Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,914,846 A→T 8.0% L230Q (CTG→CAG)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,914,8460AT 8.0% 517.4 / 28.5 151L230Q (CTG→CAG) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base A (74/64);  new base T (6/6);  total (80/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

Only 100 of 152 total aligned reads displayed.

TCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGA  >  NC_000913/1914702‑1914994
                                                                                                                                                |                                                                                                                                                    
tctcTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGgtg                                                                                                                                                  >  4:1473718/1‑149 (MQ=255)
 ctctTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGgtgt                                                                                                                                                 <  2:1641327/149‑1 (MQ=255)
    ttCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCt                                                                                                                                              >  4:1029659/1‑149 (MQ=255)
       cTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTgcg                                                                                                                                           >  3:136332/1‑149 (MQ=255)
           aaTACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGca                                                                                                                                       >  2:386861/1‑149 (MQ=255)
                   aaTTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCaaa                                                                                                                               <  3:407338/149‑1 (MQ=255)
                     tttGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGa                                                                                                                             <  3:1029659/149‑1 (MQ=255)
                         aTCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATa                                                                                                                         >  2:1630202/1‑149 (MQ=255)
                                    tGATAGCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTACAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCGGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATCCAg                                                                                                              <  1:951906/149‑1 (MQ=255)
                                     gATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGc                                                                                                             <  4:226727/149‑1 (MQ=255)
                                         tcttctACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATa                                                                                                                         <  1:854952/133‑1 (MQ=255)
                                         tcttctACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATa                                                                                                                         >  2:854952/1‑133 (MQ=255)
                                           ttctACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTggacg                                                                                                       >  1:1526382/1‑149 (MQ=255)
                                                  gCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTggacgcggacgc                                                                                                >  2:1244014/1‑149 (MQ=255)
                                                    gAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCg                                                                                              >  2:1160252/1‑149 (MQ=255)
                                                       ggTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCt                                                                                           <  1:1244014/149‑1 (MQ=255)
                                                        gTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCtt                                                                                          <  2:1526382/149‑1 (MQ=255)
                                                        gTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCtt                                                                                          <  2:1390821/149‑1 (MQ=255)
                                                          cTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTg                                                                                        <  4:1021863/149‑1 (MQ=255)
                                                             gCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGa                                                                                                                             <  4:114005/109‑1 (MQ=255)
                                                              ccTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCt                                                                                           <  1:458894/142‑1 (MQ=255)
                                                               cTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAt                                                                                   >  3:1107687/1‑149 (MQ=255)
                                                               cTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAt                                                                                   >  4:68610/1‑149 (MQ=255)
                                                               cTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAt                                                                                   >  1:1049393/1‑149 (MQ=255)
                                                                 gCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAttt                                                                                 <  4:69673/149‑1 (MQ=255)
                                                                  cTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAAt                                                                                                                                   <  2:1262486/98‑1 (MQ=255)
                                                                  cTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAAt                                                                                                                                   >  1:1262486/1‑98 (MQ=255)
                                                                   tATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCTGGTGTTCTga                                                                                                                                            >  3:1188685/1‑87 (MQ=255)
                                                                   tATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCTGGTGTTCTga                                                                                                                                            <  4:1188685/88‑2 (MQ=255)
                                                                   tATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGaa                                                                                                                   <  3:1794557/113‑1 (MQ=255)
                                                                   tATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTc                                                                                            <  1:362201/136‑1 (MQ=255)
                                                                    aTTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGaa                                                                                                                   >  1:1015531/1‑112 (MQ=255)
                                                                     ttGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTaa                                                                             >  1:972986/1‑149 (MQ=255)
                                                                        cAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTaa                                                                             <  3:1432438/146‑1 (MQ=255)
                                                                          aGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGa                                                                                                                             >  3:1625485/1‑96 (MQ=255)
                                                                          aGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTgg                                                                                                          <  3:29335/115‑1 (MQ=255)
                                                                          aGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTgg                                                                                                          >  4:29335/1‑115 (MQ=255)
                                                                          aGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACgg                                                                        >  3:1652053/1‑149 (MQ=255)
                                                                           gCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                                       <  1:1160252/149‑1 (MQ=255)
                                                                           gCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                                       <  2:542966/149‑1 (MQ=255)
                                                                                       aCGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTTTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccatc                                                             >  1:357055/1‑147 (MQ=255)
                                                                                       aCGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccatc                                                             <  2:357055/147‑1 (MQ=255)
                                                                                               aGCCTAAAAAACATGTTCCTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACATTCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGAc                                                   <  4:898610/149‑1 (MQ=255)
                                                                                               aGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGAc                                                   <  4:125200/149‑1 (MQ=255)
                                                                                                gCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGCCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGAcc                                                  >  2:1881582/1‑149 (MQ=255)
                                                                                                 ccTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCaaa                                                                                                                               <  1:1059720/71‑1 (MQ=255)
                                                                                                 ccTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCaaa                                                                                                                               >  2:862731/1‑71 (MQ=255)
                                                                                                  cTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTgg                                                                                       <  1:402435/110‑1 (MQ=255)
                                                                                                  cTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTgg                                                                                       >  2:402435/1‑110 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCTGGTGTTCt                                                                                                                                              <  2:1840690/54‑1 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCTGGTGTTCt                                                                                                                                              >  1:1840690/1‑54 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCt                                                                                           >  2:505475/1‑105 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCt                                                                                           >  4:1641184/1‑105 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTgg                                                                                       <  2:1055719/109‑1 (MQ=255)
                                                                                                   tAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGc                                               <  3:1838894/149‑1 (MQ=255)
                                                                                                    aaaaaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTgga                                                                                                         <  2:1665118/90‑1 (MQ=255)
                                                                                                    aaaaaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTgga                                                                                                         >  1:1665118/1‑90 (MQ=255)
                                                                                                    aaaaaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGCCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCt                                              <  1:1881582/149‑1 (MQ=255)
                                                                                                       aaaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTg                                                                                        >  1:156670/1‑104 (MQ=255)
                                                                                                         aCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCAc                                         >  4:21256/1‑149 (MQ=255)
                                                                                                           aTGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCt                                       >  3:1487517/1‑149 (MQ=255)
                                                                                                              ttCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAttt                                                                                 >  3:678573/1‑104 (MQ=255)
                                                                                                              ttCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCa                                    <  3:230228/149‑1 (MQ=255)
                                                                                                               tCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAg                                   >  4:125160/1‑149 (MQ=255)
                                                                                                                cATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAgg                                  <  3:705155/149‑1 (MQ=255)
                                                                                                                 aTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                                       >  2:1352279/1‑111 (MQ=255)
                                                                                                                 aTGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                                       <  1:1352279/111‑1 (MQ=255)
                                                                                                                   gCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCt                               >  3:78585/1‑149 (MQ=255)
                                                                                                                    ccTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCtt                              >  4:408622/1‑149 (MQ=255)
                                                                                                                    ccTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCtt                              <  4:1487517/149‑1 (MQ=255)
                                                                                                                     cTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTg                             >  2:1702871/1‑149 (MQ=255)
                                                                                                                       ggCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTc                           <  2:1049393/149‑1 (MQ=255)
                                                                                                                        gCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGa                                                                                                                             >  1:260589/1‑50 (MQ=255)
                                                                                                                        gCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGa                                                                                                                             <  2:260589/50‑1 (MQ=255)
                                                                                                                        gCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTcc                          >  1:821031/1‑149 (MQ=255)
                                                                                                                            ggCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACa                      >  2:261096/1‑149 (MQ=255)
                                                                                                                               cTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTg                                                                                        >  3:1818379/1‑80 (MQ=255)
                                                                                                                               cTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTg                                                                                        <  4:1818379/80‑1 (MQ=255)
                                                                                                                               cTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTc                                           <  4:309227/125‑1 (MQ=255)
                                                                                                                                tCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCa                  >  3:46637/1‑149 (MQ=255)
                                                                                                                                  cGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTc                           <  1:1387577/138‑1 (MQ=255)
                                                                                                                                    tttCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGc              >  2:1764415/1‑149 (MQ=255)
                                                                                                                                       cAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGa           <  4:1652053/149‑1 (MQ=255)
                                                                                                                                        aGAACACCTGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAttt                                                                                 <  3:1365199/78‑1 (MQ=255)
                                                                                                                                        aGAACACCTGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGAttt                                                                                 >  4:1365199/1‑78 (MQ=255)
                                                                                                                                        aGAACACCTGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAggg                                 >  3:1219871/1‑126 (MQ=255)
                                                                                                                                        aGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCt                                                                               <  1:1179733/80‑1 (MQ=255)
                                                                                                                                        aGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGaa          <  4:65280/149‑1 (MQ=255)
                                                                                                                                        aGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGaa          >  1:1841112/1‑149 (MQ=255)
                                                                                                                                         gAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCaga                >  2:232830/1‑142 (MQ=255)
                                                                                                                                         gAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCaga                <  1:232830/142‑1 (MQ=255)
                                                                                                                                         gAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGaaa         >  2:1313968/1‑149 (MQ=255)
                                                                                                                                            cacCTGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGtt                                                        <  1:1647141/99‑1 (MQ=255)
                                                                                                                                            cacCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGt      <  1:607598/149‑1 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGt                                                                                      >  1:1074937/1‑66 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGt                                                                                      <  2:1074937/66‑1 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCAc                                         >  4:1798355/1‑111 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACa                      >  4:1415384/1‑130 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACa                      <  3:1415384/130‑1 (MQ=255)
                                                                                                                                               cAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAg   <  1:261096/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGA  >  NC_000913/1914702‑1914994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: