| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 645,068 | T→C | 20.0% | intergenic (‑65/+49) | rna ← / ← citT | ribonuclease I/citrate/succinate antiporter; citrate carrier |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 645,068 | 0 | T | C | 20.0% | 37.7 / 6.2 | 20 | intergenic (‑65/+49) | rna/citT | ribonuclease I/citrate/succinate antiporter; citrate carrier |
| Reads supporting (aligned to +/- strand): ref base T (9/7); new base C (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
GCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATT > NC_000913/644921‑645214 | gCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATTCTATCTATTAAATCATAAAAAAGCCCGCCAGGTg < 3:379124/149‑1 (MQ=255) tAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCACCTGGCGGGCttttt > 4:915315/1‑143 (MQ=255) tAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCACCTGGCGGGCttttt < 3:915315/146‑4 (MQ=255) aGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCACCTGGCGGGCtttt < 4:435761/143‑3 (MQ=255) aGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCACCTGGCGGGCtttt > 3:435761/1‑141 (MQ=255) gagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg < 1:1324828/135‑1 (MQ=255) gagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg > 2:1324828/1‑135 (MQ=255) atctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga > 2:616120/1‑96 (MQ=255) atctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga < 1:616120/96‑1 (MQ=255) aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATa < 2:1228515/109‑1 (MQ=255) aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATa > 1:1228515/1‑109 (MQ=255) aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCaa > 3:778884/1‑140 (MQ=255) aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCaa < 4:778884/140‑1 (MQ=255) aaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaaa > 3:30242/1‑117 (MQ=255) aaGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaaa < 4:30242/117‑1 (MQ=255) gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt < 2:1692270/149‑1 (MQ=255) gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt > 1:357000/1‑149 (MQ=255) gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt > 1:1692270/1‑149 (MQ=255) gCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt > 4:289490/1‑149 (MQ=255) ggTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtt > 3:1416198/1‑149 (MQ=255) tGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGcc > 2:1654804/1‑124 (MQ=255) tGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGcc < 1:1654804/124‑1 (MQ=255) | GCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGCGTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATT > NC_000913/644921‑645214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |