| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,159,336 | A→C | 8.3% | intergenic (+34/+26) | ptsG → / ← fhuE | fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,159,336 | 0 | A | C | 8.3% | 158.1 / 7.4 | 48 | intergenic (+34/+26) | ptsG/fhuE | fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base A (21/23); new base C (2/2); total (23/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01 | |||||||||||
AAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGA > NC_000913/1159189‑1159480 | aagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt < 2:793144/149‑1 (MQ=255)aagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt > 4:712847/1‑149 (MQ=255) aaaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGc < 3:1031209/149‑1 (MQ=255) aaaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGc < 3:682687/149‑1 (MQ=255) aaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt < 3:1839289/149‑1 (MQ=255) aaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt < 3:712847/149‑1 (MQ=255) tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc < 1:858207/130‑1 (MQ=255) tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc > 2:858207/1‑130 (MQ=255) tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt > 4:102045/1‑111 (MQ=255) tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt < 3:102045/111‑1 (MQ=255) gtgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc > 3:80633/1‑115 (MQ=255) gtgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc < 4:80633/115‑1 (MQ=255) ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa > 2:1096906/1‑138 (MQ=255) ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa < 1:1096906/138‑1 (MQ=255) aaTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa > 1:1317215/1‑115 (MQ=255) aaTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGaa < 2:1317215/115‑1 (MQ=255) aaTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt > 4:984719/1‑149 (MQ=255) ggatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa > 2:654449/1‑96 (MQ=255) ggatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa < 1:654449/96‑1 (MQ=255) gAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg < 4:448188/116‑1 (MQ=255) gAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcg > 3:448188/1‑116 (MQ=255) gTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt < 3:1162975/105‑1 (MQ=255) gTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtgt > 3:1419580/1‑120 (MQ=255) gTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtgt < 4:1419580/120‑1 (MQ=255) gTACATCCGTAACCACTAATCCGTAAGACGTAGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCAGGTAATGCt > 4:1162975/1‑105 (MQ=255) aTCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg > 4:1845356/1‑67 (MQ=255) aTCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg < 3:1845356/67‑1 (MQ=255) tCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg < 1:100356/111‑1 (MQ=255) tCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAGTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg > 2:100356/1‑111 (MQ=255) cACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa > 1:246845/1‑120 (MQ=255) cACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa < 2:246845/120‑1 (MQ=255) aCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTcc > 3:1355940/1‑128 (MQ=255) aCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTcc < 4:1355940/128‑1 (MQ=255) tAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt > 4:841788/1‑89 (MQ=255) tAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt < 3:841788/89‑1 (MQ=255) tAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt < 3:1723312/82‑1 (MQ=255) tAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt > 4:1723312/1‑82 (MQ=255) cTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCt < 3:984719/149‑1 (MQ=255) aGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGtt < 2:1200632/105‑1 (MQ=255) aGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGtt > 1:1200632/1‑105 (MQ=255) aGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATc < 2:220378/111‑1 (MQ=255) aGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATc > 1:220378/1‑111 (MQ=255) gCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGCTAGAACCTTCTACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACgag > 2:676259/1‑149 (MQ=255) ccAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACgaga > 3:1762895/1‑149 (MQ=255) aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtg < 4:430701/69‑1 (MQ=255) aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgtg > 3:430701/1‑69 (MQ=255) aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTccc > 2:1210459/1‑136 (MQ=255) aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTccc < 1:1210459/136‑1 (MQ=255) | AAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGA > NC_000913/1159189‑1159480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |