Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,981,815 | T→A | 6.2% | R191R (CGA→CGT) | otsB ← | trehalose‑6‑phosphate phosphatase, biosynthetic |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,981,815 | 0 | T | A | 6.2% | 405.3 / 14.0 | 113 | R191R (CGA→CGT) | otsB | trehalose‑6‑phosphate phosphatase, biosynthetic |
Reads supporting (aligned to +/- strand): ref base T (52/54); new base A (3/4); total (55/58) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01 |
Only 100 of 119 total aligned reads displayed.
AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAA > NC_000913/1981668‑1981961 | aaGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTc < 3:745290/149‑1 (MQ=255) aGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGTTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCg > 2:255944/1‑149 (MQ=255) aGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCg > 4:515212/1‑149 (MQ=255) tCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtat < 4:392096/149‑4 (MQ=255) cAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATaaa > 1:576583/1‑149 (MQ=255) cAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATaaa > 4:744469/1‑149 (MQ=255) cAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATaaa < 3:202894/149‑1 (MQ=255) aTCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg < 1:142615/149‑1 (MQ=255) cacaCCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCt < 2:576583/149‑1 (MQ=255) acCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGtt > 1:863472/1‑124 (MQ=255) acCCGCCAGTCGCCATGATGCATGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGtt < 1:554147/124‑1 (MQ=255) aGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGc > 4:259182/1‑149 (MQ=255) tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgt > 2:622154/1‑121 (MQ=255) tCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgt < 1:622154/122‑2 (MQ=255) ccATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa > 4:431039/1‑149 (MQ=255) atgatgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTTGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAAtt < 2:1546376/149‑1 (MQ=255) atgatgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGc < 2:452597/142‑1 (MQ=255) atgatgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGc > 1:452597/1‑142 (MQ=255) atgCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGcc < 2:1317154/149‑1 (MQ=255) ccTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCa > 1:556902/1‑149 (MQ=255) cTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc < 2:556902/149‑1 (MQ=255) cTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc > 3:16693/1‑149 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAcc < 1:255944/149‑1 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAcc < 3:259182/149‑1 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc < 3:921969/148‑1 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc > 4:921969/1‑148 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc < 1:1037531/148‑1 (MQ=255) tGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc > 2:1037531/1‑148 (MQ=255) tGAGTTGCACCGGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAcc > 1:1396705/1‑149 (MQ=255) ttGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGccc < 3:1019912/103‑1 (MQ=255) ttGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa < 4:16693/149‑1 (MQ=255) tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGc < 3:1846924/121‑1 (MQ=255) tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGc > 4:1846924/1‑121 (MQ=255) tGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTAc < 3:1357269/149‑1 (MQ=255) gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCAc > 4:1705438/1‑142 (MQ=255) gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACt < 3:431039/149‑1 (MQ=255) gCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACt < 4:1428745/149‑1 (MQ=255) tgtgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc > 4:1366409/1‑132 (MQ=255) tgtgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc < 3:1366409/132‑1 (MQ=255) tgCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACct > 1:49792/1‑149 (MQ=255) ccAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa > 3:453260/1‑102 (MQ=255) ccAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGa < 4:453260/102‑1 (MQ=255) ccAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACctct > 4:714111/1‑149 (MQ=255) ccAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACctct > 2:996875/1‑149 (MQ=255) cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa > 3:1605223/1‑116 (MQ=255) cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATaaaa < 4:1605223/116‑1 (MQ=255) cAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCaa > 4:1463822/1‑123 (MQ=255) aTTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCgg < 3:714111/149‑1 (MQ=255) cTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtc < 3:660795/149‑1 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAg > 1:224191/1‑92 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa > 2:833169/1‑103 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATa < 1:833169/103‑1 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt > 3:305164/1‑147 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt < 4:305164/147‑1 (MQ=255) tGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgtat > 2:350756/1‑82 (MQ=255) gACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc < 1:996875/149‑1 (MQ=255) aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt > 3:1106891/1‑140 (MQ=255) aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGt < 4:1106891/140‑1 (MQ=255) aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc < 2:115791/148‑1 (MQ=255) aCATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtctc > 1:115791/1‑148 (MQ=255) cATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGa < 3:515212/149‑1 (MQ=255) aTTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGac > 1:1616621/1‑149 (MQ=255) ttCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGAACGCCCgt > 4:665605/1‑77 (MQ=255) gTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCct < 3:744469/149‑1 (MQ=255) aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa < 1:1027805/105‑1 (MQ=255) aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTa > 2:1027805/1‑105 (MQ=255) aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaaca < 2:1849045/135‑1 (MQ=255) aCGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaaca > 1:1849045/1‑135 (MQ=255) cTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAAc < 2:49792/149‑1 (MQ=255) gAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc > 3:58003/1‑87 (MQ=255) gAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGc < 4:58003/87‑1 (MQ=255) gAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAtct < 3:1566788/119‑1 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa > 3:1638475/1‑119 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa < 4:1638475/119‑1 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTaa < 2:129568/138‑1 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCAt > 3:1624698/1‑144 (MQ=255) aGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCAt < 4:1624698/144‑1 (MQ=255) ttCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg < 1:1877237/146‑1 (MQ=255) ttCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTtgtg > 2:1877237/1‑146 (MQ=255) aTCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACg > 3:1782096/1‑134 (MQ=255) aTCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACg < 4:1782096/134‑1 (MQ=255) tCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa > 1:1271108/1‑112 (MQ=255) tCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGacaa < 2:1271108/112‑1 (MQ=255) ggTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCt > 4:1657779/1‑149 (MQ=255) ggTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCt > 4:709434/1‑149 (MQ=255) gTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTg > 2:304855/1‑149 (MQ=255) gTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTg > 2:1481139/1‑149 (MQ=255) tAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAg < 1:304855/149‑1 (MQ=255) aaaTCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt > 1:1225581/1‑98 (MQ=255) aaaTCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGAt < 2:1225581/98‑1 (MQ=255) tcGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACg > 4:1075703/1‑149 (MQ=255) cAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATc > 1:1062551/1‑133 (MQ=255) aGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGa > 2:251252/1‑85 (MQ=255) aGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGa < 1:251252/85‑1 (MQ=255) aTACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAAt < 3:709434/149‑1 (MQ=255) tACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATg > 4:256473/1‑149 (MQ=255) gCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCAtt < 2:688916/149‑1 (MQ=255) gTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTaa < 3:256473/149‑1 (MQ=255) gTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTaa > 1:1871752/1‑149 (MQ=255) ttCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCctgc < 2:1876407/95‑1 (MQ=255) | AAGCCAGCTCCAGACATCCGGCACACCCGCCAGTCGCCATGATGCCTGAGTTGCACCTGTGCCAATTTTTACTGACATTCCGCCCAGTCGGTTAACGACTGCGAAGCCAGATTCATCGGTTAAATCATCGCCCAGAAATACGGGCGTTCGCCCGATAAAGGGAGCTTCCTGCATAAAAGCTGCAATTGCCTCACCTTTACTGGTACCTCTCGGTTTGATCTCGACAACACACTTTCCCTGCTGTAACGCCATTTGTGGCCAGATCTGAGTAATACGTTGCGCTAATGTCATTAA > NC_000913/1981668‑1981961 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |