Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,572,070 G→A 5.4% intergenic (+155/‑344) yjiS → / → yjiT DUF1127 family protein/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,572,0700GA 5.4% 436.5 / 8.7 127intergenic (+155/‑344)yjiS/yjiTDUF1127 family protein/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (62/58);  new base A (4/3);  total (66/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 129 total aligned reads displayed.

ATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAA  >  NC_000913/4571936‑4572216
                                                                                                                                      |                                                                                                                                                   
ataataAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      >  4:1168106/1‑149 (MQ=255)
  aataaAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTtgt                                                                                                                                    >  1:1628175/1‑149 (MQ=255)
       aaCGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTgtgt                                                                                                                                  >  4:968932/1‑146 (MQ=255)
       aaCGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTgtgt                                                                                                                                  <  3:968932/146‑1 (MQ=255)
                   tttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACg                                                                                                                                                     >  2:248065/1‑116 (MQ=255)
                   tttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACg                                                                                                                                                     <  1:248065/116‑1 (MQ=255)
                    ttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACACAAGTAATCACTGTGCGTTTTTtga                                                                                                                           >  1:650847/1‑140 (MQ=38)
                    ttttAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACACAAGTAATCACTGTGCGTTTTTtga                                                                                                                           <  2:650847/140‑1 (MQ=38)
                             aaGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTATAACTCTCa                                                                                                         <  3:1168106/149‑1 (MQ=255)
                                ttATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCatta                                                                                                      >  4:486634/1‑149 (MQ=255)
                                  ataAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGAt                                                                                                                                            >  4:1325573/1‑110 (MQ=255)
                                  ataAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGAt                                                                                                                                            <  3:1325573/110‑1 (MQ=255)
                                   taAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTc                                                                                                   <  2:1628175/149‑1 (MQ=255)
                                     aaaaTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTGTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTtga                                                                                                                           >  4:1097914/1‑123 (MQ=255)
                                     aaaaTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTtga                                                                                                                           <  3:1097914/123‑1 (MQ=255)
                                       aaTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      >  2:747727/1‑110 (MQ=255)
                                       aaTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      <  1:747727/110‑1 (MQ=255)
                                        aTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGtttttt                                                                                                                             <  2:1739753/118‑1 (MQ=255)
                                        aTTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGtttttt                                                                                                                             >  1:1739753/1‑118 (MQ=255)
                                            tCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                    <  1:208015/139‑1 (MQ=255)
                                            tCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCattatt                                                                                                    >  2:208015/1‑139 (MQ=255)
                                               cGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAActct                                                                                                           <  3:1152132/129‑1 (MQ=255)
                                               cGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAg                                                                                       >  4:1163740/1‑149 (MQ=255)
                                                gagaCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTa                                                                                                                  <  2:1858024/121‑1 (MQ=255)
                                                gagaCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTa                                                                                                                  >  1:1858024/1‑121 (MQ=255)
                                                gagaCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGt                                                                                      >  1:787060/1‑149 (MQ=255)
                                                gagaCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGt                                                                                      >  2:1564810/1‑149 (MQ=255)
                                                   aCAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAAct                                                                                                             <  1:682157/123‑1 (MQ=255)
                                                     aaTTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCat                                                                                                        <  4:1697032/126‑1 (MQ=255)
                                                     aaTTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCat                                                                                                        >  3:1697032/1‑126 (MQ=255)
                                                     aaTTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCat                                                                                                        <  2:227018/126‑1 (MQ=255)
                                                     aaTTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCat                                                                                                        >  1:227018/1‑126 (MQ=255)
                                                        tcatcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTaa                                                                              <  3:486634/149‑1 (MQ=255)
                                                          atcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTtgac                                                                                                                          >  2:1471384/1‑103 (MQ=255)
                                                          atcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTtgac                                                                                                                          <  1:1471384/103‑1 (MQ=255)
                                                           tcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGttttt                                                                                                                              <  2:276638/98‑1 (MQ=255)
                                                           tcaATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGttttt                                                                                                                              >  1:276638/1‑98 (MQ=255)
                                                             aaTAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGt                                                                                                                                               <  1:689958/80‑1 (MQ=255)
                                                             aaTAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGt                                                                                                                                               >  2:689958/1‑80 (MQ=255)
                                                              aTAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGc                                                                                         <  2:1686514/132‑1 (MQ=255)
                                                              aTAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGc                                                                                         >  1:1686514/1‑132 (MQ=255)
                                                              aTAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGttt                                                                        <  2:787060/149‑1 (MQ=255)
                                                                       gAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      <  1:88847/78‑1 (MQ=255)
                                                                        aGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTgg                                                              >  1:1503112/1‑149 (MQ=255)
                                                                             aCAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGttt                                                                                    <  2:257851/122‑1 (MQ=255)
                                                                              cAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGtt                                                        >  3:1465421/1‑149 (MQ=255)
                                                                                ggAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaa                                                      <  1:1564810/149‑1 (MQ=255)
                                                                                 gAATATCTTCTTCACTAACCTCTTTAAACTTATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGGTATTGTGCAGTTtc                                                                                   >  4:12623/1‑119 (MQ=255)
                                                                                 gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTtc                                                                                   <  3:12623/119‑1 (MQ=255)
                                                                                 gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     >  1:1806470/1‑149 (MQ=255)
                                                                                 gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     >  3:992131/1‑149 (MQ=255)
                                                                                 gAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     >  3:558636/1‑149 (MQ=255)
                                                                                      tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGtttttt                                                                                                                             >  1:618360/1‑72 (MQ=255)
                                                                                      tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGtttttt                                                                                                                             <  2:618360/72‑1 (MQ=255)
                                                                                      tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaaa                                                           <  4:52118/138‑1 (MQ=255)
                                                                                      tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaaa                                                           <  4:298799/138‑1 (MQ=255)
                                                                                      tcttctTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGaaa                                                           >  3:298799/1‑138 (MQ=255)
                                                                                        ttcttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     >  1:477097/1‑142 (MQ=255)
                                                                                        ttcttcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     <  2:477097/142‑1 (MQ=255)
                                                                                            tcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAAt                                                    >  3:119398/1‑139 (MQ=255)
                                                                                            tcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAAt                                                    <  4:119398/139‑1 (MQ=255)
                                                                                            tcACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACt                                          <  4:1465421/149‑1 (MQ=255)
                                                                                              aCTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      >  3:1032777/1‑55 (MQ=255)
                                                                                              aCTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACtt                                                                                                                                      <  4:1032777/55‑1 (MQ=255)
                                                                                                tAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     <  2:810959/134‑1 (MQ=255)
                                                                                                tAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTaaa                                                     >  1:810959/1‑134 (MQ=255)
                                                                                                 aaCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACACAAGTAATCACTGTGCGTTTTTtg                                                                                                                            >  2:901346/1‑62 (MQ=2)
                                                                                                 aaCCTCTTTAAACTCATGTCGATAACGGTCAAAAAACACAAGTAATCACTGTGCGTTTTTtg                                                                                                                            <  1:901346/62‑1 (MQ=2)
                                                                                                   ccTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGttt                                                                        <  4:1773675/112‑1 (MQ=255)
                                                                                                   ccTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGttt                                                                        >  3:1773675/1‑112 (MQ=255)
                                                                                                     tctTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACttt                                 >  1:1319253/1‑149 (MQ=255)
                                                                                                       tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACtttt                                <  3:1332846/148‑1 (MQ=255)
                                                                                                       tttAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACtttt                                >  4:1332846/1‑148 (MQ=255)
                                                                                                          aaaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                       >  4:977413/1‑106 (MQ=255)
                                                                                                          aaaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                       <  3:879153/106‑1 (MQ=255)
                                                                                                          aaaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGtttt                                                                       >  4:879153/1‑106 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTGAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGAtt                           <  1:1155505/149‑1 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTAc                                           >  1:1304099/1‑133 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTAc                                           <  2:1304099/133‑1 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGAtt                           >  2:1167002/1‑149 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGAtt                           <  1:1167002/149‑1 (MQ=255)
                                                                                                           aaCTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGAtt                           <  2:1503112/149‑1 (MQ=255)
                                                                                                              tCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGt                                                         <  2:69694/116‑1 (MQ=255)
                                                                                                               cATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGttt                       >  2:565764/1‑149 (MQ=255)
                                                                                                                   tCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTgaga                   <  2:1319253/149‑1 (MQ=255)
                                                                                                                     gATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGatt                 >  1:236763/1‑149 (MQ=255)
                                                                                                                       tAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGttt                       >  3:253134/1‑141 (MQ=255)
                                                                                                                       tAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGttt                       <  4:253134/141‑1 (MQ=255)
                                                                                                                        aaCGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGAtt                           <  1:790945/136‑1 (MQ=255)
                                                                                                                            gTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTc          >  3:314284/1‑149 (MQ=255)
                                                                                                                            gTCAAAAAACACAAGTAATCACTGTGCGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTga                     <  4:264189/138‑1 (MQ=38)
                                                                                                                            gTCAAAAAACACAAGTAATCACTGTGCGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTga                     >  3:264189/1‑138 (MQ=38)
                                                                                                                             tCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACt                                          >  1:1616385/1‑116 (MQ=255)
                                                                                                                             tCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACt                                          <  2:1616385/116‑1 (MQ=255)
                                                                                                                             tCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCa         >  1:1829098/1‑149 (MQ=255)
                                                                                                                               aaaaaaCGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTg                                         >  1:804061/1‑115 (MQ=255)
                                                                                                                               aaaaaaCGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTg                                         <  2:804061/115‑1 (MQ=255)
                                                                                                                               aaaaaaCGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCAtt       >  1:410017/1‑149 (MQ=255)
                                                                                                                                   aaCGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGaa   >  3:544770/1‑149 (MQ=255)
                                                                                                                                    aCGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGaaa  <  2:796152/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                   
ATAATAAAACGTTTTTATGTTTTTAAATTAAGTTATAAAAATTTTCCCGAGACAATTCATCAATAGGTATGGAGTGTACAGGAATATCTTCTTCACTAACCTCTTTAAACTCATGTCGATAACGGTCAAAAAACGCACAGTGATTACTTGTGTTTTTTGACTGACTTTACAACTCTCATTATTCGCTATTGTGCAGTTTCTCTAATTGTTTTATACCCTGGAAAGTTAAATGTCAGCTACTGAATACTTTTTGATTGTTTGAGATTTATTTTCATTTGAAA  >  NC_000913/4571936‑4572216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: