Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,177,647 C→A 5.0% L110I (CTT→ATT)  lolE → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,177,6470CA 5.0% 435.4 / 10.3 119L110I (CTT→ATT) lolElipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base C (56/57);  new base A (3/3);  total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 119 total aligned reads displayed.

GTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGC  >  NC_000913/1177503‑1177789
                                                                                                                                                |                                                                                                                                              
gtggtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                                                                            <  3:179002/149‑1 (MQ=255)
 tggtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcgc                                                                                                                                           <  1:145553/149‑1 (MQ=255)
 tggtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcgc                                                                                                                                           <  4:59802/149‑1 (MQ=255)
   gtgCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCgcgc                                                                                                                                         <  2:1884051/149‑1 (MQ=255)
          aTGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAg                                                                                                                                  <  2:316330/149‑1 (MQ=255)
              tGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCa                                                                                                                                   <  2:1789745/144‑1 (MQ=255)
              tGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCa                                                                                                                                   >  1:1789745/1‑144 (MQ=255)
              tGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGa                                                                                                                              >  3:539915/1‑149 (MQ=255)
              tGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgcca                                                                                                                                       >  2:810898/1‑138 (MQ=255)
              tGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgcca                                                                                                                                       <  1:810898/140‑3 (MQ=255)
               gAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAgg                                                                                                                                 <  2:1582209/145‑1 (MQ=255)
               gAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAgg                                                                                                                                 >  1:1582209/1‑145 (MQ=255)
                 aaTCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAgg                                                                                                                           <  3:400088/149‑1 (MQ=255)
                   tCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGc                                                                                                                         >  2:1464747/1‑149 (MQ=255)
                   tCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGc                                                                                                                         <  3:1700895/149‑1 (MQ=255)
                        gCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa                                                                                                                    <  1:1236268/149‑1 (MQ=255)
                         cGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAAc                                                                                                                   <  2:1169790/149‑1 (MQ=255)
                           gTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAgg                                                                                                                           <  3:1782804/139‑1 (MQ=255)
                             ggATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGc                                                                                                               <  1:1464747/149‑1 (MQ=255)
                             ggATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGc                                                                                                               >  3:87516/1‑149 (MQ=255)
                                    ccGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGa                                                                                                        >  3:714324/1‑149 (MQ=255)
                                     cGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAg                                                                                                          <  1:1693361/146‑1 (MQ=255)
                                         gACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGc                                                                                                   >  2:1882628/1‑149 (MQ=255)
                                         gACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGc                                                                                                   <  2:1671735/149‑1 (MQ=255)
                                            tAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTc                                                                                                <  3:1440023/149‑1 (MQ=255)
                                                tGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAg                                                                                            >  1:1185820/1‑149 (MQ=255)
                                                 ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgc                                                                                           <  4:714324/149‑1 (MQ=255)
                                                 ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgc                                                                                           >  1:125009/1‑149 (MQ=255)
                                                  gCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg                                                                                          >  2:916373/1‑149 (MQ=255)
                                                    aGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCTATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCa                                                                                        >  3:809571/1‑149 (MQ=255)
                                                    aGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCa                                                                                        >  1:653608/1‑149 (MQ=255)
                                                     ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGaa                                                                                                       >  1:515333/1‑133 (MQ=255)
                                                     ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGaa                                                                                                       <  2:515333/133‑1 (MQ=255)
                                                     ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAt                                                                                       >  3:1096089/1‑149 (MQ=255)
                                                       aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCt                                                                                               >  3:779858/1‑139 (MQ=255)
                                                       aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCt                                                                                               <  4:779858/139‑1 (MQ=255)
                                                       aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTa                                                                                     >  2:1745825/1‑149 (MQ=255)
                                                        aGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACa                                                                                                           >  4:1698253/1‑126 (MQ=255)
                                                        aGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACa                                                                                                           <  3:1698253/126‑1 (MQ=255)
                                                         gCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                   >  1:874032/1‑149 (MQ=255)
                                                           aCTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCt                                                                                 >  3:1739233/1‑149 (MQ=255)
                                                             tGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAt                                                                                       <  4:1573120/141‑1 (MQ=255)
                                                             tGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAt                                                                                       >  3:1573120/1‑141 (MQ=255)
                                                               gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACa                                                                                                     <  4:40924/125‑1 (MQ=255)
                                                               gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACa                                                                                                     >  3:40924/1‑125 (MQ=255)
                                                               gATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGtt                                                                             <  2:1185820/149‑1 (MQ=255)
                                                                   aCGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTc                                                                         <  1:916373/149‑1 (MQ=255)
                                                                         aaaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGt                                                                              <  4:1739462/138‑1 (MQ=255)
                                                                         aaaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGt                                                                              >  3:1739462/1‑138 (MQ=255)
                                                                          aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTa                                                                                     >  4:746523/1‑130 (MQ=255)
                                                                          aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTa                                                                                     <  3:746523/130‑1 (MQ=255)
                                                                            aaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGAt                                                                >  2:125469/1‑149 (MQ=255)
                                                                                 ccGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTg                                                           <  4:539915/149‑1 (MQ=255)
                                                                                   gggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc                                                         >  2:1753446/1‑149 (MQ=255)
                                                                                   gggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc                                                         <  1:1753446/149‑1 (MQ=255)
                                                                                         tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                        <  2:91414/144‑1 (MQ=255)
                                                                                         tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg                                                        >  1:91414/1‑144 (MQ=255)
                                                                                         tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAtt                                                   <  1:1745825/149‑1 (MQ=255)
                                                                                              ctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTaaa                                              <  2:653608/149‑1 (MQ=255)
                                                                                               tgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAg                                             >  2:364135/1‑149 (MQ=255)
                                                                                                         tataTCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGt                                                                                                                       >  4:1232575/1‑65 (MQ=255)
                                                                                                          atatCAATTTCACCGGGCTGGCGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTTAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGCCGCAATTTTAAAGCGGGCGAACAg                                  >  2:274651/1‑149 (MQ=255)
                                                                                                           tatCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGc                                 >  2:1210586/1‑149 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt                                                    <  3:648117/128‑1 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt                                                    >  4:648117/1‑128 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         <  3:1209233/139‑1 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         >  4:1209233/1‑139 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         <  1:1141856/139‑1 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         >  2:1141856/1‑139 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCaa                               >  2:311828/1‑149 (MQ=255)
                                                                                                             tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCaa                               >  2:671199/1‑149 (MQ=255)
                                                                                                              cAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCaaa                              <  2:874032/149‑1 (MQ=255)
                                                                                                               aaTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         >  2:1558980/1‑137 (MQ=255)
                                                                                                               aaTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCggg                                         <  1:1558980/137‑1 (MQ=255)
                                                                                                                 tttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTa                           >  1:494430/1‑149 (MQ=255)
                                                                                                                 tttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTa                           >  1:1450313/1‑149 (MQ=255)
                                                                                                                 tttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTa                           <  4:1096089/149‑1 (MQ=255)
                                                                                                                   tCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGttt                                                                            >  1:1173663/1‑98 (MQ=255)
                                                                                                                    cACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCa                                >  3:151226/1‑141 (MQ=255)
                                                                                                                     aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                   >  3:280604/1‑89 (MQ=255)
                                                                                                                     aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                                                                   <  4:280604/89‑1 (MQ=255)
                                                                                                                     aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatcat                       >  1:825400/1‑149 (MQ=255)
                                                                                                                       cGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCg                     >  4:1027912/1‑149 (MQ=255)
                                                                                                                              gtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTgg                                                          >  4:860931/1‑105 (MQ=255)
                                                                                                                                ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt                                                    <  4:1425091/109‑1 (MQ=255)
                                                                                                                                ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt                                                    >  3:1425091/1‑109 (MQ=255)
                                                                                                                                ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGc             >  4:1640278/1‑148 (MQ=255)
                                                                                                                                ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGc             <  3:1640278/148‑1 (MQ=255)
                                                                                                                                  aaaGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTg          <  2:825400/149‑1 (MQ=255)
                                                                                                                                   aaGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTgg         <  1:125469/149‑1 (MQ=255)
                                                                                                                                      ttgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatca                        >  2:1686791/3‑131 (MQ=255)
                                                                                                                                      ttgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTatca                        <  1:1686791/129‑1 (MQ=255)
                                                                                                                                       tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGc        <  2:346273/145‑1 (MQ=255)
                                                                                                                                       tgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGc        >  1:346273/2‑146 (MQ=255)
                                                                                                                                        gcgcGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGc                                        <  4:497460/113‑1 (MQ=255)
                                                                                                                                        gcgcGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGc                                        >  3:497460/1‑113 (MQ=255)
                                                                                                                                        gcgcGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGAt    <  4:1700484/149‑1 (MQ=255)
                                                                                                                                         cgcgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATg   >  2:1739505/1‑149 (MQ=255)
                                                                                                                                          gcgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAtt                                                   >  2:1258176/1‑100 (MQ=255)
                                                                                                                                          gcgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATgc  <  2:125009/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GTGGTGCCGCATGGTGAAATCGAGGCGGTGGATCAGCCGTGGACTAACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAGCGGGCGAACAGCAAATTATCATCGGCAAAGGCGTGGCGGATGC  >  NC_000913/1177503‑1177789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: