Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,992,083 G→C 6.8% noncoding (35/76 nt) glyW ← tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,992,0830GC 6.8% 579.8 / 27.6 161noncoding (35/76 nt)glyWtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (70/80);  new base C (5/6);  total (75/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-02

Only 100 of 166 total aligned reads displayed.

AACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCA  >  NC_000913/1991935‑1992230
                                                                                                                                                    |                                                                                                                                                   
aaCCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                     <  2:1821847/149‑1 (MQ=255)
        tAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCg                                                                                                                                             <  1:1795903/149‑1 (MQ=255)
          gACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                           >  3:1632598/1‑149 (MQ=255)
          gACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgg                                                                                                                                            >  2:1178448/1‑147 (MQ=255)
          gACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgg                                                                                                                                            <  1:1178448/148‑2 (MQ=255)
           aCGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                                                                                          <  3:1011198/149‑1 (MQ=255)
             ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                        <  1:344966/149‑1 (MQ=255)
             ggATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                                        >  4:1004141/1‑149 (MQ=255)
              gATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                                                                                       <  2:32482/149‑1 (MQ=255)
                tttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                     >  4:1359385/1‑149 (MQ=255)
                tttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                     >  2:218928/1‑149 (MQ=255)
                    cAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc                                                                                                                                 <  1:120781/149‑1 (MQ=255)
                     aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt                                                                                                                                >  3:405036/1‑149 (MQ=255)
                      aTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                                                                                       <  1:1175431/141‑1 (MQ=255)
                      aTCCGCTACATAACCGCTTCGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctct                                                                                                                                       >  2:1845712/1‑141 (MQ=255)
                         cGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc                                                                                                                            >  1:1653741/1‑149 (MQ=255)
                             aCATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAtt                                                                                                                        <  3:345372/149‑1 (MQ=255)
                              cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                                                                                       <  4:405036/149‑1 (MQ=255)
                              cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                                                                                       >  4:1579908/1‑149 (MQ=255)
                                 aaCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCg                                                                                                                    >  3:811727/1‑149 (MQ=255)
                                    cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAt                                                                                                                 >  2:1687526/1‑149 (MQ=255)
                                    cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAt                                                                                                                 >  2:614079/1‑149 (MQ=255)
                                     gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAtt                                                                                                                >  3:336187/1‑149 (MQ=255)
                                           ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaa                                                                                                          <  1:218928/149‑1 (MQ=255)
                                           ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaa                                                                                                          <  2:1653741/149‑1 (MQ=255)
                                           ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaa                                                                                                          <  4:1632598/149‑1 (MQ=255)
                                            tAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCaat                                                                                                         <  4:1262227/149‑2 (MQ=255)
                                                  gcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCa                                                                                                   <  3:1359385/149‑1 (MQ=255)
                                                   ccAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAg                                                                                                  >  1:275848/1‑149 (MQ=255)
                                                     aaaTTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGtt                                                                                                <  1:614079/149‑1 (MQ=255)
                                                       aTTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                          >  2:1702291/1‑121 (MQ=255)
                                                        ttcttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                          >  4:1556147/1‑120 (MQ=255)
                                                        ttcttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                          <  3:1556147/120‑1 (MQ=255)
                                                        ttcttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                          <  1:1734847/120‑1 (MQ=255)
                                                        ttcttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                             >  2:82040/1‑133 (MQ=255)
                                                        ttcttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                             <  1:82040/133‑1 (MQ=255)
                                                           ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                          <  2:617270/149‑1 (MQ=255)
                                                           ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                          >  1:1043954/1‑149 (MQ=255)
                                                           ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                          <  2:1420409/149‑1 (MQ=255)
                                                             cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACtt                                                                                        <  1:1404935/149‑1 (MQ=255)
                                                             cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACtt                                                                                        >  3:1534560/1‑149 (MQ=255)
                                                               ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGa                                                                                      >  3:24525/1‑149 (MQ=255)
                                                               ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGa                                                                                      <  3:1579908/149‑1 (MQ=255)
                                                                  cTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAttt                                                                                   <  4:747843/149‑1 (MQ=255)
                                                                   tttCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAtttt                                                                                  >  3:42951/1‑149 (MQ=255)
                                                                       aGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                         <  1:68912/122‑1 (MQ=255)
                                                                       aGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                         >  2:68912/1‑122 (MQ=255)
                                                                         ccAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCa                                                                                                   >  4:681023/1‑126 (MQ=255)
                                                                         ccAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCa                                                                                                   <  3:681023/126‑1 (MQ=255)
                                                                         ccAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                            <  4:1534560/149‑1 (MQ=255)
                                                                           aGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                            >  1:1028476/1‑147 (MQ=25)
                                                                           aGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                            <  2:1028476/147‑1 (MQ=25)
                                                                              cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTg                                                                       <  1:1101209/149‑1 (MQ=255)
                                                                              cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTg                                                                       >  1:967200/1‑149 (MQ=255)
                                                                              cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTg                                                                       <  1:1546907/149‑1 (MQ=255)
                                                                               aTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTgg                                                                      <  2:967200/149‑1 (MQ=255)
                                                                               aTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTgg                                                                      <  3:761221/149‑1 (MQ=255)
                                                                               aTCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTgg                                                                      >  4:857795/1‑149 (MQ=255)
                                                                                   gCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACtt                                                                                        <  2:935548/127‑1 (MQ=255)
                                                                                   gCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAAt                                                                  >  4:1068249/1‑149 (MQ=255)
                                                                                       gACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg                                                                                                                                           <  4:388942/72‑3 (MQ=255)
                                                                                        aCGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTg                                                             <  1:1687526/149‑1 (MQ=255)
                                                                                          gCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttat                                                                                <  2:1074215/128‑1 (MQ=255)
                                                                                           ccGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccg                                                          <  3:857795/149‑1 (MQ=255)
                                                                                               tGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGt                                                      <  4:319852/149‑1 (MQ=255)
                                                                                                 tCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAg                                                                                                  <  1:1065295/103‑1 (MQ=255)
                                                                                                 tCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAg                                                                                                  >  2:1065295/1‑103 (MQ=255)
                                                                                                   ttttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCt                                                                                                                           <  2:360618/76‑1 (MQ=255)
                                                                                                   ttttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGa                                                  >  3:900553/1‑149 (MQ=255)
                                                                                                      tAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGc                                               <  1:905830/149‑1 (MQ=255)
                                                                                                             gAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCAt                                        <  2:1382649/149‑1 (MQ=255)
                                                                                                              aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCAtt                                       <  3:1068249/149‑1 (MQ=255)
                                                                                                                     aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGt                                                      <  3:1023065/127‑1 (MQ=255)
                                                                                                                     aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGt                                                      >  4:1023065/1‑127 (MQ=255)
                                                                                                                        gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                             <  2:888140/69‑1 (MQ=40)
                                                                                                                        gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAAt                                                                                             <  3:771329/85‑1 (MQ=255)
                                                                                                                        gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAAt                                                                                             >  4:771329/1‑85 (MQ=255)
                                                                                                                         agaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTg                            >  2:1622570/1‑149 (MQ=255)
                                                                                                                          gaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCAtt                                                                                                                >  1:1064856/1‑64 (MQ=35)
                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAAt                                                                                                     <  1:790802/72‑1 (MQ=255)
                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTa                                    <  1:608077/137‑1 (MQ=255)
                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTa                                    <  4:1832053/137‑1 (MQ=255)
                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTa                                    >  3:1832053/1‑137 (MQ=255)
                                                                                                                                aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt                     <  1:579767/149‑1 (MQ=255)
                                                                                                                                  cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCa                                         >  2:130496/1‑127 (MQ=255)
                                                                                                                                  cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCa                                         <  1:130496/127‑1 (MQ=255)
                                                                                                                                  cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa                   <  4:900553/149‑1 (MQ=255)
                                                                                                                                  cTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa                   >  2:1558961/1‑149 (MQ=255)
                                                                                                                                      agagcacGACCTTGCCAAGGTCGGGGTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa                   <  2:592861/139‑1 (MQ=255)
                                                                                                                                          cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTa                                    <  4:1339548/124‑1 (MQ=255)
                                                                                                                                          cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa           >  3:519890/1‑149 (MQ=255)
                                                                                                                                          cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa                   <  3:1475798/139‑1 (MQ=255)
                                                                                                                                          cacGACCTTGCCAACGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgc                                                            >  3:131312/3‑100 (MQ=255)
                                                                                                                                           ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGt                                                              >  2:257592/1‑97 (MQ=255)
                                                                                                                                           ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGt                 <  4:850106/142‑1 (MQ=255)
                                                                                                                                             gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCt                                                                        <  4:1702515/85‑1 (MQ=255)
                                                                                                                                             gACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCt                                                                        >  3:1702515/1‑85 (MQ=255)
                                                                                                                                                   ggCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca  <  4:519890/149‑1 (MQ=255)
                                                                                                                                                   ggCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca  <  2:1059865/149‑1 (MQ=255)
                                                                                                                                                   ggCAAGGTCGTGCTCTACAAACTGAGCTATTCCCGCATTCATCAAGCAATCCGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTCCTTACCTGGCGCGATGAGTCAACGATATTTTTca  <  1:1643712/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCA  >  NC_000913/1991935‑1992230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: