| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,612,330 | G→C | 15.1% | intergenic (+41/‑81) | ytjA → / → yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,612,330 | 0 | G | C | 15.1% | 153.5 / 21.0 | 53 | intergenic (+41/‑81) | ytjA/yjjU | uncharacterized protein/putative patatin‑like family phospholipase |
| Reads supporting (aligned to +/- strand): ref base G (23/22); new base C (4/4); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTG > NC_000913/4612186‑4612477 | ttGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGa < 1:795060/149‑1 (MQ=255) ttGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGCTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTAc > 2:134486/1‑149 (MQ=255) tttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCTTTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGc < 1:1214702/125‑1 (MQ=255) tttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGc > 2:1214702/1‑125 (MQ=255) gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAGTAAATTGCGTTACTTTGTCATTCTTTcac < 1:134486/149‑1 (MQ=255) ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGtttt > 4:1695623/1‑101 (MQ=255) ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGtttt < 3:1695623/101‑1 (MQ=255) gTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg > 1:1818504/1‑113 (MQ=255) gTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg < 2:1818504/113‑1 (MQ=255) tttGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCagag > 3:1318817/1‑149 (MQ=255) gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCagag > 1:1530122/1‑146 (MQ=255) gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCagag < 2:1530122/146‑1 (MQ=255) aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAataata < 4:442104/94‑1 (MQ=255) aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAataata > 3:442104/1‑94 (MQ=255) ggCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc > 2:1793491/1‑149 (MQ=255) aaaaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATa < 1:1793491/149‑1 (MQ=255) aaaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCttt < 1:1447462/65‑1 (MQ=37) aaaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCT‑GGACTGGCttt > 2:1447462/1‑65 (MQ=37) aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg < 4:1227991/136‑1 (MQ=255) aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg > 3:1227991/1‑136 (MQ=255) aCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAAGAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt < 4:1318817/149‑1 (MQ=255) gACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca < 1:1327548/107‑1 (MQ=255) gACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGATTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca > 2:1327548/1‑107 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc > 2:228923/1‑135 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc < 1:228923/135‑1 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATAc > 4:1805513/1‑141 (MQ=255) cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATAc < 3:1805513/141‑1 (MQ=255) aGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg < 2:1654550/127‑1 (MQ=255) aGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg > 1:1654550/1‑127 (MQ=255) gACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTggg < 2:1108120/120‑1 (MQ=255) gACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTggg > 1:1108120/1‑120 (MQ=255) cATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATAc > 2:286577/1‑128 (MQ=255) cATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATAc < 1:286577/128‑1 (MQ=255) ttAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAgg > 4:202881/1‑105 (MQ=255) ttAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAgg < 3:202881/105‑1 (MQ=255) aaaaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa > 2:1831798/1‑117 (MQ=255) aaaaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa < 1:1831798/117‑1 (MQ=255) aaaaTTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt > 1:617579/1‑149 (MQ=255) aaaGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc > 3:130664/1‑149 (MQ=255) aaGCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag > 4:539298/1‑90 (MQ=39) aaGCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTgg < 3:1613798/121‑1 (MQ=255) aaGCCAGTCC‑GCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCag < 3:539298/90‑1 (MQ=39) aaGCCAGTCC‑GCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTgg > 4:1613798/1‑121 (MQ=255) aGCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt < 2:1805080/135‑1 (MQ=255) aGCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt > 1:1805080/1‑135 (MQ=255) gCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc < 2:1441110/128‑1 (MQ=255) gCCAGTCCGCT‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc > 1:1441110/1‑128 (MQ=255) cAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTcc > 3:1313711/1‑149 (MQ=255) gTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCATCGAATACCTGTAACGCTTGGTaa > 1:324215/1‑118 (MQ=255) gTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTaa < 2:324215/118‑1 (MQ=255) gTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGtt > 4:115416/1‑145 (MQ=255) gTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGtt < 3:115416/145‑1 (MQ=255) aGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTg < 4:130664/149‑1 (MQ=255) | TTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGC‑GGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTG > NC_000913/4612186‑4612477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |