Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,932 T→A 11.0% intergenic (+222/+21) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,9320TA11.0% 260.0 / 18.6 81intergenic (+222/+21)fimH/gntPminor component of type 1 fimbriae/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base A (5/4);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAA  >  NC_000913/4549788‑4550079
                                                                                                                                                |                                                                                                                                                   
ccAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                                                                                 <  3:335003/149‑1 (MQ=255)
  aGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAg                                                                                                                                               >  1:1436998/1‑149 (MQ=255)
         aCGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                                                                                        >  4:934047/1‑149 (MQ=255)
              tCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTcc                                                                                                                                   <  3:934047/149‑1 (MQ=255)
                   gTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAt                                                                                                                                                     >  1:1225641/1‑126 (MQ=255)
                   gTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAt                                                                                                                                                     <  2:1225641/126‑1 (MQ=255)
                               aTAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                                                                                  >  3:1866860/1‑149 (MQ=255)
                                tAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                                                                                                                   <  2:452523/115‑1 (MQ=255)
                                tAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                                                                                                                   >  1:452523/1‑115 (MQ=255)
                                            aCTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                                                                                 <  3:62099/105‑1 (MQ=255)
                                            aCTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                                                                                 >  4:62099/1‑105 (MQ=255)
                                            aCTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                                                                                    >  4:1640892/1‑118 (MQ=255)
                                            aCTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                                                                                    <  3:1640892/118‑1 (MQ=255)
                                                    ggTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAg                                                                                             >  3:198337/1‑149 (MQ=255)
                                                     gTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            <  4:1866860/149‑1 (MQ=255)
                                                      tCTATTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                             >  4:1375321/1‑115 (MQ=255)
                                                      tCTATTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                             <  3:1375321/115‑1 (MQ=255)
                                                         gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            >  1:1793870/1‑145 (MQ=255)
                                                         gTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            <  2:1793870/145‑1 (MQ=255)
                                                          ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaa                                                                                                    <  4:1397032/136‑1 (MQ=255)
                                                          ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaa                                                                                                    >  3:1397032/1‑136 (MQ=255)
                                                          ttCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGACCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAAc                                                                                       >  3:1319711/1‑149 (MQ=255)
                                                                tCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGa                                                                                 <  4:198337/149‑1 (MQ=255)
                                                                  aCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctca                                                                                                                                          >  3:633155/1‑89 (MQ=255)
                                                                  aCCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctca                                                                                                                                          <  4:633155/90‑2 (MQ=255)
                                                                       aaCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAcc                                                                          >  1:405273/1‑149 (MQ=255)
                                                                          ccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                             <  2:1809843/95‑1 (MQ=255)
                                                                          ccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                                                                             >  1:1809843/1‑95 (MQ=255)
                                                                           ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                                                                           <  4:932985/128‑1 (MQ=255)
                                                                           ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGcc                                                                                           >  3:932985/1‑128 (MQ=255)
                                                                           ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                                                                   <  2:421466/136‑1 (MQ=255)
                                                                           ctctCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACg                                                                                   >  1:421466/1‑136 (MQ=255)
                                                                            tctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                           >  2:530932/1‑143 (MQ=255)
                                                                            tctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                                                                           <  1:530932/143‑1 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                                                                   >  2:538539/1‑101 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                                                                   <  1:538539/101‑1 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaat                                                                                                   >  4:336631/1‑117 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaat                                                                                                   <  3:336631/117‑1 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCca                                                                   <  2:405273/149‑1 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGc                                                                                                                                             >  1:1590947/1‑75 (MQ=255)
                                                                              tcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGc                                                                                                                                             <  2:1590947/75‑1 (MQ=255)
                                                                                cccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                                                                   >  4:82627/1‑99 (MQ=255)
                                                                                cccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                                                                                   <  3:82627/99‑1 (MQ=255)
                                                                                cccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAgg                                                                                                                                              <  2:322226/72‑1 (MQ=255)
                                                                                cccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAgg                                                                                                                                              >  1:322226/1‑72 (MQ=255)
                                                                                 ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            >  4:581587/1‑121 (MQ=255)
                                                                                 ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            <  3:581587/121‑1 (MQ=255)
                                                                                              gggACTGACCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGtttt                                                   <  4:1319711/149‑1 (MQ=255)
                                                                                                 aCTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                         <  1:790216/60‑1 (MQ=255)
                                                                                                 aCTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTg                                                                                                                                         >  2:790216/1‑60 (MQ=255)
                                                                                                            acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaa                                                                                                    <  4:1318995/86‑1 (MQ=255)
                                                                                                            acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACaa                                                                                                    >  3:1318995/1‑86 (MQ=255)
                                                                                                            acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            >  4:1250079/1‑94 (MQ=255)
                                                                                                            acaTTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGc                                                                                            <  3:1250079/94‑1 (MQ=255)
                                                                                                               tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                            <  4:1276691/107‑1 (MQ=255)
                                                                                                               tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                            <  1:1195789/107‑1 (MQ=255)
                                                                                                               tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                            >  2:1195789/1‑107 (MQ=255)
                                                                                                               tttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGa                                                                            >  3:1276691/1‑107 (MQ=255)
                                                                                                                ttttAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGa                                 <  2:1436998/149‑1 (MQ=255)
                                                                                                                      aCTTTGTCGTCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGa                                                                                 >  3:1741915/1‑95 (MQ=255)
                                                                                                                      aCTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGa                                                                                 <  4:1741915/95‑1 (MQ=255)
                                                                                                                             catcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTaa                    >  3:105445/1‑149 (MQ=255)
                                                                                                                              atcaGTCTTAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCATCCCAACCGCGGAGTTGACCAGCTCCAGCAGACCCCAGGtttt                                                   <  2:838417/117‑1 (MQ=255)
                                                                                                                              atcaGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGtttt                                                   >  1:838417/1‑117 (MQ=255)
                                                                                                                                     agAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTAc                                    <  2:662993/124‑1 (MQ=255)
                                                                                                                                     agAGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTAc                                    >  1:662993/2‑125 (MQ=255)
                                                                                                                                      gAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaac           >  2:252/1‑149 (MQ=255)
                                                                                                                                       aGCCTGCCAATGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaaca          >  4:186577/1‑149 (MQ=255)
                                                                                                                                         ccTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCaa                                                <  2:1501288/109‑1 (MQ=255)
                                                                                                                                         ccTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCaa                                                >  1:1501288/1‑109 (MQ=255)
                                                                                                                                          cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaacaa         <  1:1160993/147‑1 (MQ=255)
                                                                                                                                          cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGaacaa         >  2:1160993/1‑147 (MQ=255)
                                                                                                                                          cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAAcc       >  3:1486017/1‑149 (MQ=255)
                                                                                                                                          cTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAAcc       >  2:1832462/1‑149 (MQ=255)
                                                                                                                                           tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCa      >  3:1539237/1‑149 (MQ=255)
                                                                                                                                           tGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCa      >  1:1394501/1‑149 (MQ=255)
                                                                                                                                              cATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGt                            >  3:232698/1‑124 (MQ=255)
                                                                                                                                              cATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGt                            <  4:232698/124‑1 (MQ=255)
                                                                                                                                              cATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaa   >  4:207248/1‑149 (MQ=255)
                                                                                                                                               aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCaaa                        >  4:1798615/1‑127 (MQ=255)
                                                                                                                                               aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCaaa                        <  3:1798615/127‑1 (MQ=255)
                                                                                                                                               aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaaa  <  4:1539237/149‑1 (MQ=255)
                                                                                                                                               aTTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGaaa  >  2:612119/1‑149 (MQ=255)
                                                                                                                                                ttGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCttt               >  4:770799/1‑135 (MQ=255)
                                                                                                                                                ttGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCttt               <  3:770799/135‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGACCCCAGGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAA  >  NC_000913/4549788‑4550079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: