Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,444,192 C→G 5.0% G27G (GGC→GGG tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,444,1920CG 5.0% 428.1 / 8.6 119G27G (GGC→GGGtamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (60/53);  new base G (3/3);  total (63/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 130 total aligned reads displayed.

TTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATC  >  NC_000913/4444044‑4444340
                                                                                                                                                    |                                                                                                                                                    
tttGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGc                                                                                                                                                      <  2:1582191/149‑1 (MQ=255)
  tGCCGTACCGGTCGCGGAAAAAGACGAACACGGGGTACCGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAATAAAATCAGCCTCGGAGTGGTGATCGTTATCTTACTGTTGCCGGGATCGGTGGCGCTTCTGGTGGGcac                                                                                                                                                    <  2:1863544/149‑1 (MQ=255)
             tCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGcc                                                                                                                                         <  1:768194/149‑1 (MQ=255)
                   aTAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGcacca                                                                                                                                                  <  3:1783151/134‑1 (MQ=255)
                   aTAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGAGcacca                                                                                                                                                  >  4:1783151/1‑134 (MQ=255)
                       aGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGt                                                                                                                               >  4:588693/1‑149 (MQ=255)
                        gACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGTGCCCACCAgaaa                                                                                                                                           >  3:1761862/1‑133 (MQ=255)
                        gACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTa                                                                                                                              <  2:1300488/149‑1 (MQ=255)
                           gAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGTGCCCACCAgaa                                                                                                                                            >  3:740239/1‑130 (MQ=255)
                              cacGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGTGCCCACCAgaaac                                                                                                                                          >  4:380777/1‑127 (MQ=255)
                              cacGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGTGCCCACCAgaaac                                                                                                                                          <  3:380777/131‑5 (MQ=255)
                                 gggTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcg                                                                                                                     >  2:235585/1‑149 (MQ=255)
                                   gTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcggc                                                                                                                   <  3:275878/149‑1 (MQ=255)
                                        aGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATc                                                                                                              >  4:1317527/1‑149 (MQ=255)
                                        aGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATc                                                                                                              >  1:1610732/1‑149 (MQ=255)
                                        aGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATc                                                                                                              >  3:1638422/1‑149 (MQ=255)
                                         gTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAg                                                                                                                       >  1:359206/1‑139 (MQ=255)
                                           tttACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCt                                                                                                           <  2:1218678/149‑1 (MQ=255)
                                             tACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         <  1:1854465/149‑1 (MQ=255)
                                             tACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         <  1:235585/149‑1 (MQ=255)
                                             tACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCACGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCCgg                                                                                                         <  4:1265820/149‑1 (MQ=255)
                                               cATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGt                                                                                                       <  3:1317527/149‑1 (MQ=255)
                                                aTCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTg                                                                                                      >  1:1236361/1‑149 (MQ=255)
                                                           gCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGa                                                                                           >  3:1789432/1‑149 (MQ=255)
                                                            ccAGAATTATGAGTTTATGCAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGat                                                                                          >  3:1117608/1‑149 (MQ=255)
                                                               gAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAg                                                                                                  >  4:1435379/1‑138 (MQ=255)
                                                                aaTTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTg                                                                                      <  2:1610732/149‑1 (MQ=255)
                                                                aaTTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTg                                                                                      <  4:1554384/149‑1 (MQ=255)
                                                                  ttATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGc                                                                                    <  4:1638422/149‑1 (MQ=255)
                                                                        gTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTggg                                                                                                        >  4:1547647/1‑123 (MQ=255)
                                                                        gTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTggg                                                                                                        <  3:1547647/123‑1 (MQ=255)
                                                                         tttATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACTAGCGGCCTGCATCTgg                                                                                                                                >  1:1206061/1‑98 (MQ=255)
                                                                         tttATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTAttt                                                                                                                           >  2:184701/1‑103 (MQ=255)
                                                                             tGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         >  2:731709/1‑117 (MQ=255)
                                                                             tGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         <  1:731709/117‑1 (MQ=255)
                                                                              ggAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTc                                                                              <  1:1734071/143‑1 (MQ=255)
                                                                                aaaaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTc                                                                              >  1:1826756/1‑141 (MQ=255)
                                                                                aaaaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTc                                                                              <  2:1826756/141‑1 (MQ=255)
                                                                                aaaaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACcggcgg                                                                      >  3:1495284/1‑149 (MQ=255)
                                                                                aaaaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACcggcgg                                                                      >  2:409612/1‑149 (MQ=255)
                                                                                 aaaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCggcggc                                                                     >  1:657001/1‑149 (MQ=255)
                                                                                  aaaaaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgc                                                                                                                      <  4:324416/99‑1 (MQ=255)
                                                                                     aaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTg                                                                                                                                 <  4:163404/85‑1 (MQ=255)
                                                                                     aaTCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTg                                                                                                                                 >  3:163404/1‑85 (MQ=255)
                                                                                                gCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCGCCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGt                                                                                                       >  2:486607/1‑100 (MQ=255)
                                                                                                gCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGt                                                                                                       <  1:486607/100‑1 (MQ=255)
                                                                                                 cGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCt                                                     <  1:409612/149‑1 (MQ=255)
                                                                                                  gTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCtt                                                    <  4:813177/149‑1 (MQ=255)
                                                                                                    ggTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGt                                                  <  2:1236361/149‑1 (MQ=255)
                                                                                                     gttatcgttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         >  1:1551833/1‑93 (MQ=255)
                                                                                                     gttatcgttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTgg                                                                                                         <  2:1551833/93‑1 (MQ=255)
                                                                                                      ttatcgttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCt                                                >  3:1116540/1‑149 (MQ=255)
                                                                                                          cgttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcg                                            >  4:1540194/1‑149 (MQ=255)
                                                                                                            ttatcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgtt                                          >  2:1634066/1‑149 (MQ=255)
                                                                                                               tcTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcgt                                       <  4:158292/149‑1 (MQ=255)
                                                                                                                cTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcgt                                       <  3:1672538/148‑1 (MQ=255)
                                                                                                                cTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcgt                                       >  4:1672538/1‑148 (MQ=255)
                                                                                                                 ttACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAg                                                                                 <  1:790505/105‑1 (MQ=255)
                                                                                                                 ttACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAg                                                                                 >  2:790505/1‑105 (MQ=255)
                                                                                                                 ttACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGt                                                                               >  1:928604/1‑107 (MQ=255)
                                                                                                                 ttACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATAGTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTa                                     >  2:702320/1‑149 (MQ=255)
                                                                                                                     tGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGag                                 >  1:88838/1‑149 (MQ=255)
                                                                                                                       ttGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttc                                         >  4:846500/1‑139 (MQ=255)
                                                                                                                       ttGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttc                                         <  3:846500/139‑1 (MQ=255)
                                                                                                                       ttGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGagca                               <  2:657001/149‑1 (MQ=255)
                                                                                                                            gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTg                                                                                      <  4:678238/89‑1 (MQ=255)
                                                                                                                            gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTg                                                                                      >  3:678238/1‑89 (MQ=255)
                                                                                                                            gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGgc                                                                 <  2:1413939/110‑1 (MQ=255)
                                                                                                                            gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGgc                                                                 >  1:1413939/1‑110 (MQ=255)
                                                                                                                            gggATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAg                          >  4:1819096/1‑149 (MQ=255)
                                                                                                                               aTCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttc                                         >  3:521188/1‑131 (MQ=255)
                                                                                                                               aTCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGc                        >  4:489380/1‑148 (MQ=255)
                                                                                                                               aTCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGc                        <  3:489380/148‑1 (MQ=255)
                                                                                                                               aTCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCg                       >  1:1777088/1‑149 (MQ=255)
                                                                                                                               aTCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCg                       >  1:723349/1‑149 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTg                                                                                             >  3:663151/1‑74 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTg                                                                                             <  4:663151/74‑1 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTAt                                    >  1:1401651/1‑131 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTAt                                    <  2:1401651/131‑1 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTg                     <  1:1365270/146‑1 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCg                  >  1:1393815/1‑149 (MQ=255)
                                                                                                                                    tGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCg                  >  3:1507082/1‑149 (MQ=255)
                                                                                                                                      gCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCaa                                                                                  <  1:1057269/83‑1 (MQ=255)
                                                                                                                                      gCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCaa                                                                                  >  2:1057269/1‑83 (MQ=255)
                                                                                                                                        gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcgg                                                                                                                    <  2:1472414/47‑1 (MQ=255)
                                                                                                                                        gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcgg                                                                                                                    >  1:1472414/1‑47 (MQ=255)
                                                                                                                                        gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaa              >  3:1144468/1‑149 (MQ=255)
                                                                                                                                        gTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaa              >  3:1853178/1‑149 (MQ=255)
                                                                                                                                         tttCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTaaa             >  2:1543792/1‑149 (MQ=255)
                                                                                                                                             tggtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCg         <  4:1507082/149‑1 (MQ=255)
                                                                                                                                              ggtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCgg        <  2:1374829/149‑1 (MQ=255)
                                                                                                                                              ggtggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCgg        <  4:1116540/149‑1 (MQ=255)
                                                                                                                                                tggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcg                                        <  3:724722/115‑1 (MQ=255)
                                                                                                                                                tggGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGAcgttcg                                        >  4:724722/1‑115 (MQ=255)
                                                                                                                                                  ggCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCaa    <  2:1393815/149‑1 (MQ=255)
                                                                                                                                                    caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCgg        >  3:999733/1‑143 (MQ=255)
                                                                                                                                                    caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATc  <  4:1769570/149‑1 (MQ=255)
                                                                                                                                                    caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATc  >  3:251919/1‑149 (MQ=255)
                                                                                                                                                    caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATc  >  1:1564577/1‑149 (MQ=255)
                                                                                                                                                    caccacCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATc  >  3:1769570/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCGCTGGGTGCCAGGACTGGATATTGGCAAGGTCACCGGCGGCTGGCGCGATCTCACCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATC  >  NC_000913/4444044‑4444340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: