Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,213 G→T 7.6% intergenic (+127/+20) trpR → / ← yjjX transcriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,2130GT 7.6% 368.5 / 17.2 105intergenic (+127/+20)trpR/yjjXtranscriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base G (50/47);  new base T (4/4);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 108 total aligned reads displayed.

GTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATA  >  NC_000913/4633066‑4633359
                                                                                                                                                   |                                                                                                                                                  
  gtTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCa                                                                                                                                                 <  3:246447/149‑1 (MQ=255)
  gtTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCa                                                                                                                                                 <  1:263381/149‑1 (MQ=255)
    tGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGc                                                                                                                                               <  2:528245/149‑1 (MQ=255)
                   gattTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                >  3:1452488/1‑149 (MQ=255)
                             gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       <  3:1029052/132‑6 (MQ=255)
                             gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       >  4:1029052/1‑127 (MQ=255)
                                 gATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCAtt                                                                                                                  >  1:949864/1‑149 (MQ=255)
                                  aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                                                                             >  1:1128923/1‑121 (MQ=255)
                                  aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                                                                             <  2:1128923/121‑1 (MQ=255)
                                   tAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                >  3:298242/1‑149 (MQ=255)
                                    aaGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       <  3:1861880/125‑6 (MQ=255)
                                    aaGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       >  4:1861880/1‑120 (MQ=255)
                                            gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTctta                                                                                                                                         >  3:1126785/1‑112 (MQ=255)
                                            gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATacac                                                                                                                        <  3:1656752/132‑1 (MQ=255)
                                            gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATacac                                                                                                                        >  4:1656752/1‑132 (MQ=255)
                                            gcgcATCAGGCATCGTGCACCGAATGCCGGATGCAGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTctta                                                                                                                                         <  4:1126785/115‑4 (MQ=37)
                                                aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTc                                                                                                                                            <  1:293986/108‑1 (MQ=255)
                                                aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTc                                                                                                                                            >  2:293986/1‑108 (MQ=255)
                                                aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTaa                                                                                                                             >  4:175771/1‑123 (MQ=255)
                                                aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTaa                                                                                                                             <  3:175771/123‑1 (MQ=255)
                                                 tCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACgg                                                                                                                      <  3:265009/129‑1 (MQ=255)
                                                 tCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACgg                                                                                                                      >  4:265009/1‑129 (MQ=255)
                                                    ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCa                                                                                                                    <  1:1508366/128‑1 (MQ=255)
                                                    ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCa                                                                                                                    >  2:1508366/1‑128 (MQ=255)
                                                    ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc                                                                                               >  2:567775/1‑149 (MQ=255)
                                                       aTCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAgg                                                                                            >  1:960409/1‑149 (MQ=255)
                                                         cGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGAt                                                                                          <  2:949864/149‑1 (MQ=255)
                                                             cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCAcc                                                                                      <  2:960409/149‑1 (MQ=255)
                                                                cGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGcc                                                                                   >  3:80360/1‑149 (MQ=255)
                                                                  aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  1:1770362/1‑148 (MQ=255)
                                                                  aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  2:1770362/148‑1 (MQ=255)
                                                                  aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTg                                                                                 <  4:80360/149‑1 (MQ=255)
                                                                  aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTg                                                                                 <  4:395528/149‑1 (MQ=255)
                                                                          ataaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           >  4:916977/5‑147 (MQ=255)
                                                                          aTGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGa                                                                         <  1:567775/149‑1 (MQ=255)
                                                                           taaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCa                                                                                              <  1:1666040/124‑1 (MQ=39)
                                                                              ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATg                                                                                                               <  2:869712/107‑1 (MQ=255)
                                                                              ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATg                                                                                                               >  1:869712/1‑107 (MQ=255)
                                                                               gcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTg                                                                    <  4:1452488/149‑1 (MQ=255)
                                                                                  tGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  2:1565009/132‑1 (MQ=255)
                                                                                  tGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  1:1565009/1‑132 (MQ=255)
                                                                                     acgccgcaTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              <  3:1878417/142‑1 (MQ=255)
                                                                                      cGCCTTATCCGTCCTACAAATACACGTAATTTCAATATGTATGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgag                                                             >  2:571047/1‑149 (MQ=255)
                                                                                         cgcaTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtga                                                          <  3:962968/146‑1 (MQ=255)
                                                                                         cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               >  2:1599978/1‑80 (MQ=255)
                                                                                         cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               <  1:1599978/80‑1 (MQ=255)
                                                                                         cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtga                                                          >  3:1029744/1‑149 (MQ=255)
                                                                                         cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              >  2:33892/1‑145 (MQ=255)
                                                                                         cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              <  1:33892/145‑1 (MQ=255)
                                                                                          ttATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               <  1:1179956/79‑1 (MQ=255)
                                                                                          ttATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               >  2:1179956/1‑79 (MQ=255)
                                                                                            aTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAg                                                                                             >  4:332082/1‑111 (MQ=255)
                                                                                            aTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAg                                                                                             <  3:332082/111‑1 (MQ=255)
                                                                                             tCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGttt                                                      >  3:601905/1‑149 (MQ=255)
                                                                                             tCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGttt                                                      >  4:674693/1‑149 (MQ=255)
                                                                                                  ccTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCcgg                                                 <  1:571047/149‑1 (MQ=255)
                                                                                                   cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                >  4:416712/1‑85 (MQ=255)
                                                                                                   cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                <  3:416712/85‑1 (MQ=255)
                                                                                                   cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGa                                                                                                        <  3:1343798/93‑1 (MQ=255)
                                                                                                   cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGa                                                                                                        >  4:1343798/1‑93 (MQ=255)
                                                                                                    tACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCcggcg                                               >  2:1812881/1‑149 (MQ=255)
                                                                                                      cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGt                                             >  4:290750/1‑149 (MQ=255)
                                                                                                             ccGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACg                                      >  1:1397550/1‑149 (MQ=255)
                                                                                                              cGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGc                                     <  3:674693/149‑1 (MQ=255)
                                                                                                              cGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGc                                     <  4:601905/149‑1 (MQ=255)
                                                                                                               gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           <  2:171585/78‑1 (MQ=255)
                                                                                                               gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           <  4:1724188/78‑1 (MQ=255)
                                                                                                               gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           >  3:1724188/1‑78 (MQ=255)
                                                                                                               gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           >  1:171585/1‑78 (MQ=255)
                                                                                                                    ttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                <  4:1283115/52‑1 (MQ=255)
                                                                                                                    ttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                >  3:1283115/1‑52 (MQ=255)
                                                                                                                         tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtgag                                                         <  4:1886154/118‑1 (MQ=255)
                                                                                                                         tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtgag                                                         >  3:1886154/1‑118 (MQ=255)
                                                                                                                         tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGcc                             <  3:978325/146‑1 (MQ=255)
                                                                                                                            gTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCccttcc                       >  4:663232/1‑149 (MQ=255)
                                                                                                                             tttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGAt                                                                            <  1:1333240/95‑1 (MQ=255)
                                                                                                                              ttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          <  2:1562726/128‑1 (MQ=255)
                                                                                                                              ttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          >  1:1562726/1‑128 (MQ=255)
                                                                                                                                ggTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTAc                   <  3:663232/149‑1 (MQ=255)
                                                                                                                                   aGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGAc                >  3:34766/1‑149 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  4:1388721/1‑81 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  3:1388721/81‑1 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTg                                                                              >  1:398253/1‑85 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          <  2:1523691/121‑1 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          >  1:1523691/1‑121 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCa              >  3:1501651/1‑149 (MQ=255)
                                                                                                                                     gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCa              >  3:426900/1‑149 (MQ=255)
                                                                                                                                      cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGa                 <  3:784553/145‑1 (MQ=255)
                                                                                                                                      cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGa                 >  4:784553/1‑145 (MQ=255)
                                                                                                                                       aTGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACTACCAAt            >  1:19257/1‑149 (MQ=255)
                                                                                                                                       aTGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAAt            >  1:1380916/1‑149 (MQ=255)
                                                                                                                                           tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca        >  2:1875816/1‑149 (MQ=255)
                                                                                                                                           tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca        >  4:97254/1‑149 (MQ=255)
                                                                                                                                            aaGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcat       <  3:97254/149‑1 (MQ=255)
                                                                                                                                            aaGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcat       <  2:1380916/149‑1 (MQ=255)
                                                                                                                                             aGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcatc      >  4:1254331/1‑149 (MQ=255)
                                                                                                                                               aCGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           >  4:514520/1‑78 (MQ=255)
                                                                                                                                               aCGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           <  3:514520/78‑1 (MQ=255)
                                                                                                                                                cgcgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAAt   <  4:426900/149‑1 (MQ=255)
                                                                                                                                                 gcgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATa  <  2:1397550/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATA  >  NC_000913/4633066‑4633359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: