (MQ=255)
                                                                                                              aGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCt                                                                                                           <  2:549442/75‑1 (MQ=255)
                                                                                                              aGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCAt                                                                                 <  1:1488965/101‑1 (MQ=255)
                                                                                                              aGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCAt                                                                                 <  3:1782902/101‑1 (MQ=255)
                                                                                                               gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCAt                                >  2:1494423/1‑149 (MQ=255)
                                                                                                                 aaccacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGt                                                                                                                      <  4:1395179/61‑1 (MQ=255)
                                                                                                                 aacaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATcc                              >  3:1157054/1‑149 (MQ=255)
                                                                                                                     acaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCa                          >  3:232532/1‑149 (MQ=255)
                                                                                                                      caacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAAc                                                             <  1:121618/113‑1 (MQ=255)
                                                                                                                      caacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAAc                                                             >  2:121618/1‑113 (MQ=255)
                                                                                                                      caacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTg                                                     >  4:1709982/1‑121 (MQ=255)
                                                                                                                       aacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGc                                             >  2:876409/1‑128 (MQ=255)
                                                                                                                       aacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAt                        >  2:1202097/1‑149 (MQ=255)
                                                                                                                        acCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                                  >  4:1277410/1‑106 (MQ=255)
                                                                                                                        acCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGc                                                                  <  3:1277410/106‑1 (MQ=255)
                                                                                                                         ccGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtc                      >  4:324187/1‑149 (MQ=255)
                                                                                                                         ccGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtc                      >  3:1751754/1‑149 (MQ=255)
                                                                                                                         ccGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtc                      >  2:1721215/1‑149 (MQ=255)
                                                                                                                          cGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtct                     <  3:324187/149‑1 (MQ=255)
                                                                                                                          cGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtct                     <  1:1721215/149‑1 (MQ=255)
                                                                                                                           gTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtctc                    <  4:1751754/149‑1 (MQ=255)
                                                                                                                           gTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtctc                    <  4:232532/149‑1 (MQ=255)
                                                                                                                           gTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtctc                    <  4:1508146/149‑1 (MQ=255)
                                                                                                                             tGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTg                  <  3:994353/149‑1 (MQ=255)
                                                                                                                                tGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTAc                                       >  3:34318/1‑125 (MQ=255)
                                                                                                                                tGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTc               >  3:473830/1‑149 (MQ=255)
                                                                                                                                    tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtga           <  1:1202097/149‑1 (MQ=255)
                                                                                                                                    tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtga           <  4:207401/149‑1 (MQ=255)
                                                                                                                                       aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtgatga        <  3:745016/149‑1 (MQ=255)
                                                                                                                                       aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtgatga        >  2:581768/1‑149 (MQ=255)
                                                                                                                                        ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAg       <  1:1494423/149‑1 (MQ=255)
                                                                                                                                        ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGaa                                                              >  1:337822/1‑94 (MQ=255)
                                                                                                                                        ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGaa                                                              <  2:337822/94‑1 (MQ=255)
                                                                                                                                             gATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGgcgc  >  1:945935/1‑149 (MQ=255)
                                                                                                                                               tAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTcc              >  1:928301/1‑135 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGATGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGATGAGGGCGC  >  NC_000913/4218303‑4218592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: