Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,256,416 A→T 100% intergenic (‑464/+305) ycgV ← / ← ychF putative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,256,4160AT98.7% 576.1 / ‑1.7 152intergenic (‑464/+305)ycgV/ychFputative adhesin/catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (79/71);  total (80/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 152 total aligned reads displayed.

CTAATTAGTTATGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTT  >  NC_000913/1256271‑1256558
                                                                                                                                                 |                                                                                                                                              
cTAATTAGTTATGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTg                                                                                                                                             <  3:1316186/149‑1 (MQ=255)
      aGTTATGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATc                                                                                                                                       <  3:757277/149‑1 (MQ=255)
         tATGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCAtt                                                                                                                                    <  3:1086739/149‑1 (MQ=255)
           tGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCAtttt                                                                                                                                  >  2:714588/1‑149 (MQ=255)
           tGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCAtttt                                                                                                                                  >  2:294620/1‑149 (MQ=255)
                         gATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCat                                                                                                                    >  4:902985/1‑149 (MQ=255)
                              aTCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTgg                                                                                                               >  2:545685/1‑149 (MQ=255)
                                 tGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGc                                                                                                            <  1:524749/149‑1 (MQ=255)
                                  gCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGGTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCg                                                                                                           >  3:743380/1‑149 (MQ=255)
                                          aaaaCAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAt                                                                                                   >  1:1435959/1‑149 (MQ=255)
                                           aaaCAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAtt                                                                                                  <  4:462657/149‑1 (MQ=255)
                                           aaaCAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAtt                                                                                                  <  1:294620/149‑1 (MQ=255)
                                            aaCAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCt                                                                                                                             <  2:693528/121‑1 (MQ=255)
                                            aaCAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCt                                                                                                                             >  1:693528/1‑121 (MQ=255)
                                                     cgcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCt                                                                                        <  2:1435959/149‑1 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCGTTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCtt                                                                                                                            >  3:1290210/1‑112 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTc                                                                                                                                 >  2:858771/1‑107 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCtt                                                                                                                            <  4:1290210/112‑1 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTg                                                                                                         <  3:552265/131‑1 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCtg                                                                                       >  4:770619/1‑149 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCtg                                                                                       >  4:1627276/1‑149 (MQ=255)
                                                      gcCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCAt                                                                                                                               <  2:633426/109‑1 (MQ=255)
                                                       ccATTTTTGCATTACTAATAGGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAt                                                                                                   >  2:1831645/1‑136 (MQ=255)
                                                       ccATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAt                                                                                                   <  1:1831645/136‑1 (MQ=255)
                                                       ccATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCtgt                                                                                      >  3:1159157/1‑149 (MQ=255)
                                                          tttttGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGaa                                                                                   >  3:1549934/1‑149 (MQ=255)
                                                             ttGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAggg                                                                                <  4:1549934/149‑1 (MQ=255)
                                                               gCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAggggg                                                                              <  3:902985/149‑1 (MQ=255)
                                                                   tACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTg                                                                                                                <  4:1737401/111‑1 (MQ=255)
                                                                   tACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGAt                                                                                             >  2:697656/1‑130 (MQ=255)
                                                                      taataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCAt                                                                                                                               >  2:1141488/1‑93 (MQ=255)
                                                                      taataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGAtgt                                                                       >  3:49323/1‑149 (MQ=255)
                                                                      taataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTCTGCTTCTGGTTATCATTTTCAt                                                                                                                               <  1:1141488/93‑1 (MQ=255)
                                                                       aataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGAtgtg                                                                      >  2:1148123/1‑149 (MQ=255)
                                                                        ataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGcc                                                                                                                         >  3:72309/1‑97 (MQ=255)
                                                                        ataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGcc                                                                                                                         <  4:72309/97‑1 (MQ=255)
                                                                        ataaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATgtgt                                                                     >  2:1081442/1‑149 (MQ=255)
                                                                         taaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTa                                                                    <  1:714588/149‑1 (MQ=255)
                                                                         taaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTa                                                                    <  1:1148123/149‑1 (MQ=255)
                                                                          aaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAg                                                                   <  1:545685/149‑1 (MQ=255)
                                                                          aaGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAg                                                                   <  3:770619/149‑1 (MQ=255)
                                                                            gAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGa                                                                                                              <  4:64017/104‑1 (MQ=255)
                                                                                  gCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTa                                                                    <  1:1486767/140‑1 (MQ=255)
                                                                                  gCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTa                                                                    >  2:1486767/1‑140 (MQ=255)
                                                                                    aaaaaaCATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGAtgt                                                                       >  1:623635/1‑135 (MQ=255)
                                                                                     aaaaaCATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATc                                                                                            >  4:1354219/1‑113 (MQ=255)
                                                                                     aaaaaCATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATc                                                                                            <  3:1354219/113‑1 (MQ=255)
                                                                                      aaaaCATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAAc                                                       <  3:862047/149‑1 (MQ=255)
                                                                                          cATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTAc                                                   <  1:1418637/149‑1 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCAtt                                                                                                                                    <  3:472650/67‑1 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCAtt                                                                                                                                    >  4:472650/1‑67 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGc                                                                                                            >  2:1225235/1‑91 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGc                                                                                                            >  4:1403099/1‑91 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGc                                                                                                            <  3:1403099/91‑1 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGc                                                                                                            <  1:1225235/91‑1 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGAt                                                                                             >  4:533353/1‑106 (MQ=255)
                                                                                           aTCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGAt                                                                                             <  3:533353/106‑1 (MQ=255)
                                                                                               atatTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTtat                                              >  4:1847646/1‑149 (MQ=255)
                                                                                               atatTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTtat                                              >  2:1466166/1‑149 (MQ=255)
                                                                                                  ttCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGGTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTATGATCAGCTGTGTAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATc                                           >  3:375353/1‑149 (MQ=255)
                                                                                                  ttCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATa                                                              <  1:1059025/130‑1 (MQ=255)
                                                                                                  ttCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATa                                                              >  2:1059025/1‑130 (MQ=255)
                                                                                                  ttCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACt                                                      >  2:458658/1‑138 (MQ=255)
                                                                                                  ttCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACt                                                      <  1:458658/138‑1 (MQ=255)
                                                                                                     aTTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGtt                                                <  1:695817/141‑1 (MQ=255)
                                                                                                       ttttGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGc                                      <  3:1847646/149‑1 (MQ=255)
                                                                                                       ttttGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGc                                      <  1:1466166/149‑1 (MQ=255)
                                                                                                             tCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCCTATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTAc                                                   <  2:1179363/130‑1 (MQ=255)
                                                                                                             tCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTAc                                                   >  3:1820937/1‑130 (MQ=255)
                                                                                                             tCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTAc                                                   >  1:1179363/1‑130 (MQ=255)
                                                                                                               aaTGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAttt                                                                                                 <  3:1666388/82‑1 (MQ=255)
                                                                                                               aaTGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAttt                                                                                                 >  4:1666388/1‑82 (MQ=255)
                                                                                                               aaTGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATgaga                              >  4:299874/1‑149 (MQ=255)
                                                                                                                 tGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATg                                 >  1:985070/1‑144 (MQ=255)
                                                                                                                 tGGGTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATg                                 <  2:985070/144‑1 (MQ=255)
                                                                                                                  gggTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGatat                           >  2:1870142/1‑149 (MQ=255)
                                                                                                                  gggTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGatag                           >  4:1216907/1‑148 (MQ=255)
                                                                                                                   ggTTTTCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATc                          <  1:81770/149‑1 (MQ=255)
                                                                                                                     ttttCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATgag                               <  4:785274/142‑1 (MQ=255)
                                                                                                                       ttCTGGCCTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAACAACTTACGTTATATCACTGCTAATGAGATATCCGGc                      <  4:1159157/149‑1 (MQ=255)
                                                                                                                             ccTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGa                <  1:1870142/149‑1 (MQ=255)
                                                                                                                              cTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAg                                                                   >  2:1684584/1‑97 (MQ=255)
                                                                                                                              cTATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGaa               <  4:375353/149‑1 (MQ=255)
                                                                                                                               tATCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAac              <  4:502446/149‑1 (MQ=255)
                                                                                                                                aTCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTa                                                                    >  3:293464/1‑94 (MQ=255)
                                                                                                                                aTCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAaca             >  4:1147774/1‑149 (MQ=255)
                                                                                                                                 tCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGAt                                                                                                       <  2:379774/58‑1 (MQ=255)
                                                                                                                                 tCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGAtgt                                                                       <  4:988469/90‑1 (MQ=255)
                                                                                                                                 tCGTTGTTATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGAtgt                                                                       >  3:988469/1‑90 (MQ=255)
                                                                                                                                   gttgttATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAAt                                  <  2:527168/125‑1 (MQ=255)
                                                                                                                                   gttgttATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACtt          >  3:1484590/1‑149 (MQ=255)
                                                                                                                                    ttgttATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTa         >  4:91166/1‑149 (MQ=255)
                                                                                                                                      gttATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGaa               >  4:269058/1‑141 (MQ=255)
                                                                                                                                       ttATTGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGt      <  3:990761/149‑1 (MQ=255)
                                                                                                                                          ttGTGCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAAttt                                                                                                 >  3:1631655/1‑55 (MQ=255)
                                                                                                                                           tgtgCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCttt  <  1:1081442/149‑1 (MQ=255)
                                                                                                                                            gtgCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATg       <  2:208774/143‑1 (MQ=255)
                                                                                                                                            gtgCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATg       >  1:208774/1‑143 (MQ=255)
                                                                                                                                             tgCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGat                             <  4:1078051/120‑1 (MQ=255)
                                                                                                                                             tgCTTCTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGat                             >  3:1078051/1‑120 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTAATTAGTTATGGGAATTTCGCTTGATGTATCTGCATAACAAAAACAATATGCGCCATTTTTGCATTACTAATAAGAAAAAGCAAAAAACATCGATATTCATTTTTGGTCAATGGGTTTTCTGGCCTATCGTTGTTATTGTGCTACTGGTTATCATTTTCATCTTGCCAGCATATTGGAGCGTGATCAATTTTGATCAGCTGTGAAGGGGGCTGATGTGTAGTGATACAAAAACTTACGTTATATCACTGCTAATGAGATATCCGGCATCTGAACACTTATGTCTTT  >  NC_000913/1256271‑1256558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: