| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,187,090 | G→C | 16.0% | intergenic (+20/+29) | pepT → / ← roxA | peptidase T/50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,187,090 | 0 | G | C | 16.0% | 146.0 / 20.6 | 50 | intergenic (+20/+29) | pepT/roxA | peptidase T/50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase |
| Reads supporting (aligned to +/- strand): ref base G (22/20); new base C (3/5); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
CCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGT > NC_000913/1186942‑1187236 | ccGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCAtg < 2:14726/149‑1 (MQ=255) ccGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCTGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc > 3:1785408/1‑149 (MQ=255) ccGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc < 4:882776/149‑1 (MQ=255) ccTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCAt < 3:1549998/149‑1 (MQ=255) cTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCa < 1:674504/147‑1 (MQ=255) cTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCa > 2:674504/1‑147 (MQ=255) aCTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc > 4:19921/1‑137 (MQ=255) aCTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc < 3:19921/137‑1 (MQ=255) aCAACTATCATGGTAAGCATGCGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAAc < 4:1785408/149‑1 (MQ=255) aaCTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCaaa > 3:538871/1‑146 (MQ=255) aaCTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCaaa < 4:538871/146‑1 (MQ=255) aaCTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCAt < 1:279817/130‑1 (MQ=255) aaCTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCAt > 2:279817/1‑130 (MQ=255) tCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAAt < 1:1762035/136‑1 (MQ=255) tCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAAt > 2:1762035/1‑136 (MQ=255) tGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCg > 2:259114/1‑149 (MQ=255) tAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCa > 4:1783197/1‑118 (MQ=255) tAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCa < 3:1783197/118‑1 (MQ=255) cATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGcc < 1:814473/111‑1 (MQ=255) cATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGcc > 2:814473/1‑111 (MQ=255) aTGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATc > 3:1866760/1‑115 (MQ=255) aTGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATc < 4:1866760/115‑1 (MQ=255) tGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCaa > 1:1823559/1‑149 (MQ=255) gTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATAc > 2:659352/1‑149 (MQ=255) ttGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATAccc < 2:1823559/149‑1 (MQ=255) tctGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc > 3:897123/1‑96 (MQ=255) tctGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGc < 4:897123/96‑1 (MQ=255) gAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGAt < 4:36134/90‑1 (MQ=255) gAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGAt > 3:36134/1‑90 (MQ=255) tATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCaa > 3:1440586/1‑128 (MQ=255) tATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCaa < 4:1440586/128‑1 (MQ=255) ggTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg > 2:679889/1‑149 (MQ=255) gTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGa < 1:259114/149‑1 (MQ=255) gTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGAt > 4:1636660/1‑149 (MQ=255) gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCAtgt > 4:1518082/1‑53 (MQ=255) gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCAtgt < 3:1518082/53‑1 (MQ=255) ttGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAg > 4:132078/1‑149 (MQ=255) cGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCa > 2:1541794/1‑149 (MQ=255) cGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCa < 1:679889/149‑1 (MQ=255) cGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCa < 1:659352/149‑1 (MQ=255) ttAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGc > 2:44425/1‑149 (MQ=255) ggCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCaaaa < 1:44425/149‑1 (MQ=255) cAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATtct < 1:1541794/149‑1 (MQ=255) cGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTAtt < 3:693977/87‑1 (MQ=255) cGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTAtt > 4:693977/1‑87 (MQ=255) gAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCa < 3:132078/149‑1 (MQ=255) gAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCtt < 3:797767/114‑1 (MQ=255) gAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCtt > 4:797767/1‑114 (MQ=255) aTGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCt > 4:1070742/1‑149 (MQ=255) aTGCGGCATCACATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgc < 1:1110306/89‑1 (MQ=39) ggCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg > 3:188422/1‑147 (MQ=255) ggCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg < 4:188422/147‑1 (MQ=255) cATGTGATGCCGCATCCGGCTTAAATCCAAAC‑TACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGt > 2:688450/1‑149 (MQ=255) | CCGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGT > NC_000913/1186942‑1187236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |