Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 876,568 A→T 5.6% N412Y (AAT→TAT) ‡ yliF → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913876,5680AT 5.6% 581.7 / 18.7 160N412Y (AAT→TAT) ‡yliFputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (76/75);  new base T (4/5);  total (80/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01

Only 100 of 176 total aligned reads displayed.

GGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTA  >  NC_000913/876421‑876712
                                                                                                                                                   |                                                                                                                                                
ggTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCaaa                                                                                                                                                 <  2:1613600/149‑1 (MQ=255)
 gTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaat                                                                                                                                                >  1:1133540/1‑149 (MQ=255)
    aaGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             <  3:188981/149‑1 (MQ=255)
    aaGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             <  4:248635/149‑1 (MQ=255)
       tCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGt                                                                                                                                          >  1:919659/1‑149 (MQ=255)
          aCCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCt                                                                                                                                       >  3:1270428/1‑149 (MQ=255)
           ccGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTa                                                                                                                                      <  2:1129689/149‑1 (MQ=255)
                             aTTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaaa                                                                                                                    <  2:969400/149‑1 (MQ=255)
                                  cGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTg                                                                                                               >  1:57102/1‑149 (MQ=255)
                                   ggTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATg                                                                                                                  <  2:244161/145‑1 (MQ=255)
                                   ggTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATg                                                                                                                  >  1:244161/1‑145 (MQ=255)
                                    gTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTAcc                                                                                                                                    >  1:576040/1‑126 (MQ=255)
                                    gTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTAcc                                                                                                                                    <  2:576040/126‑1 (MQ=255)
                                     tGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCtt                                                                                                                         >  3:528886/1‑136 (MQ=255)
                                       aTTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGa                                                                                                          <  1:140142/149‑1 (MQ=255)
                                        tttATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCATTTGAATGtgc                                                                                                                                        <  4:1713771/118‑3 (MQ=255)
                                        tttATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGAt                                                                                                         >  3:29889/1‑149 (MQ=255)
                                         ttATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                                      >  1:1045277/1‑135 (MQ=255)
                                         ttATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaa                                                                                                                      <  2:1045277/135‑1 (MQ=255)
                                               gggTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTg                                                                                                               >  4:486778/1‑136 (MQ=255)
                                               gggTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGc                                                                                                  <  3:290104/149‑1 (MQ=255)
                                                  tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGa                                                                                                          <  3:1797999/138‑1 (MQ=255)
                                                  tGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGt                                                                                               <  2:919659/149‑1 (MQ=255)
                                                     aaCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             >  3:1702742/1‑100 (MQ=255)
                                                     aaCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             <  4:1702742/100‑1 (MQ=255)
                                                     aaCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             <  3:862780/100‑1 (MQ=255)
                                                     aaCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAACGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGAt                                                                                                         >  2:585589/1‑136 (MQ=255)
                                                     aaCGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAACGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGAt                                                                                                         <  1:585589/136‑1 (MQ=255)
                                                        gCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAaga                                                                                         >  4:289371/1‑149 (MQ=255)
                                                            gAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATaa                                                                                           <  3:812746/143‑1 (MQ=255)
                                                            gAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                     <  1:1564147/149‑1 (MQ=255)
                                                                agTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaaa                                                                                 <  2:1178457/149‑1 (MQ=255)
                                                                  ttGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaaaaa                                                                               >  2:627251/1‑149 (MQ=255)
                                                                   tGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAAc                                                                              >  4:1845848/1‑149 (MQ=255)
                                                                     cTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACgg                                                                            >  2:871875/1‑149 (MQ=255)
                                                                       aTCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                     >  3:584619/1‑138 (MQ=255)
                                                                        tCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATaa                                                                                           >  2:183524/1‑131 (MQ=255)
                                                                        tCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATaa                                                                                           <  1:183524/131‑1 (MQ=255)
                                                                         cGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAgg                                                                        >  4:592490/1‑149 (MQ=255)
                                                                            ttCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTg                                                                                                                        >  4:572927/1‑98 (MQ=255)
                                                                             tCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGcac                                                                    <  4:913449/149‑1 (MQ=255)
                                                                              cTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGcaca                                                                   <  3:625807/149‑1 (MQ=255)
                                                                                 aaaGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAaatgaa                                                                                                                                             <  4:311179/72‑1 (MQ=255)
                                                                                    gAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaaaac                                                                               >  3:1473624/1‑130 (MQ=255)
                                                                                    gAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGaaaaa                                                                               <  4:1473624/131‑1 (MQ=255)
                                                                                    gAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAg                                                             >  2:1509751/1‑149 (MQ=255)
                                                                                     aaaTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACgg                                                                            <  2:449390/133‑1 (MQ=255)
                                                                                     aaaTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACgg                                                                            >  1:449390/1‑133 (MQ=255)
                                                                                       aTCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                     <  4:1479707/122‑1 (MQ=255)
                                                                                          gTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCa                                                       <  3:1845848/149‑1 (MQ=255)
                                                                                           ttCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAagaga                                                                                       >  4:1247213/1‑115 (MQ=255)
                                                                                           ttCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGt                                                                                       <  3:1247213/116‑1 (MQ=255)
                                                                                              aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAaga                                                                                         >  1:233746/1‑111 (MQ=255)
                                                                                              aTTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTaa                                                   <  2:458209/149‑1 (MQ=255)
                                                                                                ttCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCt                                                                                                 >  3:335437/1‑101 (MQ=255)
                                                                                                ttCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATc                                                 <  1:17377/149‑1 (MQ=255)
                                                                                                 tCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGaaaa                                                                                                                    >  3:933420/1‑81 (MQ=255)
                                                                                                    aGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatt                                              >  1:1861032/1‑148 (MQ=255)
                                                                                                    aGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCatt                                              <  2:1861032/148‑1 (MQ=255)
                                                                                                       tGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTc                                          >  3:1492090/1‑149 (MQ=255)
                                                                                                         ggAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCa                                                       >  4:204442/1‑134 (MQ=255)
                                                                                                          gAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCa                                       <  2:1633078/149‑1 (MQ=255)
                                                                                                               aTTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCg                                  <  1:871875/149‑1 (MQ=255)
                                                                                                                ttAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCgg                                 >  1:735694/1‑149 (MQ=255)
                                                                                                                ttAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTCGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCgg                                 >  3:1524682/1‑149 (MQ=255)
                                                                                                                   aTTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTc                                                                                                                                         >  2:821480/1‑42 (MQ=255)
                                                                                                                   aTTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTc                                                                                                                                         <  1:821480/42‑1 (MQ=255)
                                                                                                                   aTTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCtt                                                                                                                         >  2:1397342/1‑58 (MQ=255)
                                                                                                                   aTTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGc                                                            <  3:240695/119‑1 (MQ=255)
                                                                                                                       aaGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGc                                                                                                  >  4:4301/1‑77 (MQ=255)
                                                                                                                         gTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGta                                                                                              <  4:11074/79‑1 (MQ=255)
                                                                                                                         gTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAAGCCGGACAACTGag                        >  4:517216/1‑148 (MQ=255)
                                                                                                                          tACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                       <  3:73557/149‑1 (MQ=255)
                                                                                                                          tACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCGTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                       <  3:517216/149‑1 (MQ=255)
                                                                                                                           aCCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAg                                                             <  4:82265/110‑1 (MQ=255)
                                                                                                                           aCCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCa                                       <  2:1814338/132‑1 (MQ=255)
                                                                                                                           aCCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCa                                       >  1:1814338/1‑132 (MQ=255)
                                                                                                                           aCCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAttt                      >  3:43366/1‑149 (MQ=255)
                                                                                                                            ccAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGa                         >  2:193064/1‑145 (MQ=255)
                                                                                                                            ccAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtttt                     <  4:1524682/149‑1 (MQ=255)
                                                                                                                            ccAGTATCGGTGTAGCACATTCAAACGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtttt                     <  4:651155/149‑1 (MQ=255)
                                                                                                                                tATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                     >  1:890154/1‑81 (MQ=255)
                                                                                                                                tATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTaa                                                                                     <  2:890154/81‑1 (MQ=255)
                                                                                                                                tATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAATGGCAGGGCACAGATTa                                                              >  3:159512/1‑104 (MQ=255)
                                                                                                                                tATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTa                                                              <  4:159512/104‑1 (MQ=255)
                                                                                                                                          ggacaTTCATTTGAATGTGCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATc                                                 <  4:781232/105‑1 (MQ=255)
                                                                                                                                          gCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGAtt                       <  1:647829/133‑1 (MQ=255)
                                                                                                                                          gCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaatt       >  2:60099/1‑149 (MQ=255)
                                                                                                                                           gacaTTCATTTGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGa                         <  4:1321386/129‑1 (MQ=255)
                                                                                                                                            acaTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAaga                                                                                         >  3:1352661/1‑65 (MQ=255)
                                                                                                                                             caTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaat    <  1:60099/149‑1 (MQ=255)
                                                                                                                                              aTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGa                                                                                                       >  4:1111680/1‑49 (MQ=255)
                                                                                                                                              aTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGaattaatt   >  1:660353/1‑149 (MQ=255)
                                                                                                                                              aTTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATCCTGGCGGATGa                                                                                                       <  3:1111680/49‑1 (MQ=255)
                                                                                                                                               ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGtat                                                                                             >  2:70251/1‑58 (MQ=255)
                                                                                                                                               ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCa                                       >  4:683269/1‑112 (MQ=255)
                                                                                                                                               ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCa                                       <  3:683269/112‑1 (MQ=255)
                                                                                                                                               ttCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAattaatta  >  2:1303660/1‑149 (MQ=255)
                                                                                                                                                 cAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa          <  1:265799/139‑1 (MQ=255)
                                                                                                                                                 cAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGa          >  2:265799/1‑139 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGTAAAGTCTACCGCTTCGGCGGTGACGAATTTGCGGTGATTTATACGGGTGGAACGCTGGAAGAGTTGCTATCGATTCTAAAAGAAATCGTTCATTTCCAGGTGGGAAGCATTAATTTAAGTACCAGTATCGGTGTAGCACATTCAAATGAATGTCCTACCGTCGAACGCTTGAAAATGCTGGCGGATGAGCGGCTGTATAAGAGTAAGAAAAACGGCAGGGCACAGATTAGCTGGCAGTAATCATTATTCGCAGGCCGGACAAATGATTTTGCCCGGCCTGAATTAATTA  >  NC_000913/876421‑876712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: