Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,025,579 T→C 5.2% S23S (AGT→AGC) ‡ yedP → putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,5790TC 5.2% 535.0 / 12.0 153S23S (AGT→AGC) ‡yedPputative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (76/69);  new base C (4/4);  total (80/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 157 total aligned reads displayed.

CGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATG  >  NC_000913/2025436‑2025727
                                                                                                                                               |                                                                                                                                                    
   tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTa                                                                                                                                                    >  1:960399/1‑143 (MQ=255)
   tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTa                                                                                                                                                    <  2:960399/143‑1 (MQ=255)
   tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGa                                                                                                                                                 <  3:546543/146‑1 (MQ=255)
   tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              <  4:1689642/149‑1 (MQ=255)
   tCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              >  3:1689642/1‑149 (MQ=255)
              tcatcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAg                                                                                                                                   >  1:1370818/1‑149 (MQ=255)
               catcTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGc                                                                                                                                  >  3:74876/1‑149 (MQ=255)
                   tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTgg                                                                                                                                             <  3:245320/134‑1 (MQ=255)
                   tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTgg                                                                                                                                             >  4:245320/1‑134 (MQ=255)
                   tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGccccc                                                                                                                              >  4:572504/1‑149 (MQ=255)
                   tttGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACt                                                                                                                                               <  2:986836/132‑1 (MQ=255)
                         aaTTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              <  2:1824799/127‑1 (MQ=255)
                         aaTTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              <  4:49040/127‑1 (MQ=255)
                         aaTTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTg                                                                                                                                              >  3:49040/1‑127 (MQ=255)
                               tgCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGt                                                                                                                  >  2:445962/1‑149 (MQ=255)
                                    ttttGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAcg                                                                                                             >  2:378148/1‑149 (MQ=255)
                                     tttGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAcgc                                                                                                            <  4:1840706/149‑1 (MQ=255)
                                        gagaATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         >  3:1519333/1‑149 (MQ=255)
                                        gagaATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         <  1:445962/149‑1 (MQ=255)
                                           aaTCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         >  4:1766075/1‑146 (MQ=255)
                                            atcatcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCaa                                                                                                     >  1:307905/1‑149 (MQ=255)
                                              catcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAAt                                                                                                   <  3:1087426/149‑1 (MQ=255)
                                               atcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATg                                                                                                  >  4:924281/1‑149 (MQ=255)
                                                tcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         <  4:1768415/141‑1 (MQ=255)
                                                tcCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         >  3:1768415/1‑141 (MQ=255)
                                                  cTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGc                                                                                                       >  3:1552878/1‑141 (MQ=255)
                                                  cTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACTgt                                                                                                                                             <  1:1312085/103‑2 (MQ=255)
                                                  cTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAACTATGACTgt                                                                                                                                             >  2:1312085/1‑102 (MQ=255)
                                                   tGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTcc                                                                                              <  4:733886/149‑1 (MQ=255)
                                                    ggTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTccc                                                                                             >  4:380572/1‑149 (MQ=255)
                                                      tAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATGTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGt                                                                                           >  1:1857744/1‑149 (MQ=255)
                                                      tAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGt                                                                                           >  3:913884/1‑149 (MQ=255)
                                                       aaGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAAt                                                                                                   >  4:185531/1‑140 (MQ=255)
                                                         gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         <  4:949949/132‑1 (MQ=255)
                                                         gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGaa                                                                                                         >  3:949949/1‑132 (MQ=255)
                                                         gaggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAt                                                                                        >  3:945387/1‑149 (MQ=255)
                                                          aggagTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAtg                                                                                       >  4:1013917/1‑148 (MQ=255)
                                                                  tGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAg                                                                               >  2:1150038/1‑149 (MQ=255)
                                                                   gcgcGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCt                                                                                                                             <  4:1163480/102‑1 (MQ=255)
                                                                   gcgcGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCt                                                                                                                             >  3:1163480/1‑102 (MQ=255)
                                                                   gcgcGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGc                                                                              >  3:779609/1‑149 (MQ=255)
                                                                    cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                             <  2:307905/149‑1 (MQ=255)
                                                                    cgcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCa                                                                             <  1:728830/149‑1 (MQ=255)
                                                                     gcgACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAg                                                                            >  1:1808405/1‑149 (MQ=255)
                                                                       gACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAAt                                                                                                   >  2:519112/1‑124 (MQ=255)
                                                                       gACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAAt                                                                                                   <  1:519112/124‑1 (MQ=255)
                                                                         cTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCa                                                                                         >  2:208426/1‑132 (MQ=255)
                                                                          tATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCaaa                                                                                                    <  4:410111/120‑1 (MQ=255)
                                                                          tATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCaaa                                                                                                    >  3:410111/1‑120 (MQ=255)
                                                                                  caattcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTa                                                                                >  3:1692448/1‑132 (MQ=255)
                                                                                    attcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACAt                                                                    <  4:328970/142‑1 (MQ=255)
                                                                                    attcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACAt                                                                    >  3:328970/1‑142 (MQ=255)
                                                                                    attcaaCAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGa                                                             >  1:1224066/1‑149 (MQ=255)
                                                                                        aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTAc                                                                                                              <  4:1610623/96‑1 (MQ=255)
                                                                                        aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAAc                                                                      >  1:221617/1‑136 (MQ=255)
                                                                                        aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCa                                                                  <  3:1644890/140‑1 (MQ=255)
                                                                                        aacaacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATg                                                         <  1:1150038/149‑1 (MQ=255)
                                                                                           aacCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTg                                                      >  3:1883090/1‑149 (MQ=255)
                                                                                             ccACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTa                                                    <  4:74876/149‑1 (MQ=255)
                                                                                              cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTa                                                                          >  1:110724/1‑126 (MQ=255)
                                                                                              cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTa                                                                          <  4:1851045/126‑1 (MQ=255)
                                                                                              cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTa                                                                          <  2:110724/126‑1 (MQ=255)
                                                                                              cACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTa                                                                          >  3:1851045/1‑126 (MQ=255)
                                                                                                ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                                 <  3:380572/149‑1 (MQ=255)
                                                                                                ctactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACtt                                                 <  4:1397976/149‑1 (MQ=255)
                                                                                                 tactGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCaaa                                                                                                    >  2:1325915/1‑97 (MQ=255)
                                                                                                  actGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGc                                               >  2:199723/1‑149 (MQ=255)
                                                                                                   ctGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCa                                              >  2:22614/1‑149 (MQ=255)
                                                                                                    tGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAt                                                                                        <  1:1817329/106‑1 (MQ=255)
                                                                                                    tGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCAt                                                                                        >  2:1817329/1‑106 (MQ=255)
                                                                                                    tGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaa                                             <  1:199723/149‑1 (MQ=255)
                                                                                                        ttttAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCaaaaaa                                         >  1:167200/1‑149 (MQ=255)
                                                                                                             gCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTg                                    >  4:461009/1‑149 (MQ=255)
                                                                                                              cGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                                   <  4:913884/149‑1 (MQ=255)
                                                                                                               gATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTggg                                  <  3:790030/149‑1 (MQ=255)
                                                                                                               gATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTggg                                  <  3:461009/149‑1 (MQ=255)
                                                                                                                aTCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgggg                                 >  3:573551/1‑149 (MQ=255)
                                                                                                                    tGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGt                                      <  2:593823/140‑1 (MQ=255)
                                                                                                                    tGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGt                                      >  1:593823/1‑140 (MQ=255)
                                                                                                                    tGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGt                                      <  2:6973/140‑1 (MQ=255)
                                                                                                                      aTGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTgg                                   <  2:1663946/141‑1 (MQ=255)
                                                                                                                        ggCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAgg                         <  2:167200/149‑1 (MQ=255)
                                                                                                                           aCCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTa                                                    <  2:795038/119‑1 (MQ=255)
                                                                                                                           aCCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTa                                                    >  1:795038/1‑119 (MQ=255)
                                                                                                                             ccTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTAc                    <  1:22614/149‑1 (MQ=255)
                                                                                                                               tgctgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCg                  >  3:739183/1‑149 (MQ=255)
                                                                                                                                 ctgGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCt                <  4:758590/149‑1 (MQ=255)
                                                                                                                                     ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTctct                                                                                   >  4:1682807/2‑78 (MQ=255)
                                                                                                                                     ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGa              <  1:1016644/146‑1 (MQ=255)
                                                                                                                                     ccAGTCATAACTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGa              >  2:1016644/2‑147 (MQ=255)
                                                                                                                                        gTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGcc         <  4:1519333/149‑1 (MQ=255)
                                                                                                                                         tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCt                                                       >  4:729259/1‑102 (MQ=255)
                                                                                                                                         tCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCt                                                       <  3:729259/102‑1 (MQ=255)
                                                                                                                                           aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAAc                                                                      <  1:1478103/85‑1 (MQ=255)
                                                                                                                                           aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAAc                                                                      >  2:1478103/1‑85 (MQ=255)
                                                                                                                                           aTAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGt                        <  3:1853735/131‑1 (MQ=255)
                                                                                                                                            tAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGc                                                        >  1:112447/1‑98 (MQ=255)
                                                                                                                                            tAGTTATGACTGGCAACCGGCAGCCCCCGGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGc                                                        <  2:112447/98‑1 (MQ=255)
                                                                                                                                              gTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACaa                           <  3:1790700/125‑1 (MQ=255)
                                                                                                                                              gTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAAt   >  1:551827/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGCTCCCGAAAAAATCATCTTTGATAATTTGTGCGGTTTTGAGAATCATCCTGGTAAGAGGAGTACTGCGCGACTATGTTTTCAATTCAACAACCACTACTGGTTTTTAGCGATCTTGATGGCACCCTGCTGGACAGTCATAGTTATGACTGGCAACCGGCAGCCCCCTGGCTCACCCGTTTACGCGAAGCAAATGTTCCCGTCATTCTCTGTAGCAGTAAAACATCAGCGGAAATGCTGTACTTGCAAAAAACGTTGGGGCTACAAGGTTTACCGCTGATTGCCGAAAATG  >  NC_000913/2025436‑2025727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: