Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 585,863 T→C 10.7% intergenic (‑230/‑194) ompT ← / → pauD DLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913585,8630TC10.7% 365.9 / 26.3 121intergenic (‑230/‑194)ompT/pauDDLP12 prophage; outer membrane protease VII; outer membrane protein 3b/tRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base T (56/52);  new base C (7/6);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCG  >  NC_000913/585715‑586010
                                                                                                                                                    |                                                                                                                                                   
aCTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCtt                                                                                                                                                     >  3:321814/1‑149 (MQ=255)
    tttATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTaga                                                                                                                                                 <  4:321814/149‑1 (MQ=255)
         atTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCat                                                                                                                                            <  1:454011/149‑1 (MQ=255)
               tttAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGc                                                                                                                                      >  2:914586/1‑149 (MQ=255)
               tttAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCatg                                                                                                                                           <  1:579895/144‑2 (MQ=255)
               tttAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCatg                                                                                                                                           >  2:579895/1‑143 (MQ=255)
                      ttGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATa                                                                                                                               >  2:262482/1‑149 (MQ=255)
                             aTTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAg                                                                                                                        <  1:914586/149‑1 (MQ=255)
                              ttATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGc                                                                                                                             <  1:570652/143‑1 (MQ=255)
                              ttATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGc                                                                                                                             >  2:570652/1‑143 (MQ=255)
                                aTCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATa                                                                                                                               <  1:1061199/139‑1 (MQ=255)
                                aTCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATa                                                                                                                               >  2:1061199/1‑139 (MQ=255)
                                 tCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGc                                                                                                                             >  1:899564/1‑140 (MQ=255)
                                 tCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGc                                                                                                                             <  2:899564/140‑1 (MQ=255)
                                          gcTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATaa                                                                                                                                        >  2:831208/1‑120 (MQ=255)
                                          gcTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATaa                                                                                                                                        <  1:831208/120‑1 (MQ=255)
                                          gcTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAAt                                                                                                           >  2:1278133/1‑149 (MQ=255)
                                              tCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAg                                                                                                                        <  4:616387/132‑1 (MQ=255)
                                              tCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAg                                                                                                                        >  3:616387/1‑132 (MQ=255)
                                                        gTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTa                                                                                             <  1:1278133/149‑1 (MQ=255)
                                                            aaTTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtt                                                                                                  >  1:458190/1‑140 (MQ=255)
                                                            aaTTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtt                                                                                                  <  2:1240366/140‑1 (MQ=255)
                                                            aaTTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtt                                                                                                  >  1:1240366/1‑140 (MQ=255)
                                                              ttATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGc                                                                                                                                      >  3:1146753/1‑102 (MQ=255)
                                                              ttATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAg                                                                                                                        >  1:900392/1‑116 (MQ=255)
                                                              ttATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaa                                                                                       >  1:484910/1‑149 (MQ=255)
                                                                 tCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            <  1:225004/125‑1 (MQ=255)
                                                                 tCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            >  2:225004/1‑125 (MQ=255)
                                                                 tCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtt                                                                                                  <  1:655695/135‑1 (MQ=255)
                                                                 tCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtt                                                                                                  >  2:655695/1‑135 (MQ=255)
                                                                  cTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaaa                                                                                   <  2:1070758/149‑1 (MQ=255)
                                                                    gTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                                 <  1:262482/149‑1 (MQ=255)
                                                                     ttATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCatgg                                                                                                                                          >  2:1120126/1‑89 (MQ=255)
                                                                     ttATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCatgg                                                                                                                                          <  1:1120126/91‑3 (MQ=255)
                                                                     ttATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCat                                                                                                                                            >  1:1770140/1‑89 (MQ=255)
                                                                     ttATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTCTAAAGGCat                                                                                                                                            <  2:1770140/89‑1 (MQ=255)
                                                                        ttGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGa                                                                             <  2:484910/149‑1 (MQ=255)
                                                                           tttCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCa                                                                                                                            >  2:1656987/1‑99 (MQ=255)
                                                                           tttCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCa                                                                                                                            <  1:1656987/99‑1 (MQ=255)
                                                                              cATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAAc                                                                       >  2:41609/1‑149 (MQ=255)
                                                                                    tCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGac                                                                 >  3:512120/1‑149 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            <  4:361553/102‑1 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            >  3:361553/1‑102 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAAt                                                                                                           >  1:1818966/1‑103 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAAt                                                                                                           <  2:1818966/103‑1 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                <  3:895/130‑1 (MQ=255)
                                                                                        ttttACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                >  4:895/1‑130 (MQ=255)
                                                                                             cTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGc                                                        <  4:512120/149‑1 (MQ=255)
                                                                                                tgtgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGc                                                                                                                                      <  3:816165/68‑1 (MQ=255)
                                                                                                tgtgGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTc                                                     >  1:329479/1‑149 (MQ=255)
                                                                                                    gTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCttt                                                                                                                                                    <  2:1771065/50‑1 (MQ=255)
                                                                                                    gTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCttt                                                                                                                                                    >  1:1771065/1‑50 (MQ=255)
                                                                                                     tttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTg                                                                     <  3:539633/128‑1 (MQ=255)
                                                                                                     tttttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAAt                                                >  3:1343331/1‑149 (MQ=255)
                                                                                                        ttttATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGc                                             <  2:521005/149‑1 (MQ=255)
                                                                                                                ttttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACt                                                                                                       <  3:991915/83‑1 (MQ=255)
                                                                                                                ttttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaaa                                                                                   <  2:1544720/103‑1 (MQ=255)
                                                                                                                ttttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaaa                                                                                   >  1:1544720/1‑103 (MQ=255)
                                                                                                                ttttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAgg                                     >  3:1012803/1‑149 (MQ=255)
                                                                                                                ttttGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAgg                                     >  2:957242/1‑149 (MQ=255)
                                                                                                                       gtgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTga                                                                    <  1:1230518/111‑1 (MQ=255)
                                                                                                                       gtgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTga                                                                    >  2:1230518/1‑111 (MQ=255)
                                                                                                                        tgCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACaaaaaa                                                                                   >  1:219473/1‑95 (MQ=255)
                                                                                                                         gCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAggg                                                                              <  3:1022843/99‑1 (MQ=255)
                                                                                                                         gCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAggg                                                                              >  4:1022843/1‑99 (MQ=255)
                                                                                                                           ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGGGAAAATAGCATGAGGt                                                                                                                      >  4:1104282/1‑57 (MQ=255)
                                                                                                                           ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGt                                                                                                                      <  3:1104282/57‑1 (MQ=255)
                                                                                                                           ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            <  4:835496/67‑1 (MQ=255)
                                                                                                                           ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCaa                                                                                                            >  3:835496/1‑67 (MQ=255)
                                                                                                                           ttttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGttttt                                                                                               >  2:203284/1‑80 (MQ=255)
                                                                                                                            tttATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGtttttt                                                                                              <  4:368373/80‑1 (MQ=255)
                                                                                                                               aTCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                      <  4:1343331/149‑1 (MQ=255)
                                                                                                                               aTCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                      <  1:585504/149‑1 (MQ=255)
                                                                                                                                  tATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGc                                    >  2:1373303/1‑132 (MQ=255)
                                                                                                                                  tATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGc                                    <  1:1373303/132‑1 (MQ=255)
                                                                                                                                   aTTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                >  2:1119613/1‑87 (MQ=255)
                                                                                                                                   aTTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                <  1:1119613/87‑1 (MQ=255)
                                                                                                                                   aTTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                >  1:1035844/1‑87 (MQ=255)
                                                                                                                                    tttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATc                                                                                                                 >  3:565058/1‑53 (MQ=255)
                                                                                                                                    tttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATc                                                                                                                 <  4:565058/53‑1 (MQ=255)
                                                                                                                                    tttAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAg                 >  4:1380998/1‑149 (MQ=255)
                                                                                                                                       aaGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTgg              <  2:1676573/149‑1 (MQ=255)
                                                                                                                                          ttataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                                 <  4:1883007/107‑1 (MQ=255)
                                                                                                                                          ttataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCaa                                                 >  3:1883007/5‑111 (MQ=255)
                                                                                                                                           tataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                                 <  2:350616/75‑1 (MQ=255)
                                                                                                                                            ataTGCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgt         >  1:1476675/3‑149 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                >  4:1259491/1‑76 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAagag                                                                                <  3:1259491/76‑1 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAaga                                                                                 >  4:647166/1‑75 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCt                                                      >  3:735332/1‑102 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCt                                                      >  1:1696514/1‑102 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCt                                                      <  4:735332/102‑1 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                      >  2:1251692/1‑134 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGa                      <  1:1251692/134‑1 (MQ=255)
                                                                                                                                              aTGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACgtgt       <  1:41609/149‑1 (MQ=255)
                                                                                                                                               tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCACTTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTga                                                                    <  4:1101379/87‑1 (MQ=255)
                                                                                                                                               tGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGtgtg      >  4:859558/1‑149 (MQ=255)
                                                                                                                                                gCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCaa                                       >  4:991211/1‑115 (MQ=255)
                                                                                                                                                gCCTCTAAAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTACAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGa     <  2:741248/149‑1 (MQ=255)
                                                                                                                                                   tttAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAgcg  >  3:22767/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACTGTTTATATTTTGTTTAGTATTGAATGATTATCACAATGCGCTATCTGTTTTTGGTTTAATTATCTGTTATTGTTTCATATTTCGGTTTTACTGTGTGGTTTTTTTATGCTTTTGTGGTGCTTTTATCTATTTAAGTGCCATGCCTTTAGAGGCATATAAGCGAAAATAGCATGAGGTTTATCCTCAATTACTATGTTTTTTAGTACAAAAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCG  >  NC_000913/585715‑586010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: