Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 594,946 C→A 8.1% Q166H (CAG→CAT) ‡ cusR ← response regulator in two‑component regulatory system with CusS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913594,9460CA 8.1% 569.3 / 30.5 161Q166H (CAG→CAT) ‡cusRresponse regulator in two‑component regulatory system with CusS
Reads supporting (aligned to +/- strand):  ref base C (73/75);  new base A (6/7);  total (79/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 169 total aligned reads displayed.

CGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGCC  >  NC_000913/594804‑595094
                                                                                                                                              |                                                                                                                                                    
cGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATgg                                                                                                                                                <  1:1133241/149‑1 (MQ=255)
   tCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGa                                                                                                                                                   >  4:1113351/1‑143 (MQ=255)
   tCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCga                                                                                                                                             >  3:836923/1‑149 (MQ=255)
   tCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCga                                                                                                                                             >  2:1031460/1‑149 (MQ=255)
    cTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                            >  3:1164369/1‑149 (MQ=255)
    cTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            <  3:1387021/149‑1 (MQ=255)
     tGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                           >  1:497913/1‑149 (MQ=255)
      gAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt                                                                                                                                          <  1:1269753/149‑2 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                       >  1:1750684/1‑149 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                       <  1:1031460/149‑1 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            <  4:300809/144‑1 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            >  3:300809/1‑144 (MQ=255)
           aGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                     <  1:1132381/149‑1 (MQ=255)
           aGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                     <  1:1609396/149‑1 (MQ=255)
            gCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAAc                                                                                                                                    >  2:1077204/1‑149 (MQ=255)
             cTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCAAGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            >  4:1516704/1‑140 (MQ=255)
                  gCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCca                                                                                                                                >  2:1414616/1‑147 (MQ=255)
                    tCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcag                                                                                                                            >  2:414592/1‑149 (MQ=255)
                     cAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCCGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                           <  4:1428276/149‑1 (MQ=255)
                        aGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTa                                                                                                                        >  4:752444/1‑149 (MQ=255)
                           cGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAAc                                                                                                                     <  3:1001331/149‑1 (MQ=255)
                             ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt                                                                                                                                          >  1:178786/1‑125 (MQ=255)
                              tGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActct                                                                                                                  <  1:524169/149‑1 (MQ=255)
                              tGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActct                                                                                                                  <  4:836923/149‑1 (MQ=255)
                               gTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            >  3:1786671/1‑122 (MQ=255)
                               gTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                            <  4:1786671/122‑1 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGa                                                                                                                                                   >  4:1719231/1‑114 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGa                                                                                                                                                   <  3:1719231/114‑1 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                           >  4:915860/1‑138 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCttt                                                                                                                >  3:720001/1‑149 (MQ=255)
                                  gATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTAc                                                                                                              >  3:457829/1‑149 (MQ=255)
                                    ttttGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                            >  3:769668/1‑149 (MQ=255)
                                    ttttGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                            >  2:814220/1‑149 (MQ=255)
                                         ccGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaa                                                                                                       >  4:1281868/1‑149 (MQ=255)
                                               aGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 <  4:720001/149‑1 (MQ=255)
                                                 ccGCTTCACCGCCACATCAATAGCATTGGTATCGCAGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAAc                                                                                                                     >  4:425248/1‑127 (MQ=255)
                                                   gCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATgcgc                                                                                             <  4:53073/149‑1 (MQ=255)
                                                          cGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGc                                                                                      >  1:298093/1‑149 (MQ=255)
                                                            ccACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTg                                                                                    >  3:928045/1‑149 (MQ=255)
                                                                aTCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCggg                                                                                >  4:106995/1‑149 (MQ=255)
                                                                 tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                  <  4:344434/98‑1 (MQ=255)
                                                                 tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                  >  3:344434/1‑98 (MQ=255)
                                                                 tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                           >  1:816295/1‑105 (MQ=255)
                                                                   aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                           >  1:1273211/1‑87 (MQ=255)
                                                                   aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGa                                                                             >  4:185820/1‑149 (MQ=255)
                                                                    aTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGccc                                                                                                                                                      <  4:1164283/75‑1 (MQ=255)
                                                                    aTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGccc                                                                                                                                                      >  3:1164283/1‑75 (MQ=255)
                                                                     tAGCATTGGTATCGCTGTCAATATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACt                                                                           <  1:781098/149‑1 (MQ=255)
                                                                     tAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACt                                                                           >  4:653332/1‑149 (MQ=255)
                                                                        cATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 >  2:476402/1‑124 (MQ=255)
                                                                         aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAAc                                                                                                                                    <  1:108997/88‑1 (MQ=255)
                                                                         aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                       <  4:297692/149‑1 (MQ=255)
                                                                          ttGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa                                                                                                      >  1:664559/1‑117 (MQ=255)
                                                                          ttGGTATCGCTGTCAAAAATCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACATTGCg                                                                      >  2:1456126/1‑149 (MQ=255)
                                                                             gTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCg                                                                      >  2:1761290/1‑146 (MQ=255)
                                                                               aTCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGa                                                                 <  4:457829/149‑1 (MQ=255)
                                                                                tCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg                                                                                 <  4:1741159/132‑1 (MQ=255)
                                                                                     gTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCt                                                                   >  1:830509/1‑141 (MQ=255)
                                                                                     gTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCt                                                                   <  2:830509/141‑1 (MQ=255)
                                                                                     gTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGAt                                                           >  3:1319834/1‑149 (MQ=255)
                                                                                       cAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCg                                                         <  2:298093/149‑1 (MQ=255)
                                                                                        aaaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGt                                                                               >  3:647863/1‑126 (MQ=255)
                                                                                        aaaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGa                                                        >  3:1043398/1‑149 (MQ=255)
                                                                                           aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCtt                                                                                                                 >  2:109919/1‑89 (MQ=255)
                                                                                           aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCa                                                     <  3:4640/149‑1 (MQ=255)
                                                                                           aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCa                                                     <  2:1750684/149‑1 (MQ=255)
                                                                                               aTGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGAc                                                                            <  2:560916/122‑1 (MQ=255)
                                                                                               aTGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGAc                                                                            >  1:560916/1‑122 (MQ=255)
                                                                                                 gTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAAt                                               <  3:653332/149‑1 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGc                                                                                   >  3:1784725/1‑110 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATc                                                          >  3:1618755/1‑135 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATc                                                          <  4:1618755/135‑1 (MQ=255)
                                                                                                       tACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCaa                                         <  4:1319834/149‑1 (MQ=255)
                                                                                                                ggCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTg                                                                                                   <  1:207701/82‑1 (MQ=255)
                                                                                                                ggCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTg                                                                                                   >  2:207701/1‑82 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 <  1:1177279/81‑1 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 >  2:75766/1‑81 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 <  1:75766/81‑1 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                 >  2:1177279/1‑81 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGAc                             <  3:106995/149‑1 (MQ=255)
                                                                                                                     tAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGAGTTTGCGGCTGACGAGATCGATCATCCAATCGGCAACCTGGAACTGACtt                           >  3:1598481/1‑149 (MQ=255)
                                                                                                                       agcgagtgGGGCAGCTCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACATTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTc                         <  1:1456126/149‑1 (MQ=255)
                                                                                                                       agcgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAAt                                               <  2:581818/127‑1 (MQ=255)
                                                                                                                         cgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATgcgc                                                                                             <  3:546063/79‑1 (MQ=255)
                                                                                                                         cgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATgcgc                                                                                             >  4:546063/1‑79 (MQ=255)
                                                                                                                         cgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATc                                                   >  3:1514159/1‑121 (MQ=255)
                                                                                                                          gagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTg                                                                  <  1:461690/105‑1 (MQ=255)
                                                                                                                            gcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATaa                    >  2:702749/1‑149 (MQ=255)
                                                                                                                                  cagcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                      <  3:3963/141‑1 (MQ=255)
                                                                                                                                  cagcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                      >  4:3963/1‑141 (MQ=255)
                                                                                                                                   agcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTc                         <  3:1267155/137‑1 (MQ=255)
                                                                                                                                    ttccTTCGCCATCAGGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCg                                                         <  3:263164/101‑1 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGTGGGTGACTTTGCGGCTGa                                                                 <  4:1679362/89‑1 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGa                                                                 >  3:1679362/1‑89 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCcgc     >  1:941942/1‑149 (MQ=255)
                                                                                                                                           gCCCTGATGACGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGAc                             <  4:1248123/125‑1 (MQ=255)
                                                                                                                                            cccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCa                                                     <  2:71884/100‑1 (MQ=255)
                                                                                                                                            cccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCa                                                     >  1:71884/1‑100 (MQ=255)
                                                                                                                                             ccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                      <  1:1011165/130‑1 (MQ=255)
                                                                                                                                              cTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGcgcc  >  2:1724777/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGCC  >  NC_000913/594804‑595094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: