| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,664,591 | A→C | 11.5% | E29A (GAA→GCA) ‡ | dmsD → | twin‑argninine leader‑binding protein for DmsA and TorA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,664,591 | 0 | A | C | 11.5% | 191.6 / 16.8 | 61 | E29A (GAA→GCA) ‡ | dmsD | twin‑argninine leader‑binding protein for DmsA and TorA |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base C (4/3); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
AATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTGTTTGTC > NC_000913/1664445‑1664736 | aaTGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAg < 1:1116957/149‑1 (MQ=255) gTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAG‑GTGCGGCTTCCGCActctct > 1:1524136/1‑140 (MQ=255) ttGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCgg < 2:1643171/144‑1 (MQ=255) ttGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCgg > 1:1643171/1‑144 (MQ=255) cGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGc > 1:685642/1‑136 (MQ=255) cGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGc < 2:685642/136‑1 (MQ=255) ccGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAG‑GTGCGGCTTCCGCACTCTCTgga < 3:184090/124‑2 (MQ=255) ccGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAG‑GTGCGGCTTCCGCACTCTCTgga > 4:184090/1‑123 (MQ=255) cacaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAG‑GTGCGGCTTCCGCACTCTCTgga > 4:352476/1‑120 (MQ=255) cacaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAG‑GTGCGGCTTCCGCACTCTCTgga < 3:352476/121‑2 (MQ=255) aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTAc > 4:548376/1‑149 (MQ=255) gAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa > 4:20780/1‑149 (MQ=255) cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt < 1:152908/132‑1 (MQ=255) cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt > 2:152908/1‑132 (MQ=255) ccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGt > 3:1554352/1‑149 (MQ=255) ttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAtt < 3:548376/149‑1 (MQ=255) ttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg < 4:1554352/149‑1 (MQ=255) aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt < 1:1089503/137‑1 (MQ=255) aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt > 2:1089503/1‑137 (MQ=255) gATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGt < 3:20780/149‑1 (MQ=255) cTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCtttt > 3:1507179/1‑149 (MQ=255) tgtTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc < 2:1387855/106‑1 (MQ=255) tgtTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGc > 1:1387855/1‑106 (MQ=255) ttGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGAc < 4:19363/72‑1 (MQ=255) ttGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGAc > 3:19363/1‑72 (MQ=255) gggCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg < 1:1540282/85‑1 (MQ=255) gggCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg > 2:1540282/1‑85 (MQ=255) gcgcgcTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCa < 3:175138/63‑1 (MQ=255) gcgcgcTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCa > 4:175138/1‑63 (MQ=255) gcgcTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCa > 3:1894094/1‑149 (MQ=255) gcTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACg > 1:1549931/1‑149 (MQ=255) gcTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACg > 1:76170/1‑149 (MQ=255) gTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGccc > 2:665110/1‑149 (MQ=255) gTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGccc > 4:564414/1‑149 (MQ=255) gTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGccc > 4:252659/1‑149 (MQ=255) ttaCGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTg < 1:1617916/72‑1 (MQ=255) ttaCGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTg > 2:1617916/1‑72 (MQ=255) taCGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTg < 4:1507179/149‑1 (MQ=255) aaccAGAG‑GTGCGGCTTCCGCACTCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGa < 2:955469/122‑1 (MQ=38) aaccAGAG‑GTGCGGCTTCCGCACTCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGa > 1:955469/3‑124 (MQ=38) tCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGc > 1:1517468/1‑149 (MQ=255) tCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGc > 1:1018841/1‑149 (MQ=255) tCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGc < 1:665110/149‑1 (MQ=255) tCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGc < 3:564414/149‑1 (MQ=255) cAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGa < 3:942896/99‑1 (MQ=255) cAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGa > 4:942896/1‑99 (MQ=255) agagagTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGa > 2:530828/1‑110 (MQ=255) agagagTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGa < 1:530828/110‑1 (MQ=255) gagTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgt < 3:252659/149‑1 (MQ=255) agTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgtt > 3:1487747/1‑149 (MQ=255) agTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgtt < 4:1894094/149‑1 (MQ=255) agTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgtt < 2:1549931/149‑1 (MQ=255) gTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgttt < 2:1517468/149‑1 (MQ=255) tGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGtttgtttg > 1:194566/1‑149 (MQ=255) gCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTttgtttgt < 2:76170/149‑1 (MQ=255) cGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTGTTTGTc > 3:1579536/1‑149 (MQ=255) cGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTGTTTGTc < 2:194566/149‑1 (MQ=255) ggAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCtttt > 2:812168/1‑97 (MQ=255) ggAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCtgtt < 1:812168/97‑1 (MQ=255) gAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgt < 4:1062035/142‑1 (MQ=255) gAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCgtttgt > 3:1062035/1‑142 (MQ=255) | AATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCAC‑CTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCCGCTGGTGACGGCTTTTCAGACACAGTGTGAAGAGACTCACGCCCAGGCCTGGCAGCGTTTGTTTGTC > NC_000913/1664445‑1664736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |