Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,165,146 T→A 5.1% intergenic (+148/‑43) baeR → / → yegP response regulator in two‑component regulatory system with BaeS/UPF0339 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,1460TA 5.1% 715.9 / 18.9 195intergenic (+148/‑43)baeR/yegPresponse regulator in two‑component regulatory system with BaeS/UPF0339 family protein
Reads supporting (aligned to +/- strand):  ref base T (99/86);  new base A (5/5);  total (104/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 197 total aligned reads displayed.

GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATA  >  NC_000913/2165006‑2165288
                                                                                                                                            |                                                                                                                                              
gACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCt                                                                                                                                        >  4:549890/1‑149 (MQ=255)
gACATTATTTTGTTAGCCGGAGATGGAGTTCAGACCAGTTCCGGCAACATTATCCCACGCATGGTCGGCAAACTGACATTATCTCTCCGCCCTGGCAGTCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCt                                                                                                                                        >  3:1589863/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATCCTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                     <  1:1091541/149‑1 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                     >  2:1091541/1‑149 (MQ=255)
      attTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCATCTAAAGGTGCTGtttac                                                                                                                                  <  3:1204715/149‑3 (MQ=255)
       ttttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCg                                                                                                                                 >  2:429860/1‑149 (MQ=255)
        tttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGGAACATTCTCCCACGCATGGTCATCAAACTGACGTTATCTCTCCTCCCTGGCAGGCAGCAGAAAATCGCTCATACTTTAATCGTTAAAGAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                <  4:1589863/149‑1 (MQ=255)
            ttAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATaca                                                                                                                            >  2:1212273/1‑149 (MQ=255)
            ttAGCCGGAGATGGCGTTCAGACAAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCTCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAACACCTTTAGATGCTGTTTTCGATaca                                                                                                                            >  4:198131/1‑149 (MQ=255)
               gCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACaa                                                                                                                         <  3:1363814/149‑1 (MQ=255)
                 cGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAAtt                                                                                                                       >  1:674116/1‑149 (MQ=255)
                    agaTGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtca                                                                                                                    <  4:396266/149‑1 (MQ=255)
                        ggCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatca                                                                                                                >  3:760222/1‑149 (MQ=255)
                          cGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAg                                                                                                              <  3:198131/149‑1 (MQ=255)
                           gTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtc                                                                                                                     <  2:1588137/141‑1 (MQ=255)
                                 aCCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCAt                                                                                                       >  3:1295379/1‑149 (MQ=255)
                                    aGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    <  4:760222/149‑1 (MQ=255)
                                    aGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAATTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    >  1:1349449/1‑149 (MQ=255)
                                           gCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggt                                                                                             >  4:1217776/1‑149 (MQ=255)
                                             aaCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATa                                                                                                                              >  4:1158711/1‑114 (MQ=255)
                                             aaCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                           >  2:1472445/1‑149 (MQ=255)
                                              aCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttgg                                                                                          <  3:1152669/149‑1 (MQ=255)
                                                 ttATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTACATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGttt                                                                                       <  1:1212273/149‑1 (MQ=255)
                                                    tCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    <  2:1349449/149‑1 (MQ=255)
                                                      ccACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtc                                                                                                                     >  2:921167/1‑114 (MQ=255)
                                                      ccACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACt                                                                                  >  2:1490510/1‑149 (MQ=255)
                                                        aCGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  3:326299/149‑1 (MQ=255)
                                                         cGCATGGTCAGCAAACTGACATTATCTCTTCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                           >  4:368208/1‑137 (MQ=255)
                                                         cGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                           <  3:368208/137‑1 (MQ=255)
                                                            aTGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTggttggtt                                                                                        >  3:981898/1‑137 (MQ=255)
                                                                  aGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                      <  1:1026990/149‑1 (MQ=255)
                                                                   gtttgcTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                     >  1:1580444/6‑149 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCttt                                                                                                                                               >  1:584538/1‑75 (MQ=255)
                                                                       aCTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATa                                                                 >  4:1340851/1‑149 (MQ=255)
                                                                         tGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAAt                                                               >  2:589607/1‑149 (MQ=255)
                                                                            cATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAg                                                            <  3:1287148/149‑1 (MQ=255)
                                                                              ttATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGtt                                                          >  1:378390/1‑149 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGAt                                                                                                                               >  4:1768368/1‑77 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGAt                                                                                                                               <  3:1768368/77‑1 (MQ=255)
                                                                                     tcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGt                                                     <  2:1755677/147‑1 (MQ=255)
                                                                                       cGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                 >  3:338183/1‑149 (MQ=255)
                                                                                       cGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                 <  2:674116/149‑1 (MQ=255)
                                                                                         cccTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                              <  2:223619/134‑1 (MQ=255)
                                                                                         cccTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                              >  1:223619/1‑134 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTATATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGGAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTaa                                            >  1:1683421/1‑149 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                                                                                                            <  1:1760398/85‑1 (MQ=255)
                                                                                                  gcaTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATAAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                >  2:1533796/1‑107 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                    <  3:570357/100‑1 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCa                                   >  2:185749/1‑149 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               <  3:207278/71‑1 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               >  4:207278/1‑71 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCgg                                      <  3:1086263/144‑1 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCgg                                      >  4:1086263/1‑144 (MQ=255)
                                                                                                        gAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTggttggtt                                                                                        <  4:1290580/93‑1 (MQ=255)
                                                                                                        gAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                <  4:329981/149‑1 (MQ=255)
                                                                                                               gCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTc                                                         <  1:988215/117‑1 (MQ=255)
                                                                                                               gCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTc                                                         >  2:988215/1‑117 (MQ=255)
                                                                                                               gCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCggg                                     >  2:552723/1‑137 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                >  3:918139/1‑92 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTa                       >  1:947267/1‑149 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGACTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTa                       >  1:748366/1‑149 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGCAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  4:918139/92‑1 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          <  4:716465/144‑1 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          >  3:716465/1‑144 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                     >  4:1505967/1‑149 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                     >  2:1794701/1‑149 (MQ=255)
                                                                                                                    tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTACAAGCGGGCAATg                                >  3:1555082/1‑137 (MQ=255)
                                                                                                                    tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                <  4:1555082/137‑1 (MQ=255)
                                                                                                                     aCTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCt                   >  1:1503471/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGt                                                                                                          <  2:877510/60‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGt                                                                                                          >  1:877510/1‑60 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            <  3:1800759/138‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                            >  4:1800759/1‑138 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:812551/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  1:1414114/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 <  3:1505967/149‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  2:859771/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:1399807/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  3:461109/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                 >  1:1481007/1‑149 (MQ=255)
                                                                                                                          aaTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCa              >  1:1139120/1‑149 (MQ=255)
                                                                                                                             cGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCg           >  4:1117301/1‑149 (MQ=255)
                                                                                                                               gaaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                     <  3:1218877/87‑1 (MQ=38)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAg                                                            <  3:576442/98‑1 (MQ=255)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAg                                                            >  4:576442/1‑98 (MQ=255)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAg         >  3:1633104/1‑149 (MQ=255)
                                                                                                                                aaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                          >  1:472587/2‑131 (MQ=255)
                                                                                                                                  aaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCtt      <  4:1238246/149‑1 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               >  3:644022/1‑43 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                               <  4:644022/43‑1 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCttt     <  4:812551/149‑1 (MQ=255)
                                                                                                                                    cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta    <  3:1117301/149‑1 (MQ=255)
                                                                                                                                     aGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTtat   >  2:359895/1‑149 (MQ=255)
                                                                                                                                      gCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTtata  <  4:1399807/149‑1 (MQ=255)
                                                                                                                                      gCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTtata  <  2:1139120/149‑1 (MQ=255)
                                                                                                                                       cACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCa                                                             <  2:1337002/89‑1 (MQ=255)
                                                                                                                                       cACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCa                                                             >  1:1337002/1‑89 (MQ=255)
                                                                                                                                        aCCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                    <  3:398650/49‑1 (MQ=255)
                                                                                                                                        aCCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                <  4:259584/69‑1 (MQ=255)
                                                                                                                                          cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                 >  1:628735/1‑98 (MQ=255)
                                                                                                                                            |                                                                                                                                              
GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATA  >  NC_000913/2165006‑2165288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: