Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 85,337 G→T 6.9% intergenic (+25/‑293) leuO → / → ilvI global transcription factor/acetolactate synthase 3 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091385,3370GT 6.9% 465.3 / 18.3 130intergenic (+25/‑293)leuO/ilvIglobal transcription factor/acetolactate synthase 3 large subunit
Reads supporting (aligned to +/- strand):  ref base G (48/73);  new base T (4/5);  total (52/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 130 total aligned reads displayed.

GTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAA  >  NC_000913/85190‑85470
                                                                                                                                                   |                                                                                                                                     
gTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGa                                                                                                                                      >  2:540904/1‑149 (MQ=255)
gTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGa                                                                                                                                      >  1:176425/1‑149 (MQ=255)
 tATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGaa                                                                                                                                     >  2:1349184/1‑149 (MQ=255)
  aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCATTTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAAt                                                                                                                                    >  4:931911/1‑149 (MQ=255)
  aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGGATAGGTCAGACATGAAt                                                                                                                                    <  2:1257633/149‑1 (MQ=255)
  aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAAt                                                                                                                                    <  1:1349184/149‑1 (MQ=255)
  aTTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAAt                                                                                                                                    >  4:407559/1‑149 (MQ=255)
     aCCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGGATGTc                                                                                                                                 <  2:86635/149‑1 (MQ=255)
      ccGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTGGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCt                                                                                                                                <  4:953694/149‑1 (MQ=255)
      ccGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCt                                                                                                                                <  4:867538/149‑1 (MQ=255)
       cGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               <  4:1381433/149‑1 (MQ=255)
         cTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGt                                                                                                                             <  2:944852/149‑1 (MQ=255)
         cTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGt                                                                                                                             <  4:1435866/149‑1 (MQ=255)
              gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTgg                                                                                                                              >  1:6658/1‑143 (MQ=255)
              gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTgg                                                                                                                              <  2:535255/143‑1 (MQ=255)
              gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTgg                                                                                                                              <  2:6658/143‑1 (MQ=255)
              gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTgg                                                                                                                              >  1:535255/1‑143 (MQ=255)
              gTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTAtt                                                                                                                        <  2:1564311/149‑1 (MQ=255)
               ttAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAAt                                                                                                                                    <  4:1177259/136‑1 (MQ=255)
               ttAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAAt                                                                                                                                    >  3:1177259/1‑136 (MQ=255)
                tAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCt                                                                                                                      <  3:1258631/149‑1 (MQ=255)
                 aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATTCATGTCTgacc                                                                                                                            >  4:1499963/1‑139 (MQ=255)
                 aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATTCATGTCTgacc                                                                                                                            <  1:1189583/142‑4 (MQ=255)
                 aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATTCATGTCTgacc                                                                                                                            <  1:1317488/142‑4 (MQ=255)
                 aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATTCATGTCTgacc                                                                                                                            >  2:1189583/1‑139 (MQ=255)
                 aaaacaaaacAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATTCATGTCTgacc                                                                                                                            <  3:1499963/142‑4 (MQ=255)
                   aacaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               >  3:831145/1‑137 (MQ=255)
                   aacaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               >  2:296528/1‑137 (MQ=255)
                   aacaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               <  1:296528/137‑1 (MQ=255)
                   aacaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCa                                                                                                                   <  3:39585/149‑1 (MQ=255)
                    acaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGa                                                                                                                                      >  4:832754/1‑129 (MQ=255)
                    acaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGc                                                                                                                    >  1:57369/1‑147 (MQ=255)
                    aaaaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGa                                                                                                                                      <  3:832754/127‑1 (MQ=255)
                     caaaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAAACCAGACATTCATGTCTGACCTATTCTGCaat                                                                                                                 <  3:1391127/149‑3 (MQ=37)
                       aaacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttt                                                                                                               >  3:1465643/1‑149 (MQ=255)
                        aacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAttttt                                                                                                              <  1:181011/149‑1 (MQ=255)
                        aacaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAttttt                                                                                                              >  1:975540/1‑149 (MQ=255)
                        aacaGCAGAACCTGTTATCTCTACTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCTAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAttttt                                                                                                              <  1:1611569/149‑1 (MQ=255)
                         acaGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttttt                                                                                                             >  3:69695/1‑149 (MQ=255)
                          cagcatAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTAGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTa                                                                                                            <  1:1643920/149‑1 (MQ=255)
                          cagcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTa                                                                                                            <  2:621575/149‑1 (MQ=255)
                           agcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAt                                                                                                           <  2:176425/149‑1 (MQ=255)
                            gcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAtt                                                                                                          <  3:784131/149‑1 (MQ=255)
                            gcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAtt                                                                                                          <  1:1088868/149‑1 (MQ=255)
                            gcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAtt                                                                                                          >  1:242891/1‑149 (MQ=255)
                            gcagAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAtt                                                                                                          <  1:540904/149‑1 (MQ=255)
                               gAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttt                                                                                                               >  4:208429/1‑141 (MQ=255)
                               gAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttt                                                                                                               <  3:208429/141‑1 (MQ=255)
                               gAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGaa                                                                                                       <  2:1200926/149‑1 (MQ=255)
                                aaCCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAAt                                                                                                      <  4:1465643/149‑1 (MQ=255)
                                 aCCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTc                                                                                                                       <  1:365382/131‑1 (MQ=255)
                                   cTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTa                                                                                                   <  4:1116956/149‑1 (MQ=255)
                                     gTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGCATGTAGa                                                                                                 <  2:1034903/149‑1 (MQ=255)
                                      ttATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               <  4:1019738/118‑1 (MQ=255)
                                      ttATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               >  3:920471/1‑118 (MQ=255)
                                      ttATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTg                                                                                                                               >  3:1019738/1‑118 (MQ=255)
                                      ttATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGaa                                                                                                <  3:407559/149‑1 (MQ=255)
                                        aTCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtt                                                                                              >  1:132312/1‑149 (MQ=255)
                                         tctctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAt                                                                                               >  4:803411/1‑147 (MQ=255)
                                          ctctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtttt                                                                                            >  4:652989/1‑149 (MQ=255)
                                          ctctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtttt                                                                                            >  2:2876/1‑149 (MQ=255)
                                           tctcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTa                                                                                           <  2:464758/149‑1 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCGAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtt                                                                                              <  4:1625175/144‑1 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGt                                                                                                                                  <  3:1841142/108‑1 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAAtt                                                                                              >  3:1625175/1‑144 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTAtt                                                                                         <  4:765287/149‑1 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTAtt                                                                                         <  4:69695/149‑1 (MQ=255)
                                             tcCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTGGCAAACGCTAGCTGATTGCAGAATAGGTCAGACATGAATGt                                                                                                                                  >  4:1841142/1‑108 (MQ=255)
                                                 ggCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGa                                                                                     <  2:975540/149‑1 (MQ=255)
                                                         gCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTCTgg                                                                             <  2:132312/149‑1 (MQ=255)
                                                           tGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTg                                                                                      <  1:221189/138‑1 (MQ=255)
                                                           tGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTg                                                                                      >  2:221189/1‑138 (MQ=255)
                                                                     gATAACGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCATAATAGGTCAGACATGAATGTCTGGTTTa                                                                                                                          <  4:1166153/92‑1 (MQ=255)
                                                                     gATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTa                                                                                                                          >  3:1166153/1‑92 (MQ=255)
                                                                     gATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTAtttt                                                                 >  3:957863/1‑149 (MQ=255)
                                                                      aTAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTa                                                                                                   >  2:244862/1‑114 (MQ=255)
                                                                      aTAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTa                                                                                                   <  1:244862/114‑1 (MQ=255)
                                                                      aTAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTa                                                                <  3:652989/149‑1 (MQ=255)
                                                                         aaGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGa                                                             <  4:957863/149‑1 (MQ=255)
                                                                           ggCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGatt                                                           <  2:1733523/149‑1 (MQ=255)
                                                                              cATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGattaat                                                        >  3:1616452/1‑149 (MQ=255)
                                                                                  aGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAAtgtgt                                                                               >  1:1145660/1‑122 (MQ=255)
                                                                                     ggatggaAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTgg                                                                             >  2:95083/1‑121 (MQ=255)
                                                                                         ggaAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATag                                             >  1:184769/1‑149 (MQ=255)
                                                                                             gagCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttttt                                                                                                             >  1:1693353/1‑81 (MQ=255)
                                                                                             gagCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCAtttttt                                                                                                             <  2:1693353/81‑1 (MQ=255)
                                                                                              agCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTAtt                                                                                                          >  2:194422/1‑83 (MQ=255)
                                                                                                 aaTTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAAt                                                                                                      >  2:315270/1‑84 (MQ=255)
                                                                                                 aaTTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAAt                                                                                                      <  1:315270/84‑1 (MQ=255)
                                                                                                       tctcAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGaa                                                                                                <  3:98315/84‑1 (MQ=255)
                                                                                                             tttGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTa                                                                                                   <  4:387362/75‑1 (MQ=255)
                                                                                                                        tAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGctctc                                                                       <  2:1055404/92‑1 (MQ=255)
                                                                                                                        tAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGctctc                                                                       >  1:1055404/1‑92 (MQ=255)
                                                                                                                           cTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGctctc                                                                       >  3:895952/1‑89 (MQ=255)
                                                                                                                           cTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGctctc                                                                       <  4:895952/89‑1 (MQ=255)
                                                                                                                                          accAGACATTCATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATa                                              >  2:1380910/3‑99 (MQ=255)
                                                                                                                                             aGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCaa  >  1:1870403/1‑140 (MQ=255)
                                                                                                                                             aGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCaa  <  2:1870403/140‑1 (MQ=255)
                                                                                                                                               aCATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTa                                                                                           >  4:560630/1‑49 (MQ=255)
                                                                                                                                               aCATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTa                                                                                           <  3:560630/49‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                     
GTATTACCGCTGCCGTTAAAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAA  >  NC_000913/85190‑85470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: