Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,050,006 C→G 5.8% T1690S (ACT→AGT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,050,0060CG 5.8% 495.0 / 15.5 137T1690S (ACT→AGT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (61/68);  new base G (4/4);  total (65/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 141 total aligned reads displayed.

ACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCA  >  NC_000913/2049858‑2050154
                                                                                                                                                    |                                                                                                                                                    
aCAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAc                                                                                                                                                      <  4:324557/149‑1 (MQ=255)
    ttAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGAc                                                                                                                                                  <  3:169275/149‑1 (MQ=255)
    ttAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGAc                                                                                                                                                  <  4:1664170/149‑1 (MQ=255)
        cGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGCtt                                                                                                                                              <  1:1767177/149‑2 (MQ=255)
             aCAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGc                                                                                                                                         >  4:1022023/1‑149 (MQ=255)
               aaTTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCaa                                                                                                                                       <  3:1349892/149‑1 (MQ=255)
                 ttATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAcc                                                                                                                                     >  2:213787/1‑149 (MQ=255)
                 ttATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAcc                                                                                                                                     >  4:1183848/1‑149 (MQ=255)
                       tgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAg                                                                                                                               >  3:204644/1‑149 (MQ=255)
                        gtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGCtt                                                                                                                                              >  3:1631925/1‑132 (MQ=255)
                        gtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGCtt                                                                                                                                              <  4:1631925/133‑2 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             >  4:1703956/1‑149 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             >  2:1197212/1‑149 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             >  2:1420346/1‑149 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             >  3:323383/1‑149 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             <  3:634466/149‑1 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             <  1:213787/149‑1 (MQ=255)
                         tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             <  4:475749/149‑1 (MQ=255)
                             tGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCa                                                                                                                         >  4:530961/1‑149 (MQ=255)
                               aTACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAc                                                                                                                                      <  4:1366401/134‑1 (MQ=255)
                                tACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGc                                                                                                                                                <  1:740640/123‑1 (MQ=255)
                                tACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGc                                                                                                                                                >  2:740640/1‑123 (MQ=255)
                                tACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaa                                                                                                                      >  3:43212/1‑149 (MQ=255)
                                 aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaa                                                                                                                     <  4:81341/149‑1 (MQ=255)
                                 aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaa                                                                                                                     >  3:1711733/1‑149 (MQ=255)
                                  cGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTACCCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    <  4:1711733/149‑1 (MQ=255)
                                  cGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    <  2:1698785/149‑1 (MQ=255)
                                  cGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    <  4:323383/149‑1 (MQ=255)
                                       cAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTaa                                                                                                               <  4:1467871/149‑1 (MQ=255)
                                         ggTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAtt                                                                                                             >  2:682534/1‑149 (MQ=255)
                                           tGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             <  3:224507/131‑1 (MQ=255)
                                           tGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGaa                                                                                                                             >  4:224507/1‑131 (MQ=255)
                                               tttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCcgcg                                                                                                       >  3:1716645/1‑149 (MQ=255)
                                               tttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCcgcg                                                                                                       >  1:1332575/1‑149 (MQ=255)
                                               tttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCcgcg                                                                                                       >  1:1181221/1‑149 (MQ=255)
                                                  gATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGaa                                                                                                    <  3:1022023/149‑1 (MQ=255)
                                                      gCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGTCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTc                                                                                                <  4:964499/149‑1 (MQ=255)
                                                       ccGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTcc                                                                                               >  1:1489139/1‑149 (MQ=255)
                                                         gATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    <  1:670148/126‑1 (MQ=255)
                                                         gATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    >  2:670148/1‑126 (MQ=255)
                                                           tGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCa                                                                                           <  2:1181221/149‑1 (MQ=255)
                                                              tGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGc                                                                                        <  2:1489139/149‑1 (MQ=255)
                                                              tGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGc                                                                                        <  1:1576510/149‑1 (MQ=255)
                                                                 tACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTaa                                                                                     <  1:1516239/149‑1 (MQ=255)
                                                                    cGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagca                                                                                  <  4:204644/149‑1 (MQ=255)
                                                                     gCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTa                                                                                                                                <  4:1422547/102‑1 (MQ=255)
                                                                     gCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTa                                                                                                                                >  3:1422547/1‑102 (MQ=255)
                                                                     gCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagcag                                                                                 >  3:186521/1‑149 (MQ=255)
                                                                       aaaaCTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAgcagca                                                                               >  4:1721492/1‑149 (MQ=255)
                                                                         aaCTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCAcc                                                                             >  1:1020814/1‑149 (MQ=255)
                                                                               cctcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGtt                                                                       <  3:530961/149‑1 (MQ=255)
                                                                               cctcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGtt                                                                       >  4:1018295/1‑149 (MQ=255)
                                                                               cctcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGtt                                                                       >  4:1423051/1‑149 (MQ=255)
                                                                                 tcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGCtt                                                                                                                                              >  4:663195/1‑75 (MQ=255)
                                                                                 tcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAGTGACGCtt                                                                                                                                              <  3:663195/76‑2 (MQ=255)
                                                                                 tcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagcagc                                                                                >  1:790643/1‑138 (MQ=255)
                                                                                 tcctTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCATCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagcagc                                                                                <  2:790643/138‑1 (MQ=255)
                                                                                    ttAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAAc                                                                  <  3:1799712/149‑1 (MQ=255)
                                                                                     tAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACg                                                                 <  1:450152/149‑1 (MQ=255)
                                                                                       aCCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGga                                                               <  3:1721492/149‑1 (MQ=255)
                                                                                       aCCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGga                                                               <  1:1420346/149‑1 (MQ=255)
                                                                                         ctctGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCTGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGgatg                                                             >  1:1144970/1‑149 (MQ=255)
                                                                                         ctctGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGgatg                                                             >  3:81832/1‑149 (MQ=255)
                                                                                         ctctGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGgatg                                                             >  2:866371/1‑149 (MQ=255)
                                                                                             gTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCg                                                         >  2:901053/1‑149 (MQ=255)
                                                                                             gTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCg                                                         >  4:1886977/1‑149 (MQ=255)
                                                                                              tataCTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagca                                                                                  <  2:1692836/123‑1 (MQ=255)
                                                                                              tataCTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAagca                                                                                  >  1:1692836/1‑123 (MQ=255)
                                                                                               ataCTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCggg                                                       <  1:682534/149‑1 (MQ=255)
                                                                                                taCTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCgggg                                                      >  4:1260251/1‑149 (MQ=255)
                                                                                                   tCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCa                                                                                           >  2:906690/1‑109 (MQ=255)
                                                                                                   tCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGtttt                                                   >  4:923210/1‑149 (MQ=255)
                                                                                                     aTTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCg                                                 >  2:417481/1‑149 (MQ=255)
                                                                                                             tcAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAAtt                                         >  3:563794/1‑149 (MQ=255)
                                                                                                             tcAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAAtt                                         >  1:55065/1‑149 (MQ=255)
                                                                                                                gCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCtt                                      <  4:43212/149‑1 (MQ=255)
                                                                                                                 cACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtg                                     <  4:186521/149‑1 (MQ=255)
                                                                                                                  aCGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCTGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    <  2:1144970/149‑1 (MQ=255)
                                                                                                                  aCGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    <  1:866371/149‑1 (MQ=255)
                                                                                                                  aCGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    <  3:1423051/149‑1 (MQ=255)
                                                                                                                      ccGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                                                                                                                    <  3:1039086/65‑1 (MQ=255)
                                                                                                                       cGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACaa                               <  3:923210/149‑1 (MQ=255)
                                                                                                                            gcgcAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAAc                                                                  >  3:1688763/1‑109 (MQ=255)
                                                                                                                               cAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAg                       <  1:901053/149‑1 (MQ=255)
                                                                                                                                aaCCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAgg                      >  3:900270/1‑149 (MQ=255)
                                                                                                                                aaCCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAgg                      >  1:265533/1‑149 (MQ=255)
                                                                                                                                  ccATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGACCCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTc                    <  2:55065/149‑1 (MQ=255)
                                                                                                                                      gACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    <  4:1753582/129‑1 (MQ=255)
                                                                                                                                      gACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    <  4:436939/129‑1 (MQ=255)
                                                                                                                                      gACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTtgt                                    >  3:436939/1‑129 (MQ=255)
                                                                                                                                        cGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTg              >  4:1861940/1‑149 (MQ=255)
                                                                                                                                          gttaGCGTCAGTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGaa            >  3:251665/4‑149 (MQ=255)
                                                                                                                                           cAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCAc                                                                              >  4:485863/1‑82 (MQ=255)
                                                                                                                                           cAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCAc                                                                              <  3:485863/82‑1 (MQ=255)
                                                                                                                                            aaGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGTATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGaaaa          <  3:1260251/149‑1 (MQ=255)
                                                                                                                                            aaGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGaaaa          <  3:402023/149‑1 (MQ=255)
                                                                                                                                                gTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGt      <  1:1208068/149‑1 (MQ=255)
                                                                                                                                                    cTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTc                    >  2:562561/1‑131 (MQ=255)
                                                                                                                                                    cTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTc                    <  1:562561/131‑1 (MQ=255)
                                                                                                                                                    cTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCa  >  4:352123/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCA  >  NC_000913/2049858‑2050154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: