Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 926,174 A→T 5.4% intergenic (‑203/+51) serW ← / ← infA tRNA‑Ser/translation initiation factor IF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913926,1740AT 5.4% 470.5 / 12.9 129intergenic (‑203/+51)serW/infAtRNA‑Ser/translation initiation factor IF‑1
Reads supporting (aligned to +/- strand):  ref base A (62/60);  new base T (4/3);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 130 total aligned reads displayed.

GAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGC  >  NC_000913/926029‑926322
                                                                                                                                                 |                                                                                                                                                    
gAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAAc                                                                                                                                                   <  3:1509654/149‑1 (MQ=255)
         ggTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGTCGGTTTAACCGGCCtttt                                                                                                                                          <  4:507844/149‑1 (MQ=255)
                     ttttAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGTCGGTTTAACCGGCCt                                                                                                                                             <  4:1769018/134‑1 (MQ=255)
                     ttttAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGTCGGTTTAACCGGCCt                                                                                                                                             >  3:1769018/1‑134 (MQ=255)
                            gATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGc                                                                                                                       >  2:1860332/1‑149 (MQ=255)
                             aTAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATGTCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGcc                                                                                                                      >  1:51021/1‑149 (MQ=255)
                             aTAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCg                                                                                                                        >  1:374534/1‑147 (MQ=255)
                             aTAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCg                                                                                                                        <  2:374534/147‑1 (MQ=255)
                             aTAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGcc                                                                                                                      >  4:331683/1‑149 (MQ=255)
                                      gTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCttt                                                                                                                                           <  4:1434930/119‑1 (MQ=255)
                                       tttGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCgg                                                                                                            <  3:331683/149‑1 (MQ=255)
                                            ttaTACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaaa                                                                                                       >  1:821661/3‑149 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTTATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaaa                                                                                                       >  4:1328422/1‑149 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGttcttt                                                                                                                               <  2:1265349/125‑1 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGtt                                                                                                                                   <  3:129485/121‑1 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGtt                                                                                                                                   >  4:129485/1‑121 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaaa                                                                                                       >  2:1685668/1‑149 (MQ=255)
                                            tCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaaa                                                                                                       >  4:288028/1‑149 (MQ=255)
                                               tACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACaa                                                                                                    >  1:96399/1‑149 (MQ=255)
                                               tACTGTAAACAAGTTGTACAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACaa                                                                                                    >  2:1274550/1‑149 (MQ=255)
                                                  tGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTAc                                                                                                                                        >  3:1130680/1‑110 (MQ=255)
                                                  tGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTAc                                                                                                                                        <  4:1130680/110‑1 (MQ=255)
                                                    tAAACAAGTTGTGCAGTATGTCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGc                                                                                               <  2:51021/149‑1 (MQ=255)
                                                    tAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGc                                                                                               <  4:1047664/149‑1 (MQ=255)
                                                    tAAACAAGTTGTACAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGc                                                                                               <  1:1274550/149‑1 (MQ=255)
                                                      aaCAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaa                                                                                                        <  1:1659782/138‑1 (MQ=255)
                                                      aaCAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTaaa                                                                                                        >  2:1659782/1‑138 (MQ=255)
                                                      aaCAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGc                                                                                               <  4:353869/147‑1 (MQ=255)
                                                      aaCAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGc                                                                                               >  3:353869/1‑147 (MQ=255)
                                                      aaCAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGa                                                                                             >  2:1003459/1‑149 (MQ=255)
                                                         aaGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTa                                                                                          >  1:740237/1‑149 (MQ=255)
                                                           gTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACg                                                                                        >  4:725574/1‑149 (MQ=255)
                                                            ttGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGt                                                                                                                                    <  1:750036/104‑1 (MQ=255)
                                                            ttGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGt                                                                                                                                    >  2:750036/1‑104 (MQ=255)
                                                            ttGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACgg                                                                                       <  1:1003459/149‑1 (MQ=255)
                                                                 cAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGAc                                                                                  >  2:1881/1‑149 (MQ=255)
                                                                  aGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCg                                                                                                             >  1:482685/1‑121 (MQ=255)
                                                                  aGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCg                                                                                                             <  2:482685/121‑1 (MQ=255)
                                                                   gTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGAGTACGGAAGACaa                                                                                <  3:136999/149‑1 (MQ=255)
                                                                   gTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACaa                                                                                >  1:495968/1‑149 (MQ=255)
                                                                   gTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACaa                                                                                >  2:219033/1‑149 (MQ=255)
                                                                    tataTCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAAt                                                                               >  4:400696/1‑149 (MQ=255)
                                                                      tatCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGc                                                                             <  1:1860332/149‑1 (MQ=255)
                                                                       atCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGc                                                                                                              <  4:1304808/115‑1 (MQ=255)
                                                                       atCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCg                                                                            <  2:900983/149‑1 (MQ=255)
                                                                         cTACCTCGAGACAAGTTACGGACTTATACTTCCAAAGGACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTtctc                                                                                                                            <  4:1803067/99‑1 (MQ=255)
                                                                         cTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTtctc                                                                                                                            >  4:369891/1‑99 (MQ=255)
                                                                         cTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTtctc                                                                                                                            <  3:369891/99‑1 (MQ=255)
                                                                         cTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTtctc                                                                                                                            >  3:1803067/1‑99 (MQ=255)
                                                                             aTCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCttt                                                                      >  1:213379/1‑149 (MQ=255)
                                                                              tCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTTATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTg                                                                     <  3:1328422/149‑1 (MQ=255)
                                                                               cGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGc                                                                    <  2:213379/149‑1 (MQ=255)
                                                                                   aCAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAg                                                                <  3:400696/149‑1 (MQ=255)
                                                                                    cAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAgg                                                               <  3:288028/149‑1 (MQ=255)
                                                                                     aaGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt                                                                                                   >  3:828378/1‑112 (MQ=255)
                                                                                     aaGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt                                                                                                   <  4:828378/112‑1 (MQ=255)
                                                                                        ttACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACaa                                                                                <  1:1323682/128‑1 (MQ=255)
                                                                                        ttACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACaa                                                                                >  2:1323682/1‑128 (MQ=255)
                                                                                         tACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt                                                                                                   <  1:1216773/108‑1 (MQ=255)
                                                                                         tACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAAt                                                                                                   >  2:1216773/1‑108 (MQ=255)
                                                                                           cGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACg                                                        >  3:579042/1‑149 (MQ=255)
                                                                                              aCTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACgggg                                                     >  1:1716816/1‑149 (MQ=255)
                                                                                                 tataCTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCa                                                  <  2:96399/149‑1 (MQ=255)
                                                                                                 tataCTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCa                                                  >  1:700117/1‑149 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAAt                                                                               >  3:1418590/1‑115 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGc                                                                             >  2:1358985/1‑117 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGc                                                                    >  3:1408012/1‑126 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGc                                                                    <  4:1408012/126‑1 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACggg                                                      >  1:1444942/1‑140 (MQ=255)
                                                                                                      ttCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACggg                                                      <  2:1444942/140‑1 (MQ=255)
                                                                                                       tCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            >  1:1259448/1‑149 (MQ=255)
                                                                                                       tCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            <  2:1259448/149‑1 (MQ=255)
                                                                                                        ccAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAAc                                           <  2:740237/149‑1 (MQ=255)
                                                                                                         cAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGa                                                                                             <  1:465917/98‑1 (MQ=255)
                                                                                                         cAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGa                                                                                             >  2:465917/1‑98 (MQ=255)
                                                                                                         cAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACa                                          >  1:1281461/1‑149 (MQ=255)
                                                                                                            aGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTc                                       <  3:725574/149‑1 (MQ=255)
                                                                                                                   catacataTCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGt                                <  1:1881/149‑1 (MQ=255)
                                                                                                                    atacataTCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTc                               <  4:579042/149‑1 (MQ=255)
                                                                                                                            cacaAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCa                       <  2:746423/149‑1 (MQ=255)
                                                                                                                             acaAAATAAAAAGGCCGGTTAAACCGACCCTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            >  2:74472/1‑127 (MQ=255)
                                                                                                                             acaAAATAAAAAGGCCGGTTAAACCGACCCTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            <  1:74472/127‑1 (MQ=255)
                                                                                                                               aaaaTAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACa                                          <  3:1022817/127‑1 (MQ=255)
                                                                                                                               aaaaTAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACa                                          >  4:1022817/1‑127 (MQ=255)
                                                                                                                               aaaaTAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGa                    <  1:1091925/149‑1 (MQ=255)
                                                                                                                                  aTAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGc                 >  4:861518/1‑149 (MQ=255)
                                                                                                                                   tAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCAc                                     >  4:568405/1‑128 (MQ=255)
                                                                                                                                     aaaaGGTCGGTTTAACCGGCCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGccc                           <  2:550380/136‑1 (MQ=255)
                                                                                                                                     aaaaGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGa              >  3:1197165/1‑149 (MQ=255)
                                                                                                                                     aaaaGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGa              <  1:272618/149‑1 (MQ=255)
                                                                                                                                      aaaGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGAt             <  2:700117/149‑1 (MQ=255)
                                                                                                                                        aGGTCGGTTTAACCGGCCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGt           >  1:831391/1‑149 (MQ=255)
                                                                                                                                        aGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            >  4:1709013/1‑116 (MQ=255)
                                                                                                                                        aGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCaa                                            <  3:1709013/116‑1 (MQ=255)
                                                                                                                                             ggTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGttt      >  2:1070525/1‑149 (MQ=255)
                                                                                                                                              gTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTc                               <  4:1625961/123‑1 (MQ=255)
                                                                                                                                              gTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTc                               >  3:1625961/1‑123 (MQ=255)
                                                                                                                                               ttAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGttttt    <  2:1716816/149‑1 (MQ=255)
                                                                                                                                                tAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTg   <  1:1509399/149‑1 (MQ=255)
                                                                                                                                                 aaaCCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTgc  >  1:558788/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGTATATCTACATCGAGACAAGTTACGGACTTATACTTCCAAAGTACTTCATACATATCACAAAATAAAAAGGCCGGTTAAACCGACCTTTTACTCGTTCTTTCTCTTCGCCCATCAGGCGGTAAAACAATCAGCGACTACGGAAGACAATGCGGCCTTTGCTCAGGTCGTACGGGGTCAGTTCAACAGTCACTTTGTCGCCCGTCAGGATGCGGATGTAGTTTTTGC  >  NC_000913/926029‑926322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: