Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,707,755 T→A 5.1% A279A (GCT→GCA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,7550TA 5.1% 414.8 / 7.9 114A279A (GCT→GCArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (62/46);  new base A (4/2);  total (66/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 115 total aligned reads displayed.

CTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGATTCTGC  >  NC_000913/1707608‑1707902
                                                                                                                                                   |                                                                                                                                                   
ctctAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCtt                                                                                                                                                    <  2:54062/149‑1 (MQ=255)
 tctAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTa                                                                                                                                                   >  2:735349/1‑149 (MQ=255)
                   cGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGt                                                                                                                                 >  1:132587/1‑149 (MQ=255)
                      gTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCCAAACGTCGGCACTGCTTAt                                                                                                                                                  <  3:1118514/129‑1 (MQ=255)
                      gTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTAt                                                                                                                                                  >  4:1118514/1‑129 (MQ=255)
                      gTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              <  3:669990/149‑1 (MQ=255)
                      gTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              <  3:1172211/149‑1 (MQ=255)
                            ccaaccaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAg                                                                                                                                              <  4:914805/127‑1 (MQ=255)
                            ccaaccaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAg                                                                                                                                              >  3:914805/1‑127 (MQ=255)
                             caaccaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCAACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              <  1:1664244/142‑1 (MQ=255)
                             caaccaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCAACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              >  2:1664244/1‑142 (MQ=255)
                               accaaATATCCTTCTGGCGGTGCTAAACAATTAACCTAGATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGa                                                                                                                     >  4:1564532/1‑149 (MQ=255)
                               accaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTa                                                                                                                                                   >  3:1864839/1‑119 (MQ=255)
                               accaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGa                                                                                                                     >  1:200240/1‑149 (MQ=255)
                               accaaATATCCTTCTGGCGGTGCTAAACAATCAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTa                                                                                                                                                   <  4:1864839/119‑1 (MQ=255)
                                ccaaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAg                                                                                                                    >  3:1786630/1‑149 (MQ=255)
                                 caaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGc                                                                                                                   <  2:200240/149‑1 (MQ=255)
                                 caaATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGc                                                                                                                   <  4:1786630/149‑1 (MQ=255)
                                         cTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTAcc                                                                                                           >  3:1480552/1‑149 (MQ=255)
                                          ttCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGAtt                                                                                                              <  4:472635/145‑1 (MQ=255)
                                          ttCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGAtt                                                                                                              >  3:472635/1‑145 (MQ=255)
                                          ttCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCg                                                                                                          >  4:850203/1‑149 (MQ=255)
                                             tGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGt                                                                                                                                             <  2:1208805/111‑1 (MQ=255)
                                             tGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGt                                                                                                                                             >  1:1208805/1‑111 (MQ=255)
                                               gCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATg                                                                                                                         <  4:1426333/129‑1 (MQ=255)
                                               gCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATg                                                                                                                         >  3:1426333/1‑129 (MQ=255)
                                                    gCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGtgttgt                                                                                                <  1:998489/149‑1 (MQ=255)
                                                     cTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTa                                                                                               <  3:1597894/149‑1 (MQ=255)
                                                     cTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTa                                                                                               >  3:263195/1‑149 (MQ=255)
                                                      tAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTaa                                                                                              <  3:850203/149‑1 (MQ=255)
                                                      tAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTaa                                                                                              >  1:159431/1‑149 (MQ=255)
                                                      tAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTaa                                                                                              >  1:663688/1‑149 (MQ=255)
                                                        aaCAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGa                                                                                                         <  1:909145/136‑1 (MQ=255)
                                                        aaCAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGa                                                                                                         >  2:909145/1‑136 (MQ=255)
                                                             ttAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGAc                                                                                       <  4:1402344/149‑1 (MQ=255)
                                                                aCCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTgg                                                                                    >  3:117895/1‑149 (MQ=255)
                                                                aCCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTgg                                                                                    >  3:121199/1‑149 (MQ=255)
                                                                  cTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCg                                                                                  >  3:1764150/1‑149 (MQ=255)
                                                                  cTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCg                                                                                  >  1:84552/1‑149 (MQ=255)
                                                                   tACATTCTGGCCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCgt                                                                                 >  4:1629890/1‑148 (MQ=255)
                                                                   tACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGCGCCGTTATTGATGACGTGCCGATTACCGAGCGTGTTGTAAGCCTGACTGGCgg                                                                                 >  4:296496/1‑148 (MQ=255)
                                                                    aCATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGaa                                                                                <  4:587985/149‑1 (MQ=255)
                                                                    aCATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGaa                                                                                <  4:263195/149‑1 (MQ=255)
                                                                    aCATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGaa                                                                                <  3:1564532/149‑1 (MQ=255)
                                                                           gACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcg                                                                         >  1:949863/1‑149 (MQ=255)
                                                                           gACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcg                                                                         >  3:647061/1‑149 (MQ=255)
                                                                           gACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcg                                                                         >  1:813547/1‑149 (MQ=255)
                                                                             ccGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcgct                                                                       >  2:1672819/1‑149 (MQ=255)
                                                                             ccGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcgct                                                                       <  2:663688/149‑1 (MQ=255)
                                                                                ggAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATAAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAAtcgctcgc                                                                    <  3:1629890/149‑1 (MQ=255)
                                                                                 gAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCg                                                                                  <  3:1563393/134‑1 (MQ=255)
                                                                                 gAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCg                                                                                  >  4:1563393/1‑134 (MQ=255)
                                                                                   aGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCg                                                                 >  4:1130629/1‑149 (MQ=255)
                                                                                    gCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAGCGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCgg                                                                >  4:1235448/1‑149 (MQ=255)
                                                                                    gCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              <  4:867764/87‑1 (MQ=255)
                                                                                    gCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTAt                                                                                                                              >  3:867764/1‑87 (MQ=255)
                                                                                        gTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCaa                                                            >  4:1599359/1‑149 (MQ=255)
                                                                                        gTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCaa                                                            >  3:715766/1‑149 (MQ=255)
                                                                                         ttCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAAc                                                           >  2:31696/1‑149 (MQ=255)
                                                                                         ttCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAAc                                                           >  4:1540132/1‑149 (MQ=255)
                                                                                          tCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACt                                                                                      <  3:1549697/121‑1 (MQ=255)
                                                                                          tCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGCTGTAACCCTGACt                                                                                      >  4:1549697/1‑121 (MQ=255)
                                                                                             acaTGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCt                                                       <  1:1672819/149‑1 (MQ=255)
                                                                                               aTGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTgg                                                     <  2:813547/149‑1 (MQ=255)
                                                                                               aTGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTgg                                                     <  2:949863/149‑1 (MQ=255)
                                                                                                    gggcgTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACg                                                >  1:692287/1‑149 (MQ=255)
                                                                                                         ttCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTgg                                           <  3:1540132/149‑1 (MQ=255)
                                                                                                         ttCATCCGATATCGGCGTATTAATGAAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTgg                                           <  3:1235448/149‑1 (MQ=255)
                                                                                                             tCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCt                                                       <  1:345328/133‑1 (MQ=255)
                                                                                                             tCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCt                                                       >  2:345328/1‑133 (MQ=255)
                                                                                                             tCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGAc                                       <  2:84552/149‑1 (MQ=255)
                                                                                                                gATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTggg                                          <  4:321147/143‑1 (MQ=255)
                                                                                                                gATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTggg                                          >  3:321147/1‑143 (MQ=255)
                                                                                                                       gCGTATTAATGCAAAACGTCGGCACTGGTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGaa                                                                                >  4:1741024/1‑98 (MQ=255)
                                                                                                                       gCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGCCTGGCGaa                                                                                <  3:1741024/98‑1 (MQ=255)
                                                                                                                            ttAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCAttt                        >  3:328793/1‑149 (MQ=255)
                                                                                                                                 gCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAAc                                                           >  4:950283/1‑109 (MQ=255)
                                                                                                                                   aaaaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCAtt                         <  4:400485/141‑1 (MQ=255)
                                                                                                                                   aaaaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCAtt                         >  3:400485/1‑141 (MQ=255)
                                                                                                                                   aaaaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAat                 <  4:121199/149‑1 (MQ=255)
                                                                                                                                    aaaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAatg                >  2:1718064/1‑149 (MQ=255)
                                                                                                                                     aaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTggg                                          <  3:261638/122‑1 (MQ=255)
                                                                                                                                     aaCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAatga               >  1:1648143/1‑149 (MQ=255)
                                                                                                                                      aCGTCGGCACTGCTTTTGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAatgat              >  4:1023118/1‑149 (MQ=255)
                                                                                                                                      aCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGTGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAatgat              <  4:1764150/149‑1 (MQ=255)
                                                                                                                                      aCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCa                                   >  3:1055024/1‑128 (MQ=255)
                                                                                                                                      aCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCa                                   <  4:1055024/128‑1 (MQ=255)
                                                                                                                                      aCGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAatgat              >  1:1840057/1‑149 (MQ=255)
                                                                                                                                         tCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGcc           <  1:31696/149‑1 (MQ=255)
                                                                                                                                          tttcACTGCATAAGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCg          >  3:420060/4‑149 (MQ=255)
                                                                                                                                            ttaCTGCATATGCAATGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCgg                                                                >  4:343695/3‑93 (MQ=255)
                                                                                                                                            tcACTGCATAAGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCgg                                                                <  3:343695/92‑1 (MQ=255)
                                                                                                                                            gCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTg                                                      <  3:957186/103‑1 (MQ=255)
                                                                                                                                              aCTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGAtt      >  4:911457/1‑149 (MQ=255)
                                                                                                                                              aCTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGAtt      >  2:1714942/1‑149 (MQ=255)
                                                                                                                                              aCTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGAtt      >  1:516815/1‑149 (MQ=255)
                                                                                                                                                tGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGt                                 <  4:887680/120‑1 (MQ=255)
                                                                                                                                                tGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGt                                 >  3:887680/1‑120 (MQ=255)
                                                                                                                                                tGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGATTCt    >  1:1723976/1‑149 (MQ=255)
                                                                                                                                                  cTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGATTCTGc  >  4:234386/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTCTAACGATATTTCTCTGCGGGTGATTCCAACCAAATATCCTTCTGGCGGTGCTAAACAATTAACCTACATTCTGACCGGGAAGCAGGTTCCACATGGCGGGCGTTCATCCGATATCGGCGTATTAATGCAAAACGTCGGCACTGCTTATGCAGTGAAACGTGCCGTTATTGATGGCGAGCCGATTACCGAGCGTGTTGTAACCCTGACTGGCGAAGCAATCGCTCGCCCGGGCAACGTCTGGGCACGGCTGGGGACGCCAGTGCGTCATTTATTGAATGATGCCGGATTCTGC  >  NC_000913/1707608‑1707902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: