Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,216 A→C 7.6% intergenic (+130/+17) trpR → / ← yjjX transcriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,2160AC 7.6% 372.3 / 19.0 105intergenic (+130/+17)trpR/yjjXtranscriptional repressor, tryptophan‑binding/non‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base A (50/47);  new base C (4/4);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 107 total aligned reads displayed.

GTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATA  >  NC_000913/4633068‑4633359
                                                                                                                                                    |                                                                                                                                               
  tGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGc                                                                                                                                               <  2:528245/149‑1 (MQ=255)
                 gattTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                >  3:1452488/1‑149 (MQ=255)
                           gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       <  3:1029052/132‑6 (MQ=255)
                           gCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       >  4:1029052/1‑127 (MQ=255)
                               gATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCAtt                                                                                                                  >  1:949864/1‑149 (MQ=255)
                                aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                                                                             >  1:1128923/1‑121 (MQ=255)
                                aTAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGt                                                                                                                                             <  2:1128923/121‑1 (MQ=255)
                                 tAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                >  3:298242/1‑149 (MQ=255)
                                  aaGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       >  4:1861880/1‑120 (MQ=255)
                                  aaGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTcttatc                                                                                                                                       <  3:1861880/125‑6 (MQ=255)
                                      cGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           <  4:298242/149‑1 (MQ=255)
                                          gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTctta                                                                                                                                         >  3:1126785/1‑112 (MQ=255)
                                          gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATacac                                                                                                                        <  3:1656752/132‑1 (MQ=255)
                                          gcgcATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATacac                                                                                                                        >  4:1656752/1‑132 (MQ=255)
                                          gcgcATCAGGCATCGTGCACCGAATGCCGGATGCAGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTctta                                                                                                                                         <  4:1126785/115‑4 (MQ=37)
                                              aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTc                                                                                                                                            >  2:293986/1‑108 (MQ=255)
                                              aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCTGCCGCGTc                                                                                                                                            <  1:293986/108‑1 (MQ=255)
                                              aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTaa                                                                                                                             <  3:175771/123‑1 (MQ=255)
                                              aTCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTaa                                                                                                                             >  4:175771/1‑123 (MQ=255)
                                               tCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACgg                                                                                                                      <  3:265009/129‑1 (MQ=255)
                                                  ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCa                                                                                                                    <  1:1508366/128‑1 (MQ=255)
                                                  ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCa                                                                                                                    >  2:1508366/1‑128 (MQ=255)
                                                  ggCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGcc                                                                                               >  2:567775/1‑149 (MQ=255)
                                                     aTCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAgg                                                                                            >  1:960409/1‑149 (MQ=255)
                                                           cACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCAcc                                                                                      <  2:960409/149‑1 (MQ=255)
                                                              cGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGcc                                                                                   >  3:395528/1‑149 (MQ=255)
                                                              cGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGcc                                                                                   >  3:80360/1‑149 (MQ=255)
                                                                aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  1:1770362/1‑148 (MQ=255)
                                                                aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  2:1770362/148‑1 (MQ=255)
                                                                aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTg                                                                                 <  4:395528/149‑1 (MQ=255)
                                                                aaTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTg                                                                                 <  4:80360/149‑1 (MQ=255)
                                                                        ataaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           >  4:916977/5‑147 (MQ=255)
                                                                        aTGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGa                                                                         <  1:567775/149‑1 (MQ=255)
                                                                         taaggcgTTCACGCCGCATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCa                                                                                              <  1:1666040/124‑1 (MQ=39)
                                                                            ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATg                                                                                                               >  1:869712/1‑107 (MQ=255)
                                                                            ggcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATg                                                                                                               <  2:869712/107‑1 (MQ=255)
                                                                             gcgTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTg                                                                    <  4:1452488/149‑1 (MQ=255)
                                                                                tGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  1:1565009/1‑132 (MQ=255)
                                                                                tGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  2:1565009/132‑1 (MQ=255)
                                                                                   acgccgcaTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              <  3:1878417/142‑1 (MQ=255)
                                                                                    cGCCTTATCCGTCCTACAAATACACGTAATTTCAATATGTATGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgag                                                             >  2:571047/1‑149 (MQ=255)
                                                                                       cgcaTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtga                                                          <  3:962968/146‑1 (MQ=255)
                                                                                       cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               >  2:1599978/1‑80 (MQ=255)
                                                                                       cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               <  1:1599978/80‑1 (MQ=255)
                                                                                       cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtga                                                          >  3:1029744/1‑149 (MQ=255)
                                                                                       cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              >  2:33892/1‑145 (MQ=255)
                                                                                       cTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCga                                                              <  1:33892/145‑1 (MQ=255)
                                                                                        ttATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               <  1:1179956/79‑1 (MQ=255)
                                                                                        ttATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCtt                                                                                                                               >  2:1179956/1‑79 (MQ=255)
                                                                                          aTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAg                                                                                             >  4:332082/1‑111 (MQ=255)
                                                                                          aTCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAg                                                                                             <  3:332082/111‑1 (MQ=255)
                                                                                           tCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGttt                                                      >  4:674693/1‑149 (MQ=255)
                                                                                           tCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGttt                                                      >  3:601905/1‑149 (MQ=255)
                                                                                                ccTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCcgg                                                 <  1:571047/149‑1 (MQ=255)
                                                                                                 cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                <  3:416712/85‑1 (MQ=255)
                                                                                                 cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTAt                                                                                                                >  4:416712/1‑85 (MQ=255)
                                                                                                 cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGa                                                                                                        <  3:1343798/93‑1 (MQ=255)
                                                                                                 cTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGa                                                                                                        >  4:1343798/1‑93 (MQ=255)
                                                                                                  tACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCcggcg                                               >  2:1812881/1‑149 (MQ=255)
                                                                                                    cAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGt                                             >  4:290750/1‑149 (MQ=255)
                                                                                                       aTACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          <  4:1029744/149‑1 (MQ=255)
                                                                                                           ccGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACg                                      >  1:1397550/1‑149 (MQ=255)
                                                                                                            cGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGc                                     <  4:601905/149‑1 (MQ=255)
                                                                                                            cGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGc                                     <  3:674693/149‑1 (MQ=255)
                                                                                                             gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           <  4:1724188/78‑1 (MQ=255)
                                                                                                             gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           >  1:171585/1‑78 (MQ=255)
                                                                                                             gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           >  3:1724188/1‑78 (MQ=255)
                                                                                                             gTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAAc                                                                                                           <  2:171585/78‑1 (MQ=255)
                                                                                                                  ttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                <  4:1283115/52‑1 (MQ=255)
                                                                                                                  ttCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCt                                                                                                                                >  3:1283115/1‑52 (MQ=255)
                                                                                                                       tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtgag                                                         >  3:1886154/1‑118 (MQ=255)
                                                                                                                       tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCgagtgag                                                         <  4:1886154/118‑1 (MQ=255)
                                                                                                                       tatGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGcc                             <  3:978325/146‑1 (MQ=255)
                                                                                                                          gTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCccttcc                       >  4:663232/1‑149 (MQ=255)
                                                                                                                           tttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGAt                                                                            >  2:1333240/1‑95 (MQ=255)
                                                                                                                           tttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGAt                                                                            <  1:1333240/95‑1 (MQ=255)
                                                                                                                            ttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          <  2:1562726/128‑1 (MQ=255)
                                                                                                                            ttGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          >  1:1562726/1‑128 (MQ=255)
                                                                                                                              ggTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTAc                   <  3:663232/149‑1 (MQ=255)
                                                                                                                                 aGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGAc                >  3:34766/1‑149 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  <  3:1388721/81‑1 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCt                                                                                  >  4:1388721/1‑81 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTg                                                                              >  1:398253/1‑85 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTg                                                                              <  2:398253/85‑1 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTaaa                                          >  1:1523691/1‑121 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCa              >  3:1501651/1‑149 (MQ=255)
                                                                                                                                   gCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCa              >  3:426900/1‑149 (MQ=255)
                                                                                                                                    cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGa                 <  3:784553/145‑1 (MQ=255)
                                                                                                                                    cATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGa                 >  4:784553/1‑145 (MQ=255)
                                                                                                                                     aTGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACTACCAAt            >  1:19257/1‑149 (MQ=255)
                                                                                                                                     aTGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAAt            >  1:1380916/1‑149 (MQ=255)
                                                                                                                                         tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca        >  4:97254/1‑149 (MQ=255)
                                                                                                                                         tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca        <  1:1875816/149‑1 (MQ=255)
                                                                                                                                         tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca        >  2:1875816/1‑149 (MQ=255)
                                                                                                                                          aaGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcat       <  2:1380916/149‑1 (MQ=255)
                                                                                                                                           aGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcatc      >  4:1254331/1‑149 (MQ=255)
                                                                                                                                             aCGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           >  4:514520/1‑78 (MQ=255)
                                                                                                                                             aCGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATa                                                                           <  3:514520/78‑1 (MQ=255)
                                                                                                                                              cgcgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAAt   <  4:426900/149‑1 (MQ=255)
                                                                                                                                               gcgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATa  <  2:1397550/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTTGCTGAAAAGCGATTGATTTTGTAGGCCTGATAAGACGTGGCGCATCAGGCATCGTGCACCGAATGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATACCCGTAATTTCAATATGTTTGGTAGGCATGATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATA  >  NC_000913/4633068‑4633359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: