Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,122 A→G 26.3% intergenic (+76/‑70) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1220AG26.3% 66.0 / 24.2 38intergenic (+76/‑70)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (14/14);  new base G (5/5);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATT  >  NC_000913/2089974‑2090262
                                                                                                                                                     |                                                                                                                                            
aTAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGa                                                                                                                                              <  4:15062/149‑1 (MQ=255)
  aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                                                            <  2:783886/149‑1 (MQ=255)
      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCt                                                                                                                                        >  2:1842239/1‑149 (MQ=255)
          acaGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                                                                    >  2:669987/1‑149 (MQ=255)
            agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                  <  1:1842239/149‑1 (MQ=255)
            agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                  <  1:669987/149‑1 (MQ=255)
             gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                                                                 <  1:617526/149‑1 (MQ=255)
                               gTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                                                                >  4:1687677/1‑132 (MQ=255)
                               gTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                                                                <  3:1687677/132‑1 (MQ=255)
                                   aaTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                                                   <  1:23733/125‑1 (MQ=255)
                                   aaTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                                                   >  2:23733/1‑125 (MQ=255)
                                     tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                         >  1:34472/1‑149 (MQ=255)
                                                ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                                                                                                          >  3:257299/1‑105 (MQ=255)
                                                ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                                                                                                          <  4:257299/105‑1 (MQ=255)
                                                      tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                                                   <  3:553839/106‑1 (MQ=255)
                                                      tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                                                                   >  4:553839/1‑106 (MQ=255)
                                                       caccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtt                                                                                                                             <  4:1268627/111‑1 (MQ=255)
                                                       caccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCtt                                                                                                                             >  3:1268627/1‑111 (MQ=255)
                                                          catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAATAAAAAAGCCCCCGGAAGGTGATCTTCCgg                                                                                                                                   <  4:314686/103‑1 (MQ=25)
                                                          catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCAATAAAAAAGCCCCCGGAAGGTGATCTTCCgg                                                                                                                                   >  3:314686/1‑103 (MQ=25)
                                                                           tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGa                                                                                                                                              <  4:1083108/74‑1 (MQ=255)
                                                                           tttCAGGCGATCTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGa                                                                                                                                              >  3:1083108/1‑74 (MQ=255)
                                                                                             ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                                                                >  1:1543055/1‑70 (MQ=255)
                                                                                             ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                                                                <  2:1543055/70‑1 (MQ=255)
                                                                                                     ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                 <  2:656355/61‑1 (MQ=255)
                                                                                                     ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                 >  1:656355/1‑61 (MQ=255)
                                                                                                       cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                 <  4:191210/59‑1 (MQ=255)
                                                                                                       cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                 >  3:191210/1‑59 (MQ=255)
                                                                                                                 aGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCgg                             <  2:34472/149‑1 (MQ=255)
                                                                                                                                            ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                      >  3:60092/1‑98 (MQ=39)
                                                                                                                                            ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                      <  4:60092/98‑1 (MQ=255)
                                                                                                                                             cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                                                     <  2:295267/82‑1 (MQ=38)
                                                                                                                                             cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                                                     >  1:295267/1‑82 (MQ=38)
                                                                                                                                             cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaaa                                  >  4:696860/1‑117 (MQ=255)
                                                                                                                                             cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaaa                                  <  3:696860/117‑1 (MQ=255)
                                                                                                                                             cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt  >  1:922341/1‑149 (MQ=255)
                                                                                                                                             cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt  >  3:1636631/1‑149 (MQ=255)
                                                                                                                                                 gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaa                                   <  2:230737/112‑1 (MQ=255)
                                                                                                                                                 gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGaa                                   >  1:230737/1‑112 (MQ=255)
                                                                                                                                                    gATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc             <  2:1549171/131‑1 (MQ=255)
                                                                                                                                                    gATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc             >  1:1549171/1‑131 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
ATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATT  >  NC_000913/2089974‑2090262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: