Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,245,113 C→A 6.1% intergenic (‑38/+116) malF ← / ← malE maltose transporter subunit/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,245,1130CA 6.1% 479.4 / 14.8 132intergenic (‑38/+116)malF/malEmaltose transporter subunit/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (60/64);  new base A (4/4);  total (64/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 134 total aligned reads displayed.

TGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTC  >  NC_000913/4244966‑4245255
                                                                                                                                                   |                                                                                                                                              
tgtgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACg                                                                                                                                               <  3:517738/149‑1 (MQ=255)
  tgCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGcc                                                                                                                                             <  2:1858780/149‑1 (MQ=255)
   gCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                            >  4:1701055/1‑149 (MQ=255)
     gTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCCATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTa                                                                                                                                          <  3:1701055/149‑1 (MQ=255)
     gTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTa                                                                                                                                          <  2:1342425/149‑1 (MQ=255)
      tACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                         >  4:1278390/1‑149 (MQ=255)
       aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        >  2:345372/1‑149 (MQ=255)
       aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        <  3:1039644/149‑1 (MQ=255)
       aCATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        <  4:325187/149‑1 (MQ=255)
            aaaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                         >  4:926003/1‑143 (MQ=255)
            aaaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                         <  3:926003/143‑1 (MQ=255)
             aaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                     >  2:1621720/1‑146 (MQ=255)
             aaaCAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                     <  1:1621720/146‑1 (MQ=255)
                 aacaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                              >  1:1643674/1‑149 (MQ=255)
                 aacaaGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                         <  3:612145/138‑1 (MQ=255)
                 aacaaGGTAACCCACCAGCAGGCCGAGCAGACCAAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACaacaac                                                                                                                              <  1:1606768/149‑1 (MQ=255)
                      ggTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        >  1:1031770/1‑134 (MQ=255)
                      ggTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATc                                                                                                                                        <  2:1031770/134‑1 (MQ=255)
                            ccaccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATcc                                                                                                                                       <  2:1674976/129‑1 (MQ=255)
                            ccaccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATcc                                                                                                                                       >  1:1674976/1‑129 (MQ=255)
                                          aGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                     >  1:304954/1‑149 (MQ=255)
                                              gACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAAAGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATc                                                                                                 <  3:1632264/149‑1 (MQ=255)
                                               aCCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCa                                                                                                <  1:156745/149‑1 (MQ=255)
                                                 cTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCGTGAAAGCCTTAt                                                                                                                                         >  3:609267/1‑106 (MQ=255)
                                                       cTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                            >  4:286978/1‑97 (MQ=255)
                                                       cTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCt                                                                                                                                            <  3:286978/97‑1 (MQ=255)
                                                       cTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACa                                                                                        <  2:1643674/149‑1 (MQ=255)
                                                         gACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATa                                                                                      >  2:1435049/1‑149 (MQ=255)
                                                          aCCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGa                                                                                                            <  2:1281154/126‑1 (MQ=255)
                                                          aCCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGa                                                                                                            >  1:1281154/1‑126 (MQ=255)
                                                            cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGcc                                                                                                                                             <  2:400681/91‑1 (MQ=255)
                                                            cATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGcc                                                                                                                                             >  1:400681/1‑91 (MQ=255)
                                                             aTTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGc                                                                                                                           <  3:1859219/108‑1 (MQ=255)
                                                             aTTTCAGCGCGTCGCTTTACCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATg                                                                                                                     >  3:647257/1‑114 (MQ=255)
                                                             aTTTCAGCGCGTCGCTTTACCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATg                                                                                                                     <  4:647257/114‑1 (MQ=255)
                                                              tttCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGttt                                                                                 >  3:1275069/1‑149 (MQ=255)
                                                                   gcgcGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTACTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtt                                                                            <  4:1698244/149‑1 (MQ=255)
                                                                     gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                          <  1:1435049/149‑1 (MQ=255)
                                                                       gTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCt                                                                                                                         >  3:696576/1‑100 (MQ=255)
                                                                                  accaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGcc                                                                                                                          <  4:288409/88‑1 (MQ=255)
                                                                                  accaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                     >  1:837833/1‑109 (MQ=255)
                                                                                  accaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                     >  3:669269/1‑109 (MQ=255)
                                                                                  accaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCt                                                                                                     <  4:669269/109‑1 (MQ=255)
                                                                                  accaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACaa                                                             >  2:945746/1‑149 (MQ=255)
                                                                                    caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                     <  3:1534329/75‑1 (MQ=255)
                                                                                    caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGa                                                                                                                                     >  4:1534329/1‑75 (MQ=255)
                                                                                    caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                                                                                              >  1:196367/1‑98 (MQ=255)
                                                                                    caATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                                                                                              <  2:196367/98‑1 (MQ=255)
                                                                                         tttCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACa                                                                                        >  3:1081029/1‑115 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                         >  3:935449/1‑62 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTAt                                                                                                                                         <  4:935449/62‑1 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGa                                                                                                                  >  4:640030/1‑85 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                      >  4:1385995/1‑97 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTc                                                                                                      >  2:545405/1‑97 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                          <  4:1107700/125‑1 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGtt                                                       <  2:380351/144‑1 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGtt                                                       >  1:380351/1‑144 (MQ=255)
                                                                                             tttttAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgc                                                  >  3:309406/1‑149 (MQ=255)
                                                                                                  aaTGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGAcaacaa                                                                                                                               <  2:1016552/67‑1 (MQ=255)
                                                                                                  aaTGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGAcaacaa                                                                                                                               >  1:1016552/1‑67 (MQ=255)
                                                                                                  aaTGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                                                                                              >  1:529576/1‑84 (MQ=255)
                                                                                                          ccATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAg                                                                      >  3:1193903/1‑116 (MQ=255)
                                                                                                             tGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTca                                  <  4:125528/149‑1 (MQ=255)
                                                                                                                 gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                                                                         >  2:1554878/1‑90 (MQ=255)
                                                                                                                 gTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTAc                                                                                         <  1:1554878/90‑1 (MQ=255)
                                                                                                                  ttcttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCa                             <  2:304954/149‑1 (MQ=255)
                                                                                                                     ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGttt                                                                                 >  4:506318/1‑94 (MQ=255)
                                                                                                                     ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCg                                                         <  3:1213483/118‑1 (MQ=255)
                                                                                                                     ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCg                                                         >  4:1213483/1‑118 (MQ=255)
                                                                                                                     ttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGt                                                        <  1:332829/119‑1 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGc                                                                                                                    <  2:764712/56‑1 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGc                                                                                                                    >  1:764712/1‑56 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                          >  2:1791177/1‑98 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGtttt                                                                          <  1:1791177/98‑1 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCAt                                             >  4:76325/1‑127 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCAt                                             <  3:76325/127‑1 (MQ=255)
                                                                                                                        cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTAACGCGTCTTATCAGGCCCACATACGTTTCGGTTTTGTAGGCCGGAc                                                               >  3:310099/1‑109 (MQ=255)
                                                                                                                         tCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGc                                                                                                                           <  3:939869/48‑1 (MQ=255)
                                                                                                                           aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgccg                                                >  4:1127589/1‑121 (MQ=255)
                                                                                                                           aTTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCAcgccg                                                <  3:1127589/121‑1 (MQ=255)
                                                                                                                            ttCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGc                                                          <  1:1671650/110‑1 (MQ=255)
                                                                                                                            ttCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   <  3:1278390/149‑1 (MQ=255)
                                                                                                                             tCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGa                  <  2:1661447/149‑1 (MQ=255)
                                                                                                                                  gACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                     <  3:866769/141‑1 (MQ=255)
                                                                                                                                   tcGGATAAGGCGTGAAAGCCTTATCCGTCAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAg            >  3:1186118/2‑149 (MQ=38)
                                                                                                                                    cGGATAAGGCGTGAAAGCCTTATCCGTCAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGt           <  4:1186118/149‑1 (MQ=38)
                                                                                                                                      gATAAGGCGTGAAAGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                     >  2:1725363/1‑137 (MQ=255)
                                                                                                                                       aTAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTc                                                                                >  4:316193/1‑77 (MQ=255)
                                                                                                                                       aTAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTc                                                                                <  3:316193/77‑1 (MQ=255)
                                                                                                                                        tAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTgc       >  4:190659/1‑149 (MQ=255)
                                                                                                                                           ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGt                                                                             <  1:759433/76‑1 (MQ=255)
                                                                                                                                           ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGt                                                                             >  2:759433/1‑76 (MQ=255)
                                                                                                                                            gCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTgcgc     <  4:755129/147‑1 (MQ=255)
                                                                                                                                              tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   >  4:129315/1‑131 (MQ=255)
                                                                                                                                              tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   <  3:129315/131‑1 (MQ=255)
                                                                                                                                              tttCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCACTTCACAGCATTACTTgg                     >  3:1435062/1‑129 (MQ=255)
                                                                                                                                                  aCGCCTTATCCTACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                     <  4:1232546/125‑1 (MQ=255)
                                                                                                                                                  aCGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTgg                     >  3:1232546/1‑125 (MQ=255)
                                                                                                                                                   cGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   >  3:765673/1‑126 (MQ=255)
                                                                                                                                                   cGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTg                   <  4:765673/126‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TGTGCGTACATTAAAACAACAAGGTAACCCACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTC  >  NC_000913/4244966‑4245255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: