Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,991,866 T→A 10.2% noncoding (36/87 nt) leuZ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,991,8660TA10.2% 324.9 / 23.5 98noncoding (36/87 nt)leuZtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (47/41);  new base A (5/5);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 106 total aligned reads displayed.

GTAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTC  >  NC_000913/1991727‑1992005
                                                                                                                                           |                                                                                                                                           
gtAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCtt                                                                                                                                    <  2:1859781/149‑1 (MQ=255)
 tAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtcg                                                                                                                                   <  3:1780189/149‑3 (MQ=255)
     gACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTc                                                                                                                               >  2:182421/1‑149 (MQ=255)
               aGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGAt                                                                                                                        <  1:1042630/146‑1 (MQ=255)
               aGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGAt                                                                                                                        >  2:1042630/1‑146 (MQ=255)
                   acaAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccac                                                                                                                   <  2:155018/147‑1 (MQ=255)
                   acaAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccac                                                                                                                   >  1:155018/1‑147 (MQ=255)
                     aaaaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTaaa                                                                                                                                          <  4:1538998/122‑1 (MQ=255)
                     aaaaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTaaa                                                                                                                                          >  3:1538998/1‑122 (MQ=255)
                       aaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCg                                                                                                             >  4:1681050/1‑149 (MQ=255)
                            ccaccCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTc                                                                                                        >  1:696513/1‑149 (MQ=255)
                              accCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCgg                                                                                                      >  1:391297/1‑149 (MQ=255)
                              accCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCgg                                                                                                      >  1:372169/1‑149 (MQ=255)
                                ccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                     <  4:1000781/132‑1 (MQ=255)
                                ccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                     >  3:1000781/1‑132 (MQ=255)
                                ccGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGga                                                                                                    >  3:621077/1‑149 (MQ=255)
                                 cGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaa                                                                                                   <  3:1681050/149‑1 (MQ=255)
                                 cGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaa                                                                                                   <  2:391297/149‑1 (MQ=255)
                                      gtggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAg                                                                                              >  1:1692640/1‑149 (MQ=255)
                                        ggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTg                                                                                            <  1:182421/149‑1 (MQ=255)
                                          tttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa                                                                                          <  2:696513/149‑1 (MQ=255)
                                              aCGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGc                                                                                                          >  3:1568073/1‑129 (MQ=255)
                                               cGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                     <  1:879020/149‑1 (MQ=255)
                                                     tGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg                                                                                           >  4:677420/1‑137 (MQ=255)
                                                     tGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg                                                                                           <  3:677420/137‑1 (MQ=255)
                                                         tATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGt                                                                           >  4:90899/1‑149 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccac                                                                                                                   <  3:686728/103‑1 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTccac                                                                                                                   >  4:686728/1‑103 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                     <  1:554477/101‑1 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTcc                                                                                                                     >  2:554477/1‑101 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa                                                                                          >  4:907443/1‑128 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa                                                                                          <  3:907443/128‑1 (MQ=255)
                                                               tttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                     >  2:783541/1‑149 (MQ=255)
                                                                  gATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                  <  3:81285/149‑1 (MQ=255)
                                                                    ttttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                       >  4:520735/1‑126 (MQ=255)
                                                                    ttttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                       <  3:520735/126‑1 (MQ=255)
                                                                    ttttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACt                                                                >  2:614608/1‑149 (MQ=255)
                                                                    ttttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACt                                                                <  4:1000059/149‑1 (MQ=255)
                                                                     tttATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTa                                                               >  2:1579233/1‑149 (MQ=255)
                                                                            ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                     <  1:784265/120‑1 (MQ=255)
                                                                            ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                     >  2:784265/1‑120 (MQ=255)
                                                                            ttATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGAt                                                        >  3:249569/1‑149 (MQ=255)
                                                                               tCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTg                                                     <  1:783541/149‑1 (MQ=255)
                                                                                 tttCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                   >  4:1760216/1‑149 (MQ=255)
                                                                                 tttCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa                                                   >  2:983807/1‑149 (MQ=255)
                                                                                  ttCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCaa                                                  <  1:614608/149‑1 (MQ=255)
                                                                                     ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt                                                                                             <  1:1587270/103‑1 (MQ=255)
                                                                                     ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt                                                                                             >  2:1587270/1‑103 (MQ=255)
                                                                                     ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATcc                                               >  3:255596/1‑149 (MQ=255)
                                                                                     ccATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATcc                                               >  4:808049/1‑149 (MQ=255)
                                                                                       aTGGTGCCCGGAGCGGGACTTGAACCAGCACAGCGCGGACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGc                                             <  2:372169/149‑1 (MQ=255)
                                                                                         ggTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTa                                           <  4:621077/149‑1 (MQ=255)
                                                                                         ggTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTa                                           <  1:1579233/149‑1 (MQ=255)
                                                                                             cccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGaaa                                                                                               >  2:819364/1‑93 (MQ=255)
                                                                                             cccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGaaa                                                                                               <  1:819364/93‑1 (MQ=255)
                                                                                             cccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATa                                       >  1:1619590/1‑149 (MQ=255)
                                                                                              ccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATaa                                      >  1:202601/1‑149 (MQ=255)
                                                                                              ccGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATaa                                      >  3:852583/1‑149 (MQ=255)
                                                                                                   gCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATaa                                      >  4:1032681/1‑144 (MQ=255)
                                                                                                   gCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATaa                                      <  3:1032681/144‑1 (MQ=255)
                                                                                                    cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                <  3:1760216/149‑1 (MQ=255)
                                                                                                    cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                <  2:202601/149‑1 (MQ=255)
                                                                                                    cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                <  4:852583/149‑1 (MQ=255)
                                                                                                    cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                <  3:808049/149‑1 (MQ=255)
                                                                                                    cGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt                                <  1:983807/149‑1 (MQ=255)
                                                                                                       gACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGt                             >  3:506889/1‑149 (MQ=255)
                                                                                                            gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAAc                                     >  1:1590582/1‑136 (MQ=255)
                                                                                                            gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAAc                                     <  2:1590582/136‑1 (MQ=255)
                                                                                                            gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcg                        >  4:303137/1‑149 (MQ=255)
                                                                                                            gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcg                        >  4:23706/1‑149 (MQ=255)
                                                                                                            gAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcg                        >  1:33564/1‑149 (MQ=255)
                                                                                                                       gcgcGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct             >  3:1781494/1‑149 (MQ=255)
                                                                                                                           gAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct             <  4:18251/145‑1 (MQ=255)
                                                                                                                           gAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAAttct             >  3:18251/1‑145 (MQ=255)
                                                                                                                           gAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAg         <  4:249569/149‑1 (MQ=255)
                                                                                                                             aCGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                     >  4:1319167/1‑71 (MQ=255)
                                                                                                                             aCGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgcgc                                                                                     <  3:1319167/71‑1 (MQ=255)
                                                                                                                               gCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCt     <  2:33564/149‑1 (MQ=255)
                                                                                                                                ccGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                       >  1:350883/1‑66 (MQ=255)
                                                                                                                                ccGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGgc                                                                                       <  2:350883/66‑1 (MQ=255)
                                                                                                                                 caaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTCCATAACCGCttt                               <  3:138345/119‑1 (MQ=255)
                                                                                                                                 caaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                               <  4:534239/119‑1 (MQ=255)
                                                                                                                                 caaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCttt                               >  4:138345/3‑121 (MQ=255)
                                                                                                                                 cGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCttt   >  1:1830182/1‑149 (MQ=255)
                                                                                                                                  gAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc  <  4:506889/149‑1 (MQ=255)
                                                                                                                                  gAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc  <  3:909068/149‑1 (MQ=255)
                                                                                                                                  gAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc  >  3:782458/1‑149 (MQ=255)
                                                                                                                                  aaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                     >  1:1045552/2‑82 (MQ=255)
                                                                                                                                  aaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAAcc                                                                     <  2:1045552/81‑1 (MQ=255)
                                                                                                                                  aaGGGATTTAAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTc  >  2:140485/2‑149 (MQ=255)
                                                                                                                                  aaGGGATTTAAAATCCCTCGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                  >  1:1455220/2‑85 (MQ=255)
                                                                                                                                  aaGGGATTTAAAATCCCTCGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGa                                                                  <  2:1455220/84‑1 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                             <  2:639372/68‑1 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGa                                                                             >  1:639372/1‑68 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGc                                                    >  4:1465529/1‑93 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGc                                                    <  3:1465529/93‑1 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCaaa                 >  1:1800872/1‑128 (MQ=255)
                                                                                                                                        ttttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCaaa                 <  2:1800872/128‑1 (MQ=255)
                                                                                                                                          ttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAATGTGGAGGCGCGTTCCGGAGTc                                                                          >  4:1396349/1‑69 (MQ=255)
                                                                                                                                          ttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTc                                                                          <  3:1396349/69‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTAACGACAAGTTGCAGGCACAAAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTC  >  NC_000913/1991727‑1992005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: