Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,496,511 A→G 6.8% intergenic (+22/‑164) leuX → / → intB tRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,496,5110AG 6.8% 479.6 / 18.9 146intergenic (+22/‑164)leuX/intBtRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
Reads supporting (aligned to +/- strand):  ref base A (69/67);  new base G (5/5);  total (74/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 151 total aligned reads displayed.

CGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAA  >  NC_000913/4496363‑4496659
                                                                                                                                                    |                                                                                                                                                    
cGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCaa                                                                                                                                                      <  1:1545471/149‑1 (MQ=255)
 gACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAAc                                                                                                                                                     >  4:734310/1‑149 (MQ=255)
   cAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTg                                                                                                                                                   <  1:477608/149‑1 (MQ=255)
   cAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTg                                                                                                                                                   <  3:566361/149‑1 (MQ=255)
     ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 <  1:1282422/149‑1 (MQ=255)
      gTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAgg                                                                                                                                                >  3:764395/1‑149 (MQ=255)
         taATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCaa                                                                                                                                                      <  2:1200751/140‑1 (MQ=255)
         taATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCaa                                                                                                                                                      >  1:1200751/1‑140 (MQ=255)
           aTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat                                                                                                                                           <  1:1020645/149‑3 (MQ=255)
            tCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttt                                                                                                                                          <  2:1838229/149‑1 (MQ=255)
            tCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttt                                                                                                                                          <  4:685157/149‑1 (MQ=255)
            tCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttt                                                                                                                                          <  2:268145/149‑1 (MQ=255)
               acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 <  2:595476/139‑1 (MQ=255)
               acaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg                                                                                                                                                 >  1:595476/1‑139 (MQ=255)
                caACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTa                                                                                                                                      >  3:184886/1‑149 (MQ=255)
                 aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTc                                                                                                                                              <  2:138329/140‑1 (MQ=255)
                 aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTc                                                                                                                                              >  1:138329/1‑140 (MQ=255)
                 aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTAt                                                                                                                                     >  1:205378/1‑149 (MQ=255)
                 aaCGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTAt                                                                                                                                     >  2:1142906/1‑149 (MQ=255)
                          cGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtt                                                                                                                                            >  2:1384767/1‑133 (MQ=255)
                              tACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAg                                                                                                                        >  4:1770782/1‑149 (MQ=255)
                               aCCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgt                                                                                                                       >  4:511663/1‑149 (MQ=255)
                               aCCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgt                                                                                                                       <  1:1142906/149‑1 (MQ=255)
                               aCCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgt                                                                                                                       <  3:511663/149‑1 (MQ=255)
                                 ctctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     >  1:216747/1‑149 (MQ=255)
                                 ctctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt                                                                                                                     >  3:908714/1‑149 (MQ=255)
                                  tctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                    >  2:220538/1‑149 (MQ=255)
                                  tctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                    >  2:1580438/1‑149 (MQ=255)
                                      cAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTTAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatc                                                                                                                >  2:361138/1‑149 (MQ=255)
                                       aGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatct                                                                                                               <  4:908714/149‑1 (MQ=255)
                                         tGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctat                                                                                                             <  1:801706/149‑1 (MQ=255)
                                                  ggCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt                                                                                                                   <  2:848138/134‑1 (MQ=255)
                                                   gCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       <  3:265560/113‑1 (MQ=255)
                                                   gCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  4:265560/1‑113 (MQ=255)
                                                   gCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCt                                                                                                   >  1:224274/1‑149 (MQ=255)
                                                   gCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCt                                                                                                   <  1:1462828/149‑1 (MQ=255)
                                                     gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatc                                                                                                                <  2:324711/134‑1 (MQ=255)
                                                     gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatc                                                                                                                >  1:324711/1‑134 (MQ=255)
                                                     gAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtat                                                                                                 >  3:1368142/1‑149 (MQ=255)
                                                      aaaTCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCtata                                                                                                >  1:1056626/1‑149 (MQ=255)
                                                       aaTCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTatat                                                                                               <  2:1525058/149‑1 (MQ=255)
                                                        aTCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATAtt                                                                                              <  1:1580438/149‑1 (MQ=255)
                                                          cGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa                                                                                            <  3:1770782/149‑1 (MQ=255)
                                                                aCGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGaaa                                                                                                                             >  2:1161187/1‑110 (MQ=255)
                                                                aCGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGaaa                                                                                                                             <  1:1161187/110‑1 (MQ=255)
                                                                  gCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCa                                                                                    >  4:725839/1‑149 (MQ=255)
                                                                     gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAgg                                                                                 <  4:1368142/149‑1 (MQ=255)
                                                                      ttGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGt                                                                                >  4:1804730/1‑149 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt                                                                                                                   <  4:1684483/110‑1 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt                                                                                                                   >  3:1684483/1‑110 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                           <  4:1573599/134‑1 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                           >  3:1573599/1‑134 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAAc                                                                            <  2:205378/149‑1 (MQ=255)
                                                                            cAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAAcc                                                                           <  4:383459/148‑1 (MQ=255)
                                                                            cAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAAcc                                                                           >  3:383459/1‑148 (MQ=255)
                                                                             aaaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       <  2:566191/87‑1 (MQ=255)
                                                                             aaaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  1:566191/1‑87 (MQ=255)
                                                                              aaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGat                                                                        <  3:725839/149‑1 (MQ=255)
                                                                                 tCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  1:715117/1‑83 (MQ=255)
                                                                                 tCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       <  2:414867/83‑1 (MQ=255)
                                                                                 tCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       <  2:715117/83‑1 (MQ=255)
                                                                                 tCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttttt                                                                                                                                       >  1:414867/1‑83 (MQ=255)
                                                                                             aTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCaa                                                                   <  4:582832/139‑1 (MQ=255)
                                                                                              tACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTAc                                                        >  3:747099/1‑149 (MQ=255)
                                                                                                   gCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActctc                                                                                       <  4:53219/113‑1 (MQ=255)
                                                                                                   gCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActctc                                                                                       >  3:53219/1‑113 (MQ=255)
                                                                                                             gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTa                                         <  4:365235/149‑1 (MQ=255)
                                                                                                              tCCGGCCTTCGTCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTAc                                        >  3:1083479/1‑149 (MQ=255)
                                                                                                                   ccTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTa                                         >  4:1010583/1‑143 (MQ=255)
                                                                                                                    cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  2:224274/149‑1 (MQ=255)
                                                                                                                    cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  4:681964/149‑1 (MQ=255)
                                                                                                                    cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  4:1083479/149‑1 (MQ=255)
                                                                                                                     ttCGGTACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatgat                                 >  3:36522/1‑149 (MQ=255)
                                                                                                                      tCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGatgatg                                <  2:1309948/149‑1 (MQ=255)
                                                                                                                         gCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGtt                                             >  3:182580/1‑133 (MQ=255)
                                                                                                                         gCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGtt                                             <  4:182580/133‑1 (MQ=255)
                                                                                                                           aCCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctatc                                                                                                            >  4:1890784/1‑68 (MQ=255)
                                                                                                                             cAAAAGTATGTGAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                           >  3:1637795/1‑83 (MQ=255)
                                                                                                                             cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaag                                                                                           <  4:1637795/83‑2 (MQ=255)
                                                                                                                               aaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCg                       >  1:1426989/1‑149 (MQ=255)
                                                                                                                                   tATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTc                   <  2:1056626/149‑1 (MQ=255)
                                                                                                                                     tGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGatgatg                                >  1:1214441/1‑134 (MQ=255)
                                                                                                                                     tGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGatgatg                                <  2:1214441/134‑1 (MQ=255)
                                                                                                                                       tAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTg                                            >  3:1788054/1‑120 (MQ=255)
                                                                                                                                       tAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTg                                            <  4:1788054/120‑1 (MQ=255)
                                                                                                                                         aaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAAt             >  1:96977/1‑149 (MQ=255)
                                                                                                                                         aaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAAt             >  3:937584/1‑149 (MQ=255)
                                                                                                                                            aGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgat                                    <  3:424389/123‑1 (MQ=255)
                                                                                                                                            aGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgat                                    <  3:1198093/123‑1 (MQ=255)
                                                                                                                                            aGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgat                                    >  4:1198093/1‑123 (MQ=255)
                                                                                                                                              aCCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTg                                            >  1:32737/1‑113 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTa                                                               <  3:1593889/92‑1 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTa                                                               >  4:1593889/1‑92 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACata                                                 <  3:663044/106‑1 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  >  2:1289274/1‑121 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                  <  1:1289274/121‑1 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATgg                               <  1:238732/124‑1 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGtt                    <  1:1341875/135‑1 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGa                 >  3:570838/1‑138 (MQ=255)
                                                                                                                                                cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGCACAGATGATGGTATTTCCGGTTCGa                 <  4:570838/138‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAA  >  NC_000913/4496363‑4496659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: