| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,223,855 | G→A | 5.2% | A10T (GCG→ACG) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,223,855 | 0 | G | A | 5.2% | 913.8 / 26.1 | 267 | A10T (GCG→ACG) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (131/122); new base A (7/7); total (138/129) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
Only 100 of 280 total aligned reads displayed.
GATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA > NC_000913/4223707‑4224002 | gTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg > 1:194779/1‑149 (MQ=255) tAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt < 4:1769311/149‑1 (MQ=255) tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCa > 4:70768/1‑143 (MQ=255) tGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCa < 3:70768/143‑1 (MQ=255) ttGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATg > 3:651129/1‑149 (MQ=255) tGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGa < 3:1271918/149‑1 (MQ=255) aCAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg > 2:229493/1‑149 (MQ=255) cAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt > 3:61140/1‑149 (MQ=255) aaaTCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa < 2:596712/145‑1 (MQ=255) aaaTCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTa < 4:277900/149‑1 (MQ=255) aaaTCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTa > 1:1733430/1‑149 (MQ=255) tctcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc > 1:340318/1‑149 (MQ=255) tcATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg < 1:829833/149‑1 (MQ=255) gtCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt < 3:223760/117‑1 (MQ=255) gtCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGt > 1:762057/1‑149 (MQ=255) tCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGt > 2:1592797/1‑148 (MQ=255) tCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTa > 2:531044/1‑149 (MQ=255) tCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGCACGCAGttgtt > 1:1094281/1‑117 (MQ=255) ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCATTTAAATGAACg < 3:1085455/122‑1 (MQ=255) ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa > 4:1042820/1‑116 (MQ=255) ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa < 3:1042820/116‑1 (MQ=255) ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACg > 4:1085455/1‑122 (MQ=255) ttttACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc > 2:1735612/1‑127 (MQ=255) tACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCAc < 2:762057/149‑1 (MQ=255) cacaGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCAt > 1:437337/1‑149 (MQ=255) gCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCag < 2:131859/149‑1 (MQ=255) cGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga < 4:846074/149‑1 (MQ=255) gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa > 4:290338/1‑101 (MQ=255) gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa < 3:290338/101‑1 (MQ=255) gTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga < 2:1232401/148‑1 (MQ=255) gCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt < 3:844541/92‑1 (MQ=255) ccAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCa > 2:730742/1‑129 (MQ=255) gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAAc > 1:1763206/1‑91 (MQ=255) gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg < 3:1861605/99‑1 (MQ=255) gTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGa > 4:1706688/1‑149 (MQ=255) ttgttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAg < 3:746304/149‑1 (MQ=255) ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGTGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCg > 4:357300/1‑149 (MQ=255) ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGaa < 4:91676/145‑1 (MQ=255) ttgAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCg < 2:1779430/149‑1 (MQ=255) aTTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAttt > 2:336300/1‑149 (MQ=255) tttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtttt > 1:1840538/1‑149 (MQ=255) tttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtttt > 4:1342585/1‑149 (MQ=255) ttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTc < 2:1871352/149‑1 (MQ=255) ttttATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTc > 3:935935/1‑149 (MQ=255) tattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg < 1:542149/149‑1 (MQ=255) tattaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtg < 2:1020599/149‑1 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt > 1:908903/1‑88 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga > 2:369553/1‑117 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggt > 1:1592470/1‑149 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggt < 3:133815/149‑1 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggt > 3:1189980/1‑149 (MQ=255) ttaAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgtggt > 3:1792453/1‑149 (MQ=255) aTCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAAc > 4:1805979/1‑149 (MQ=255) tCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACg > 1:1823488/1‑149 (MQ=255) cTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGc < 4:189744/149‑1 (MQ=255) ggTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGTGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCCTGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCttt < 4:1269800/149‑1 (MQ=255) ggTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTAt < 1:39320/91‑1 (MQ=255) gTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTg < 3:1366800/149‑1 (MQ=255) gTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTg < 2:1576834/149‑1 (MQ=255) gtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg < 4:446920/64‑1 (MQ=255) gtCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg > 3:446920/1‑64 (MQ=255) gggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGa > 1:700501/1‑103 (MQ=255) gggAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCAt > 4:24743/1‑149 (MQ=255) gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt < 2:1822633/122‑1 (MQ=255) gAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGc < 1:264786/149‑1 (MQ=255) aGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCg > 3:318498/1‑149 (MQ=255) cAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGAc > 3:1558609/1‑149 (MQ=255) cAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGAc < 3:887927/149‑1 (MQ=255) gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGAtt < 1:1803129/111‑1 (MQ=255) gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCgt > 2:40656/1‑116 (MQ=255) gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGc > 3:1684708/1‑143 (MQ=255) gtgtGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGc < 4:1684708/143‑1 (MQ=255) gtgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATTGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaa < 2:855626/149‑1 (MQ=255) gtgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCaga > 4:78183/1‑85 (MQ=255) tgAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa < 4:1189980/149‑1 (MQ=255) gAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg < 3:1240133/149‑1 (MQ=255) gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc < 3:357300/149‑1 (MQ=255) gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc < 2:1122208/149‑1 (MQ=255) gcagcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGc < 3:1706688/149‑1 (MQ=255) tgcaAAGTGGAACTACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCTGAGTTATCGACTGAGCGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaa > 4:1637482/2‑149 (MQ=255) gcaAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCag < 2:1824683/77‑1 (MQ=255) aaGTGGAACAACTGCGTGCGCAGTTAAATGCACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGc < 2:292783/126‑1 (MQ=255) aaGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCaacaac > 4:1368841/1‑149 (MQ=255) aGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACg > 1:1632841/1‑149 (MQ=255) tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCaaa < 1:1285562/137‑1 (MQ=255) tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAc < 1:102442/149‑1 (MQ=255) tGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAc < 1:1213706/149‑1 (MQ=255) ggAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAg < 2:1249332/137‑1 (MQ=255) ggAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAcc > 1:1427879/1‑149 (MQ=255) ggAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAcc < 1:734467/149‑1 (MQ=255) gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct > 4:1508662/1‑149 (MQ=255) gAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct > 2:1072767/1‑149 (MQ=255) tttaacTGCGCACGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctg > 1:1247513/4‑149 (MQ=255) aacaacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCttt < 4:658246/110‑1 (MQ=255) caacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCAc > 2:950304/1‑56 (MQ=255) aacTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg > 2:1638781/1‑149 (MQ=255) acTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTgg < 4:123161/149‑1 (MQ=255) tgcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGAcgg < 4:633312/40‑1 (MQ=255) tgcgtgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTa > 2:704590/1‑149 (MQ=255) tgcgCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCa < 3:263821/149‑1 (MQ=255) | GATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA > NC_000913/4223707‑4224002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |