Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,165,143 C→T 5.1% intergenic (+145/‑46) baeR → / → yegP response regulator in two‑component regulatory system with BaeS/UPF0339 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,1430CT 5.1% 677.4 / 15.3 195intergenic (+145/‑46)baeR/yegPresponse regulator in two‑component regulatory system with BaeS/UPF0339 family protein
Reads supporting (aligned to +/- strand):  ref base C (99/86);  new base T (5/5);  total (104/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 197 total aligned reads displayed.

GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATA  >  NC_000913/2165006‑2165288
                                                                                                                                         |                                                                                                                                                 
gACATTATTTTGTTAGCCGGAGATGGAGTTCAGACCAGTTCCGGCAACATTATCCCACGCATGGTCGGCAAACTGACATTATCTCTCCGCCCTGGCAGTCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCt                                                                                                                                        >  3:1589863/1‑149 (MQ=255)
  cATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGt                                                                                                                                      >  2:1344646/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATCCTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                     <  1:1091541/149‑1 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCATCTAAAGGTGCTGtt                                                                                                                                     >  4:1204715/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                     >  4:1048409/1‑149 (MQ=255)
      attTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCATCTAAAGGTGCTGtttac                                                                                                                                  <  3:1204715/149‑3 (MQ=255)
        tttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                <  3:974250/149‑1 (MQ=255)
               gCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACaa                                                                                                                         <  3:1363814/149‑1 (MQ=255)
                        ggCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatca                                                                                                                >  3:760222/1‑149 (MQ=255)
                          cGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAg                                                                                                              <  4:905241/149‑1 (MQ=255)
                          cGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAg                                                                                                              <  3:198131/149‑1 (MQ=255)
                                 aCCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCAt                                                                                                       >  3:1295379/1‑149 (MQ=255)
                                    aGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAATTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    >  1:1349449/1‑149 (MQ=255)
                                           gCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggt                                                                                             >  4:326299/1‑149 (MQ=255)
                                             aaCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                           >  2:1364179/1‑149 (MQ=255)
                                              aCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttgg                                                                                          <  3:1152669/149‑1 (MQ=255)
                                                 ttATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTACATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGttt                                                                                       <  1:1212273/149‑1 (MQ=255)
                                                      ccACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtc                                                                                                                     <  1:921167/114‑1 (MQ=255)
                                                      ccACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtc                                                                                                                     >  2:921167/1‑114 (MQ=255)
                                                        aCGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  3:326299/149‑1 (MQ=255)
                                                         cGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                           <  3:368208/137‑1 (MQ=255)
                                                               gTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                <  4:753013/94‑1 (MQ=255)
                                                                tCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGc                                                                        >  4:1287148/1‑149 (MQ=255)
                                                                  aGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      <  1:1026990/149‑1 (MQ=255)
                                                                  aGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      >  2:1026990/1‑149 (MQ=255)
                                                                   gtttgcTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                     >  1:1580444/6‑149 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCttt                                                                                                                                               >  1:584538/1‑75 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCttt                                                                                                                                               <  2:584538/75‑1 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      >  2:25058/1‑148 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      >  1:866627/1‑148 (MQ=255)
                                                                          gACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                              >  4:338255/1‑149 (MQ=255)
                                                                            cATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAg                                                            <  3:1287148/149‑1 (MQ=255)
                                                                             aTTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCAc                                                                                                                                                   <  3:907935/61‑1 (MQ=255)
                                                                             aTTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtttt                                                                                                                                   >  4:1536236/1‑77 (MQ=255)
                                                                              ttATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGtt                                                          >  1:378390/1‑149 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGAt                                                                                                                               <  3:1768368/77‑1 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    >  1:723208/1‑120 (MQ=255)
                                                                                   tctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTTCgtt                                                     >  4:23891/1‑147 (MQ=255)
                                                                                       cGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                 >  3:338183/1‑149 (MQ=255)
                                                                                       cGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                 <  2:674116/149‑1 (MQ=255)
                                                                                        gCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACGCAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    <  4:1102874/113‑1 (MQ=255)
                                                                                         cccTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                              >  1:223619/1‑134 (MQ=255)
                                                                                         cccTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                              <  2:223619/134‑1 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTATATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGGAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTaa                                            >  1:1683421/1‑149 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                                                                                                            <  1:1760398/85‑1 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                                                                                                            >  2:1760398/1‑85 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTaa                                            <  4:1783520/149‑1 (MQ=255)
                                                                                                 ggcaTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCg                                       <  4:207186/149‑1 (MQ=255)
                                                                                                    aTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttggt                                                                                         <  3:598542/96‑1 (MQ=255)
                                                                                                    aTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttggt                                                                                         >  4:598542/1‑96 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    <  3:570357/100‑1 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    >  4:570357/1‑100 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               <  3:207278/71‑1 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCgg                                      <  3:1086263/144‑1 (MQ=255)
                                                                                                        gAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                <  2:378390/149‑1 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            >  4:1676024/1‑149 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            <  2:1580444/149‑1 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            >  2:1217786/1‑149 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTg                             <  4:1148130/148‑1 (MQ=255)
                                                                                                              cGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          <  4:1295379/149‑1 (MQ=255)
                                                                                                               gCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTc                                                         >  2:988215/1‑117 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  4:692875/92‑1 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  4:771170/92‑1 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGCAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  4:918139/92‑1 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          >  3:716465/1‑144 (MQ=255)
                                                                                                                    tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTACAAGCGGGCAATg                                >  3:1555082/1‑137 (MQ=255)
                                                                                                                    tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATcc                    >  1:631668/1‑149 (MQ=255)
                                                                                                                     aCTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCt                   >  1:1503471/1‑149 (MQ=255)
                                                                                                                      cTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTc                  >  4:1569054/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGt                                                                                                          <  2:877510/60‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            <  3:1800759/138‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:461109/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  1:1481007/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  2:859771/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  1:1414114/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 <  3:1505967/149‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:1399807/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:812551/1‑149 (MQ=255)
                                                                                                                        ttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCAc                <  2:1503471/149‑1 (MQ=255)
                                                                                                                          aaTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCa              >  1:1139120/1‑149 (MQ=255)
                                                                                                                           aTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCggg                                     >  4:1384548/1‑125 (MQ=255)
                                                                                                                           aTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGt                              <  4:1411055/132‑1 (MQ=255)
                                                                                                                             cGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGt                              <  2:1130915/130‑1 (MQ=255)
                                                                                                                               gaaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                     >  4:1218877/3‑89 (MQ=38)
                                                                                                                               gaaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                     <  3:1218877/87‑1 (MQ=38)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAg         >  3:1633104/1‑149 (MQ=255)
                                                                                                                                aaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          <  2:472587/130‑1 (MQ=255)
                                                                                                                                aaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          >  1:472587/2‑131 (MQ=255)
                                                                                                                                 aaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTACTAAAAGCGGGCAATGGTga                            >  2:1175881/1‑128 (MQ=255)
                                                                                                                                 aaaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                     >  4:1824106/1‑135 (MQ=255)
                                                                                                                                  aaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGt                              <  4:394638/125‑1 (MQ=255)
                                                                                                                                   aCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      <  2:1795477/84‑1 (MQ=39)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               >  3:644022/1‑43 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCttt     >  2:1735206/1‑149 (MQ=255)
                                                                                                                                    cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta    <  2:1481007/149‑1 (MQ=255)
                                                                                                                                    cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta    <  3:1117301/149‑1 (MQ=255)
                                                                                                                                     aGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                >  1:984771/1‑120 (MQ=255)
                                                                                                                                       cACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCa                                                             >  1:1337002/1‑89 (MQ=255)
                                                                                                                                        aCCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    >  4:398650/1‑49 (MQ=255)
                                                                                                                                        aCCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    <  3:398650/49‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATA  >  NC_000913/2165006‑2165288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: