Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,347,285 | C→G | 8.3% | M14I (ATG→ATC) | fumB ← | anaerobic class I fumarate hydratase (fumarase B) |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,347,285 | 0 | C | G | 8.3% | 638.0 / 40.2 | 181 | M14I (ATG→ATC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
Reads supporting (aligned to +/- strand): ref base C (78/88); new base G (7/8); total (85/96) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-02 |
Only 100 of 185 total aligned reads displayed.
GAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGC > NC_000913/4347141‑4347427 | aGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCgg < 3:673592/149‑1 (MQ=255) aTAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGaa > 1:669263/1‑149 (MQ=255) tAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGCTGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCGATGGGGaaa < 4:1518633/149‑1 (MQ=255) tAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCGATGGGGaaa > 3:1518633/1‑149 (MQ=255) tAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGaaa > 4:1764119/1‑149 (MQ=255) aaaaGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAg > 3:1641949/1‑149 (MQ=255) aaaaGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAg > 4:1782672/1‑149 (MQ=255) aaaGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAgg < 2:989949/149‑1 (MQ=255) aagCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGcc < 3:244488/149‑1 (MQ=255) tcgtGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTa < 4:1379832/149‑1 (MQ=255) tGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTa > 3:366866/1‑146 (MQ=255) aGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAg > 2:866430/1‑149 (MQ=255) aGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAg < 2:669263/149‑1 (MQ=255) ggCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAgg > 1:1037466/1‑149 (MQ=255) gCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAggg < 1:1815824/149‑1 (MQ=255) cTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgt > 3:1874028/1‑149 (MQ=255) ttGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtt > 1:1849722/1‑149 (MQ=255) tgctgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgttt < 2:641290/149‑1 (MQ=255) gctgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgttt < 3:626143/148‑1 (MQ=255) ctgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCGATGGGGAAAgacaa < 4:795164/129‑5 (MQ=255) ctgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCGATGGGGAAAgacaa > 3:795164/1‑125 (MQ=255) tgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtttgtt < 4:1445463/149‑1 (MQ=255) tgcGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtttgtt > 3:1441152/1‑149 (MQ=255) gcgcCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtttgttt < 3:1764119/149‑1 (MQ=255) cagcaagcagGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtttgttt < 3:1180414/144‑1 (MQ=255) gcCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGa < 4:191656/149‑1 (MQ=255) gcCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGa < 3:1330398/149‑1 (MQ=255) ccAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGAc < 2:1037466/149‑1 (MQ=255) gcagGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGaaa > 2:1172712/1‑116 (MQ=255) gcagGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGaaa < 1:1172712/116‑1 (MQ=255) cagGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAg < 2:1133360/149‑1 (MQ=255) ggTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCtt < 4:1548002/149‑1 (MQ=255) gTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTg > 4:458409/1‑115 (MQ=255) tCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTcc > 2:450849/1‑149 (MQ=255) tCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTcc > 2:580230/1‑149 (MQ=255) cAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCa < 4:1710902/149‑1 (MQ=255) aGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGcc > 4:1212967/1‑114 (MQ=255) aGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAg < 4:1641949/149‑1 (MQ=255) gggCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgtttgttt > 1:204145/1‑134 (MQ=255) ggCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGt < 4:954808/109‑1 (MQ=255) gCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTa > 1:1385177/1‑116 (MQ=255) gCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAg < 1:1565589/123‑1 (MQ=255) gCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCt < 2:1268465/149‑1 (MQ=255) cTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATaaa < 4:249982/121‑1 (MQ=255) tGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGaaa > 3:1544922/1‑100 (MQ=255) ggTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAAct < 2:1077606/149‑1 (MQ=255) ccACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAgg > 1:1081740/1‑114 (MQ=255) aCTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATa < 3:1068449/126‑1 (MQ=255) aCTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATa > 4:1068449/1‑126 (MQ=255) aCTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTAt > 3:1106417/1‑149 (MQ=255) tttCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTaa > 3:1353935/1‑139 (MQ=255) ggATGGTTTCGCCCTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTaa < 4:13503/134‑1 (MQ=255) gATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAg < 3:1804544/86‑1 (MQ=255) gATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTAtt > 2:818645/1‑149 (MQ=255) tttCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAActct < 2:407404/132‑1 (MQ=255) tttCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGaaa < 1:450849/149‑1 (MQ=255) gCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTa < 3:1186638/87‑1 (MQ=255) gCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTa > 4:1186638/1‑87 (MQ=255) gAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGgt > 1:572759/1‑91 (MQ=255) gAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGc < 2:1881539/105‑1 (MQ=255) aaGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCcg > 2:1842916/1‑149 (MQ=255) gTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGGCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTc < 3:979262/132‑1 (MQ=255) cGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTa < 4:1441152/149‑1 (MQ=255) cGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTa < 2:1102826/149‑1 (MQ=255) aCGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCAc < 1:1074677/149‑1 (MQ=255) tAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGggc < 1:1130200/149‑1 (MQ=255) gTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTa < 3:1256397/114‑1 (MQ=255) gTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTa < 1:967432/136‑1 (MQ=255) gTAATCGGAAGTGAGTAGATAGTATCCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTa > 4:1256397/1‑114 (MQ=255) tAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCAc < 4:1106417/149‑1 (MQ=255) aaGTGAGTAGATATTATTAGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTATATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGt < 1:818645/149‑1 (MQ=255) gTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGc < 3:260047/84‑1 (MQ=255) gTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTa > 1:570964/1‑126 (MQ=255) gTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTa < 2:570964/126‑1 (MQ=255) gTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGc > 2:130271/1‑149 (MQ=255) aGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGaaaa > 3:1473128/1‑106 (MQ=255) gATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACAt > 4:520648/1‑67 (MQ=255) gATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGggcg > 3:1702190/1‑129 (MQ=255) aTAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATaa > 1:700698/1‑149 (MQ=255) tATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAActc > 3:241052/1‑81 (MQ=255) tATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAActc < 4:241052/81‑1 (MQ=255) tATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAAc < 2:700698/149‑1 (MQ=255) ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGc > 3:518189/1‑102 (MQ=255) ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCa > 3:309559/1‑116 (MQ=255) ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCa < 4:309559/116‑1 (MQ=255) ttCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCAt < 4:1494359/137‑1 (MQ=255) gTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTa < 2:144760/79‑1 (MQ=255) ttGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGcc < 1:1270743/100‑1 (MQ=255) ttGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGggcg < 1:996994/115‑1 (MQ=255) ttGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGggcg > 2:996994/1‑115 (MQ=255) ttGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATc > 4:1876645/1‑149 (MQ=255) accTTTCCCGATGGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAAt < 4:185000/88‑1 (MQ=255) tCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATcgcg > 4:708698/1‑149 (MQ=255) cTTTCCCGATGGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTaa < 4:361717/65‑1 (MQ=255) cTTTCCCGATGGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTAttt < 1:631086/75‑1 (MQ=255) cTTTCCCGATGGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACg > 2:1629972/1‑107 (MQ=255) tttCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCgcggc > 3:895401/1‑149 (MQ=255) ccATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCAttt > 4:1698135/1‑122 (MQ=255) cATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCAt < 2:1233494/119‑1 (MQ=255) cATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCAt > 1:1233494/1‑119 (MQ=255) | GAGCATAAAAGAAGCGTCGTGAAAGGCTTGCTGCGCCAGCAGGGTCAGGGCTTCTGGTTCCACTTTCAGGATGGTTTCGCCGTCGAAGTCGGCAACGCTAACGTAATCGGAAGTGAGTAGATAGTATTCGGTATTGTCTTTCCCCATCGGGAAAGGTGCCTGGTAGATAAAGGGTTTGTTTGACATAGCTTCCAGCCTGTAACTCTGTATTTGTTATTCGAAAATGGCGTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGC > NC_000913/4347141‑4347427 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |