Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,764,701	G→A	13.5%	intergenic (‑315/+12)	sufA ← / ← rydB	Fe‑S cluster assembly protein/novel sRNA, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,701	0	G	A	13.5%	159.4 / 11.1	58	intergenic (‑315/+12)	sufA/rydB	Fe‑S cluster assembly protein/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base A (5/3);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGG  >  NC_000913/1764556‑1764845                                                                                                                                                   |                                                                                                                                                 ctcATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCtt                                                                                                                                               <  2:830959/149‑1 (MQ=255)   cATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTG‑TCGCACAGACTTaa                                                                                                                                             <  3:987054/149‑1 (MQ=255)       gTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             >  2:680445/1‑145 (MQ=255)       gTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             <  1:680445/145‑1 (MQ=255)               aTGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            >  4:148945/1‑138 (MQ=255)               aTGGTTATTATCTAAGGAGCATTAAGGCTGTAATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            <  3:148945/138‑1 (MQ=255)                    tattatCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             >  3:1467143/1‑132 (MQ=255)                    tattatCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             <  4:1467143/132‑1 (MQ=255)                                        ggCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActc                                                                                                        >  2:1173659/1‑149 (MQ=255)                                                  aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            >  2:5195/1‑103 (MQ=255)                                                  aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            <  1:5195/103‑1 (MQ=255)                                                          aaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            >  4:1323291/1‑95 (MQ=255)                                                          aaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAg                                                                                                                                            <  3:1323291/95‑1 (MQ=255)                                                               tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCgg                                                                                                                          <  3:371978/108‑1 (MQ=255)                                                               tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCgg                                                                                                                          >  4:371978/1‑108 (MQ=255)                                                                   ttGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                                                                                      >  3:1386816/1‑124 (MQ=255)                                                                   ttGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                                                                                      <  4:1386816/124‑1 (MQ=255)                                                                                tttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAct                                                                                                         >  4:203006/1‑108 (MQ=255)                                                                                tttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAct                                                                                                         <  3:203006/108‑1 (MQ=255)                                                                                        aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                                                                           >  1:769031/1‑130 (MQ=255)                                                                                        aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                                                                           <  2:769031/130‑1 (MQ=255)                                                                                            gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             <  1:15816/60‑1 (MQ=255)                                                                                            gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTaa                                                                                                                                             >  2:15816/1‑60 (MQ=255)                                                                                             tAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                                   >  1:392336/1‑149 (MQ=255)                                                                                             tAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                                   >  1:144273/1‑149 (MQ=255)                                                                                               aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgc                                                 >  4:346495/1‑149 (MQ=255)                                                                                                   aGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGtt                                             >  4:1121893/1‑149 (MQ=255)                                                                                                       cAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTaa                                         <  2:392336/149‑1 (MQ=255)                                                                                                         gCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAaca                                       <  3:346495/149‑1 (MQ=255)                                                                                                             tCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACaaa                                   <  3:1121893/149‑1 (MQ=255)                                                                                                                 aaaTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc                                                                               <  2:61841/101‑1 (MQ=255)                                                                                                                 aaaTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGc                                                                               >  1:61841/1‑101 (MQ=255)                                                                                                                   aTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActc                                                                                                        <  2:1523293/74‑1 (MQ=255)                                                                                                                   aTAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActc                                                                                                        >  1:1523293/1‑74 (MQ=255)                                                                                                                          aaCCCTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAg                                                                                                    <  2:1136552/71‑1 (MQ=18)                                                                                                                          aaCCCTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAg                                                                                                    >  1:1136552/1‑71 (MQ=18)                                                                                                                           aCCCTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgggg                                                                                >  4:680580/1‑90 (MQ=37)                                                                                                                           aCCCTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgggg                                                                                <  3:680580/90‑1 (MQ=37)                                                                                                                            cccTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                                                                                 <  2:1450353/88‑1 (MQ=255)                                                                                                                            cccTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                                                                                 >  1:1450353/1‑88 (MQ=255)                                                                                                                             ccTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAGGAATAATCTCATAAAGCTAACCCGCCGTTTTaa                                         >  3:873017/1‑127 (MQ=255)                                                                                                                             ccTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAGGAATAATCTCATAAAGCTAACCCGCCGTTTTaa                                         <  4:873017/127‑1 (MQ=255)                                                                                                                             ccTTAAGCCTGTGTCG‑CACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtattat                   >  1:1426994/1‑149 (MQ=255)                                                                                                                              cTTAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTattatt                  >  2:1270934/1‑149 (MQ=255)                                                                                                                               ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGAt                                                                            <  2:1432908/90‑1 (MQ=255)                                                                                                                               ttAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGAt                                                                            >  1:1432908/1‑90 (MQ=255)                                                                                                                               ttAAGCCTGTG‑TCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttt                 >  3:1881758/1‑149 (MQ=255)                                                                                                                                    tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGa                             >  3:1230309/1‑132 (MQ=255)                                                                                                                                    tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGa                             <  4:1230309/132‑1 (MQ=255)                                                                                                                                    tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtat                      <  2:930174/139‑1 (MQ=255)                                                                                                                                    tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtat                      >  1:930174/1‑139 (MQ=255)                                                                                                                                    tCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa            >  2:1271485/1‑149 (MQ=255)                                                                                                                                            gACACAGGCTTAAGGGTTTCTACCCAATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc     <  2:144273/149‑1 (MQ=255)                                                                                                                                              cacaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAg   >  2:1441917/1‑149 (MQ=255)                                                                                                                                               acaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa            >  2:707842/1‑139 (MQ=255)                                                                                                                                               acaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa            <  1:707842/139‑1 (MQ=255)                                                                                                                                               acaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAgg  >  3:300596/1‑149 (MQ=255)                                                                                                                                               acaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAgg  >  1:1654170/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CTCATTGTTAGATAATGGTTATTATCTAAGGAGCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTG‑CGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGG  >  NC_000913/1764556‑1764845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑