Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 594,950 T→G 7.9% H165P (CAT→CCT)  cusR ← response regulator in two‑component regulatory system with CusS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913594,9500TG 7.9% 580.6 / 35.4 165H165P (CAT→CCT) cusRresponse regulator in two‑component regulatory system with CusS
Reads supporting (aligned to +/- strand):  ref base T (76/76);  new base G (6/7);  total (82/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 167 total aligned reads displayed.

CGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGCCGCA  >  NC_000913/594804‑595097
                                                                                                                                                  |                                                                                                                                                   
cGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATgg                                                                                                                                                   <  1:1133241/149‑1 (MQ=255)
   tCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCga                                                                                                                                                >  2:1132381/1‑149 (MQ=255)
    cTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                               >  3:1164369/1‑149 (MQ=255)
    cTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                               <  3:1387021/149‑1 (MQ=255)
     tGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                              >  1:497913/1‑149 (MQ=255)
      gAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt                                                                                                                                             <  1:1269753/149‑2 (MQ=255)
       aaTTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaagga                                                                                                                                            >  3:297692/1‑149 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                          <  1:1031460/149‑1 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaaggaag                                                                                                                                          >  1:1750684/1‑149 (MQ=255)
         ttAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                               >  3:300809/1‑144 (MQ=255)
           aGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                        <  1:1609396/149‑1 (MQ=255)
           aGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGgaagaa                                                                                                                                        <  1:1132381/149‑1 (MQ=255)
            gCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAAc                                                                                                                                       >  2:1077204/1‑149 (MQ=255)
             cTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                               <  3:1516704/140‑1 (MQ=255)
             cTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGAAGGACTTCGCCCTGATGGCGAAGGAAGAACt                                                                                                                                      <  4:1164369/149‑1 (MQ=255)
             cTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCAAGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaa                                                                                                                                               >  4:1516704/1‑140 (MQ=255)
              ttCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                     >  3:53073/1‑149 (MQ=255)
                   cTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                               <  2:1527461/134‑1 (MQ=255)
                    tCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcag                                                                                                                               >  4:1001331/1‑149 (MQ=255)
                    tCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCcagcag                                                                                                                               >  2:414592/1‑149 (MQ=255)
                     cAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                              >  2:1749114/1‑149 (MQ=255)
                     cAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCCGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                              <  4:179455/149‑1 (MQ=255)
                      aaaGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCagag                                                                                                                             >  2:923557/1‑149 (MQ=255)
                        aGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTa                                                                                                                           >  4:752444/1‑149 (MQ=255)
                           cGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAAc                                                                                                                        <  3:1001331/149‑1 (MQ=255)
                            gTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                               >  1:523285/1‑125 (MQ=255)
                            gTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaa                                                                                                                                               <  2:523285/125‑1 (MQ=255)
                             ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt                                                                                                                                             >  1:178786/1‑125 (MQ=255)
                             ttGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCATCAGGGCgaagt                                                                                                                                             <  2:178786/126‑2 (MQ=255)
                              tGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAActct                                                                                                                     <  4:836923/149‑1 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                              <  3:915860/138‑1 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                              >  4:915860/1‑138 (MQ=255)
                                tCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCttt                                                                                                                   >  3:635854/1‑149 (MQ=255)
                                  gATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTAc                                                                                                                 >  3:457829/1‑149 (MQ=255)
                                    ttttGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTg                                                                                                               >  3:769668/1‑149 (MQ=255)
                                       tGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTc                                                                                                            <  3:752444/149‑1 (MQ=255)
                                        gCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCa                                                                                                           >  3:670793/1‑149 (MQ=255)
                                         ccGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaa                                                                                                          >  4:1281868/1‑149 (MQ=255)
                                            cgcAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGt                                                                                                       <  4:431100/149‑1 (MQ=255)
                                              cAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGa                                                                                                     <  3:1061421/149‑1 (MQ=255)
                                               aGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                    <  4:720001/149‑1 (MQ=255)
                                               aGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                    <  1:814220/149‑1 (MQ=255)
                                                 ccGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAAc                                                                                                                        <  3:425248/127‑1 (MQ=255)
                                                   gCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATgcgc                                                                                                >  2:781098/1‑149 (MQ=255)
                                                   gCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATgcgc                                                                                                <  4:53073/149‑1 (MQ=255)
                                                          cGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGc                                                                                         >  1:298093/1‑149 (MQ=255)
                                                           gCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCt                                                                                        >  4:1394716/1‑149 (MQ=255)
                                                                aTCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCggg                                                                                   >  4:106995/1‑149 (MQ=255)
                                                                 tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTc                                                                                                                                     <  4:344434/98‑1 (MQ=255)
                                                                 tCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCaga                                                                                                                              <  2:816295/105‑1 (MQ=255)
                                                                   aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                              >  1:1273211/1‑87 (MQ=255)
                                                                   aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGa                                                                                >  4:185820/1‑149 (MQ=255)
                                                                   aaTAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGACGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCgaag                                                                                                                                              <  2:1273211/87‑1 (MQ=255)
                                                                     tAGCATTGGTATCGCTGTCAATATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACt                                                                              <  1:781098/149‑1 (MQ=255)
                                                                         aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAAc                                                                                                                                       <  1:108997/88‑1 (MQ=255)
                                                                         aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                          <  4:297692/149‑1 (MQ=255)
                                                                         aTTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGc                                                                          >  2:227798/1‑149 (MQ=255)
                                                                          ttGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa                                                                                                         >  1:664559/1‑117 (MQ=255)
                                                                          ttGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCaaa                                                                                                         <  2:664559/117‑1 (MQ=255)
                                                                          ttGGTATCGCTGTCAAAAATCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACATTGCg                                                                         >  2:1456126/1‑149 (MQ=255)
                                                                             gTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCg                                                                         >  2:1761290/1‑146 (MQ=255)
                                                                               aTCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGa                                                                    <  4:457829/149‑1 (MQ=255)
                                                                                tCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCgg                                                                                    >  3:1741159/1‑132 (MQ=255)
                                                                                  gCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACga                                                                 <  3:379668/149‑1 (MQ=255)
                                                                                     gTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCt                                                                      >  1:830509/1‑141 (MQ=255)
                                                                                       cAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCg                                                            <  3:1394716/149‑1 (MQ=255)
                                                                                        aaaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGt                                                                                  >  3:647863/1‑126 (MQ=255)
                                                                                        aaaaTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGt                                                                                  <  4:647863/126‑1 (MQ=255)
                                                                                           aTTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCtt                                                                                                                    >  2:109919/1‑89 (MQ=255)
                                                                                             tCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATc                                                      <  3:185820/149‑1 (MQ=255)
                                                                                               aTGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAg                                                                                                        >  4:1714061/1‑97 (MQ=255)
                                                                                               aTGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGAc                                                                               >  1:560916/1‑122 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGc                                                                                      >  3:1784725/1‑110 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGc                                                                                      <  4:1784725/110‑1 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCgg                                                                        >  2:712245/1‑124 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCgg                                                                        <  1:712245/124‑1 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATc                                                             <  4:1618755/135‑1 (MQ=255)
                                                                                                    ccATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATc                                                             >  3:1618755/1‑135 (MQ=255)
                                                                                                                ggCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTg                                                                                                      <  1:207701/82‑1 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                    <  1:1177279/81‑1 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                    <  1:75766/81‑1 (MQ=255)
                                                                                                                   gATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGAt                                                                                                    >  2:75766/1‑81 (MQ=255)
                                                                                                                     tAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGAGTTTGCGGCTGACGAGATCGATCATCCAATCGGCAACCTGGAACTGACtt                              >  3:1598481/1‑149 (MQ=255)
                                                                                                                       agcgagtgGGGCAGCTCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACATTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTc                            <  1:1456126/149‑1 (MQ=255)
                                                                                                                       agcgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAAt                                                  <  2:581818/127‑1 (MQ=255)
                                                                                                                         cgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATc                                                      <  4:1514159/121‑1 (MQ=255)
                                                                                                                         cgagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGa                          <  3:1281868/149‑1 (MQ=255)
                                                                                                                          gagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTg                                                                     <  1:461690/105‑1 (MQ=255)
                                                                                                                          gagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTg                                                                     >  2:461690/1‑105 (MQ=255)
                                                                                                                          gagcgGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                         >  4:32025/1‑149 (MQ=255)
                                                                                                                                  cagcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                         >  4:3963/1‑141 (MQ=255)
                                                                                                                                   agcaCTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTc                            >  4:1267155/1‑137 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGTGGGTGACTTTGCGGCTGa                                                                    <  4:1679362/89‑1 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGa                                                                    >  3:1679362/1‑89 (MQ=255)
                                                                                                                                           gCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCcgc        >  1:941942/1‑149 (MQ=255)
                                                                                                                                           gCCCTGATGGCAAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGag                                >  3:1248123/1‑124 (MQ=255)
                                                                                                                                             ccTGATGTCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                         >  2:1011165/1‑130 (MQ=255)
                                                                                                                                             ccTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGAt                         <  1:1011165/130‑1 (MQ=255)
                                                                                                                                               tGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGcgccg    >  4:661916/1‑149 (MQ=255)
                                                                                                                                                 aTGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGCCGCa  <  4:635854/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGTCTGAATTAGCTTCGGCTCAAAGTCGTTGTCGATTTTGCCGCGCAGCCGCTTCACCGCCACATCAATAGCATTGGTATCGCTGTCAAAATTCATGTCCCATACCTGCGAGGCGATAAGCGAGCGGGGCAGCACTTCGCCCTGATGGCGAAGGAAGAACTCCAGCAGAGTAAACTCTTTACTGGTCAAAGTGATGCGCGTGCCGCTGCGGGTGACTTTGCGGCTGACGAGATCGACCATCAAATCGGCAACCTGAAACTGACTTTCGATAATCACCGCCGCCCCGCGCCGCA  >  NC_000913/594804‑595097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: