Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 654,560 A→C 7.7% intergenic (+18/+23) citB → / ← dcuC response regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,5600AC 7.7% 449.4 / 24.8 129intergenic (+18/+23)citB/dcuCresponse regulator in two‑component regulatory system with CitA/anaerobic C4‑dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base A (58/61);  new base C (5/5);  total (63/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 137 total aligned reads displayed.

GCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTT  >  NC_000913/654413‑654705
                                                                                                                                                   |                                                                                                                                                 
gcAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGaa                                                                                                                                                  <  1:1752496/149‑1 (MQ=255)
 cAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGaaa                                                                                                                                                 <  1:284076/149‑1 (MQ=255)
  aGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAGAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                >  4:1863329/1‑149 (MQ=255)
  aGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAAc                                                                                                                                                >  4:517635/1‑149 (MQ=255)
    gCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                              <  3:1384268/149‑1 (MQ=255)
        tGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCt                                                                                                                                          >  3:703024/1‑149 (MQ=255)
           ccATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCg                                                                                                                                       <  2:1785480/149‑1 (MQ=255)
           ccATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCg                                                                                                                                       <  3:1087150/149‑1 (MQ=255)
            cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                      <  3:908391/149‑1 (MQ=255)
            cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                      >  1:343347/1‑149 (MQ=255)
            cATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCgg                                                                                                                                      <  1:1006355/149‑1 (MQ=255)
              tCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGc                                                                                                                                            <  3:544264/141‑1 (MQ=255)
              tCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCa                                                                                                                                    >  4:239493/1‑149 (MQ=255)
               cAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAt                                                                                                                                   <  3:517635/149‑1 (MQ=255)
                 gCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                 <  1:1578820/149‑1 (MQ=255)
                 gCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                 >  2:1877748/1‑149 (MQ=255)
                  ccGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                                                                >  4:1626323/1‑149 (MQ=255)
                  ccGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTAc                                                                                                                                >  3:1486050/1‑149 (MQ=255)
                      accacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTAtt                                                                                                                            <  1:1553359/149‑1 (MQ=255)
                        cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGaaa                                                                                                                                                 >  4:1195479/1‑126 (MQ=255)
                        cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGaaa                                                                                                                                                 <  3:1195479/126‑1 (MQ=255)
                        cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTa                                                                                                                                 <  1:861756/142‑1 (MQ=255)
                        cacTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTa                                                                                                                          <  4:1120916/149‑1 (MQ=255)
                               aGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCt                                                                                                                   <  2:343347/149‑1 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCgg                                                                                                                                      <  4:1775386/120‑1 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGTTTCCTCCgg                                                                                                                                      >  3:1775386/1‑120 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCAtt                                                                                                                                  >  1:1280739/1‑124 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                         >  1:638078/1‑149 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                         >  3:42899/1‑149 (MQ=255)
                                         ttGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCgctg                                                                                                         >  2:1627259/1‑149 (MQ=255)
                                            aaTATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGctgctg                                                                                                      <  4:42899/149‑1 (MQ=255)
                                                   cgcCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTcc                                                                                               <  1:1627259/149‑1 (MQ=255)
                                                      cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTc                                                                                                <  1:512062/145‑1 (MQ=255)
                                                      cAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTc                                                                                                >  2:512062/1‑145 (MQ=255)
                                                                 tGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGct                                                                                 >  1:868088/1‑149 (MQ=255)
                                                                  gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGtt                                                                                                                                              <  3:1230119/87‑1 (MQ=255)
                                                                  gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCAt                                                                                      >  3:699894/1‑143 (MQ=255)
                                                                  gATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCAt                                                                                      <  4:699894/143‑1 (MQ=255)
                                                                  gATTATTGCTAAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGCAAGtt                                                                                                                                              >  4:1230119/1‑87 (MQ=255)
                                                                   attattGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGctct                                                                               <  1:1000774/149‑1 (MQ=255)
                                                                          cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                              >  2:1558029/1‑79 (MQ=255)
                                                                          cTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                              <  1:1558029/79‑1 (MQ=255)
                                                                           tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                       >  1:1378160/1‑149 (MQ=255)
                                                                           tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                       >  1:1792450/1‑149 (MQ=255)
                                                                           tGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                       <  2:1792450/149‑1 (MQ=255)
                                                                            gAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTTACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATGAGCTCTGTAGCAACg                                                                      >  2:546296/1‑149 (MQ=255)
                                                                             aaaTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGa                                                                     <  2:638078/149‑1 (MQ=255)
                                                                               aTTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATa                                                                   >  4:763721/1‑149 (MQ=255)
                                                                                ttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATaa                                                                  <  3:239493/149‑1 (MQ=255)
                                                                                ttGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATaa                                                                  <  4:728179/149‑1 (MQ=255)
                                                                                   ttCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACaa                                                               <  3:1863329/149‑1 (MQ=255)
                                                                                    tCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                        >  2:1693032/1‑139 (MQ=255)
                                                                                    tCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCaa                                                                        <  1:1693032/139‑1 (MQ=255)
                                                                                       cGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCac                                                           <  4:552229/149‑1 (MQ=255)
                                                                                          cAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaa                                                        >  3:593447/1‑149 (MQ=255)
                                                                                              gTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTa                                                                                                                          <  3:1316416/79‑1 (MQ=255)
                                                                                              gTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTa                                                                                                                          >  4:1316416/1‑79 (MQ=255)
                                                                                                  gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                              >  2:1228469/1‑55 (MQ=255)
                                                                                                  gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACtt                                                                                                                                              <  1:1228469/55‑1 (MQ=255)
                                                                                                  gCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaacaa                                                <  2:868088/149‑1 (MQ=255)
                                                                                                   cAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAg                                               <  4:593447/149‑1 (MQ=255)
                                                                                                    aGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc                                              <  1:1877748/149‑1 (MQ=255)
                                                                                                     gACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACt                                                                                                                        >  4:1818107/1‑74 (MQ=255)
                                                                                                     gACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACt                                                                                                                        <  3:1818107/74‑1 (MQ=255)
                                                                                                        cacaACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCacc                                                          <  3:1178519/133‑1 (MQ=255)
                                                                                                          caACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCAt                                                                                      <  1:572793/103‑1 (MQ=255)
                                                                                                           aaCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTa                                       >  2:999775/1‑149 (MQ=255)
                                                                                                            aCGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTAc                                      <  2:1378160/149‑1 (MQ=255)
                                                                                                               cATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGa                                   >  4:1503009/1‑149 (MQ=255)
                                                                                                               cATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGa                                   <  3:1503009/149‑1 (MQ=255)
                                                                                                                atatACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATc                                                                                     >  2:497313/1‑98 (MQ=255)
                                                                                                                atatACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATc                                                                                     <  1:497313/98‑1 (MQ=255)
                                                                                                                   taCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTgg                                                                                            <  4:501743/88‑1 (MQ=255)
                                                                                                                    aCCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCTTTCCTGGCCCCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGAACGGGTACCGAGGtcc                              >  2:851068/1‑147 (MQ=255)
                                                                                                                     ccACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCg                             >  3:541922/1‑149 (MQ=255)
                                                                                                                      cacaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaacaa                                                <  2:613793/129‑1 (MQ=255)
                                                                                                                      cacaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACcaacaa                                                >  1:613793/1‑129 (MQ=255)
                                                                                                                       acaGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATc                                                             <  2:1509762/115‑1 (MQ=255)
                                                                                                                        caGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGttt                          <  3:763721/149‑1 (MQ=255)
                                                                                                                         aGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                       >  1:1018907/1‑103 (MQ=255)
                                                                                                                         aGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAAc                                                                       <  2:1018907/103‑1 (MQ=255)
                                                                                                                           tGGGTTGCATGATAAAGCCGGAGGAAACTTGGCTCCGGCAATACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                       <  1:851068/149‑1 (MQ=255)
                                                                                                                                 aCATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc                                              >  4:102098/1‑120 (MQ=255)
                                                                                                                                 aCATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGc                                              <  3:102098/120‑1 (MQ=255)
                                                                                                                                   aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                   >  4:1301624/1‑145 (MQ=255)
                                                                                                                                   aTGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACt                   <  3:1301624/145‑1 (MQ=255)
                                                                                                                                       tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaac                                                      <  4:1854534/106‑1 (MQ=255)
                                                                                                                                       tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCaccaaac                                                      >  3:1854534/1‑106 (MQ=255)
                                                                                                                                       tAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACgg           <  4:541922/149‑1 (MQ=255)
                                                                                                                                        aaaGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGc          >  4:1187841/1‑149 (MQ=255)
                                                                                                                                          aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGt                                                                             >  2:1784205/1‑80 (MQ=255)
                                                                                                                                          aGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGt                                                                             <  1:1784205/80‑1 (MQ=255)
                                                                                                                                           gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                       >  4:1047716/1‑133 (MQ=255)
                                                                                                                                           gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                       <  4:1429167/133‑1 (MQ=255)
                                                                                                                                           gCCGGAGGCAAGTTTCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTAc                       <  3:1047716/133‑1 (MQ=255)
                                                                                                                                           gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAc                                            <  3:30086/112‑1 (MQ=255)
                                                                                                                                           gCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCAc                                            >  4:30086/1‑112 (MQ=255)
                                                                                                                                               gaggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCt   >  2:1224148/1‑149 (MQ=255)
                                                                                                                                                aggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGa                     >  3:1699420/1‑130 (MQ=255)
                                                                                                                                                aggaAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGa                     <  4:1699420/130‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCATATACCACAGTGGGTGACATGATAAAGCCGGAGGAAACTTGCCTCCGGCATTACTATTTACTTGCCTGTGACCGCTGCTGCCGTTCCTGGCACCATCAGCTCTGTAGCAACGATAACAATCACCAAACCAACAAGCACCGGTACCGAGGTGCGTTTTACGACTTCAAACGGCGAGATCTT  >  NC_000913/654413‑654705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: