Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,165,147 T→A 5.1% intergenic (+149/‑42) baeR → / → yegP response regulator in two‑component regulatory system with BaeS/UPF0339 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,1470TA 5.1% 726.5 / 18.6 195intergenic (+149/‑42)baeR/yegPresponse regulator in two‑component regulatory system with BaeS/UPF0339 family protein
Reads supporting (aligned to +/- strand):  ref base T (99/86);  new base A (5/5);  total (104/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 198 total aligned reads displayed.

GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAA  >  NC_000913/2165006‑2165295
                                                                                                                                             |                                                                                                                                                    
gACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCt                                                                                                                                               >  4:549890/1‑149 (MQ=255)
gACATTATTTTGTTAGCCGGAGATGGAGTTCAGACCAGTTCCGGCAACATTATCCCACGCATGGTCGGCAAACTGACATTATCTCTCCGCCCTGGCAGTCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCt                                                                                                                                               >  3:1589863/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATCCTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                            <  1:1091541/149‑1 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCATCTAAAGGTGCTGtt                                                                                                                                            >  4:1204715/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                            >  4:1048409/1‑149 (MQ=255)
   attattTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtt                                                                                                                                            >  2:1091541/1‑149 (MQ=255)
        tttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                       <  3:974250/149‑1 (MQ=255)
        tttGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                       <  3:1048409/149‑1 (MQ=255)
            ttAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATaca                                                                                                                                   >  2:1212273/1‑149 (MQ=255)
               gCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACaa                                                                                                                                <  3:1363814/149‑1 (MQ=255)
                 cGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAAtt                                                                                                                              >  1:674116/1‑149 (MQ=255)
                  ggAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAAttt                                                                                                                             >  3:905241/1‑149 (MQ=255)
                    agaTGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtca                                                                                                                           <  4:396266/149‑1 (MQ=255)
                          cGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAg                                                                                                                     <  3:198131/149‑1 (MQ=255)
                           gTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtc                                                                                                                            <  2:1588137/141‑1 (MQ=255)
                                    aGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                           <  4:760222/149‑1 (MQ=255)
                                    aGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATAATTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                           >  1:1349449/1‑149 (MQ=255)
                                           gCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggt                                                                                                    >  4:326299/1‑149 (MQ=255)
                                             aaCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATa                                                                                                                                     <  3:1158711/114‑1 (MQ=255)
                                             aaCATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                                  >  2:1472445/1‑149 (MQ=255)
                                                 ttATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTACATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGttt                                                                                              <  1:1212273/149‑1 (MQ=255)
                                                    tCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                           <  2:1349449/149‑1 (MQ=255)
                                                      ccACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACt                                                                                         >  2:1490510/1‑149 (MQ=255)
                                                        aCGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       <  3:326299/149‑1 (MQ=255)
                                                        aCGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       <  3:1217776/149‑1 (MQ=255)
                                                         cGCATGGTCAGCAAACTGACATTATCTCTTCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                                  >  4:368208/1‑137 (MQ=255)
                                                         cGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttg                                                                                                  <  3:368208/137‑1 (MQ=255)
                                                               gTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGa                                                                                                                                       >  3:753013/1‑94 (MQ=255)
                                                                tCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGc                                                                               >  4:1287148/1‑149 (MQ=255)
                                                                  aGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                             <  1:1026990/149‑1 (MQ=255)
                                                                   gtttgcTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                            >  1:1580444/6‑149 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCttt                                                                                                                                                      >  1:584538/1‑75 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCttt                                                                                                                                                      <  2:584538/75‑1 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                             >  2:25058/1‑148 (MQ=255)
                                                                   gCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAg                                                                             <  2:866627/148‑1 (MQ=255)
                                                                             aTTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGtttt                                                                                                                                          >  4:1536236/1‑77 (MQ=255)
                                                                              ttATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGtt                                                                 >  1:378390/1‑149 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGAt                                                                                                                                      <  3:1768368/77‑1 (MQ=255)
                                                                                 tctctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                           <  2:723208/120‑1 (MQ=255)
                                                                                   tctcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTTCgtt                                                            >  4:23891/1‑147 (MQ=255)
                                                                                     tcCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGt                                                            <  2:1755677/147‑1 (MQ=255)
                                                                                       cGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                        >  3:338183/1‑149 (MQ=255)
                                                                                        gCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACGCAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                           <  4:1102874/113‑1 (MQ=255)
                                                                                        gCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                           >  3:1102874/1‑113 (MQ=255)
                                                                                         cccTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATc                                                                     >  1:223619/1‑134 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTATATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGGAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTaa                                                   >  1:1683421/1‑149 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGa                                                                                                                   <  1:1760398/85‑1 (MQ=255)
                                                                                            tGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTaa                                                   <  4:1783520/149‑1 (MQ=255)
                                                                                                 ggcaTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCg                                              <  4:207186/149‑1 (MQ=255)
                                                                                                  gcaTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCgg                                             <  3:1340851/149‑1 (MQ=255)
                                                                                                  gcaTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATAAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       >  2:1533796/1‑107 (MQ=255)
                                                                                                    aTCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCtggttggt                                                                                                <  3:598542/96‑1 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGaa                                                                                           <  3:570357/100‑1 (MQ=255)
                                                                                                     tCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCa                                          >  2:185749/1‑149 (MQ=255)
                                                                                                       aGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                                      <  3:207278/71‑1 (MQ=255)
                                                                                                        gAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAATGAGTCATTATGGCTggttggtt                                                                                               >  3:1290580/1‑93 (MQ=255)
                                                                                                        gAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                       <  2:378390/149‑1 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                                   >  4:1676024/1‑149 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTg                                    <  4:1148130/148‑1 (MQ=255)
                                                                                                            aTCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTg                                    >  3:1148130/1‑148 (MQ=255)
                                                                                                              cGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                                 <  4:1295379/149‑1 (MQ=255)
                                                                                                               gCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTc                                                                <  1:988215/117‑1 (MQ=255)
                                                                                                                cTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACt                               >  2:879244/1‑149 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       >  3:771170/1‑92 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       >  3:692875/1‑92 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTa                              >  1:947267/1‑149 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGACTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTa                              >  1:748366/1‑149 (MQ=255)
                                                                                                                 tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGCAGTCATTATGGCTGGTTGGTTTGAACTCa                                                                                       <  4:918139/92‑1 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                                 <  4:716465/144‑1 (MQ=255)
                                                                                                                   aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                            >  2:1794701/1‑149 (MQ=255)
                                                                                                                    tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTACAAGCGGGCAATg                                       >  3:1555082/1‑137 (MQ=255)
                                                                                                                     aCTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCt                          >  1:1503471/1‑149 (MQ=255)
                                                                                                                      cTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTc                         >  4:1569054/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGt                                                                                                                 >  1:877510/1‑60 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTga                                   <  3:1800759/138‑1 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                        >  3:461109/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                        >  1:1414114/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                        >  1:1481007/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                        >  3:1399807/1‑149 (MQ=255)
                                                                                                                       tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                        <  3:1505967/149‑1 (MQ=255)
                                                                                                                          aaTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCa                     >  1:1139120/1‑149 (MQ=255)
                                                                                                                           aTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCggg                                            >  4:1384548/1‑125 (MQ=255)
                                                                                                                           aTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGt                                     <  4:1411055/132‑1 (MQ=255)
                                                                                                                           aTCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAg                    >  3:1238246/1‑149 (MQ=255)
                                                                                                                               gaaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGt                                                                            >  4:1218877/3‑89 (MQ=38)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAg                                                                   >  4:576442/1‑98 (MQ=255)
                                                                                                                               gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAg                >  3:1633104/1‑149 (MQ=255)
                                                                                                                                aaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                                 >  1:472587/2‑131 (MQ=255)
                                                                                                                                aaaaCAGCATCTAAAGGTGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTgaga                                 <  2:472587/130‑1 (MQ=255)
                                                                                                                                 aaaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                            >  4:1824106/1‑135 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTtcaatcaa                                                                                                                      <  4:644022/43‑1 (MQ=255)
                                                                                                                                   aCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCttt            <  4:812551/149‑1 (MQ=255)
                                                                                                                                     aGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATg                                       <  2:984771/120‑1 (MQ=255)
                                                                                                                                      gCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTtata         <  2:1139120/149‑1 (MQ=255)
                                                                                                                                       cACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCa                                                                    <  2:1337002/89‑1 (MQ=255)
                                                                                                                                       cACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCa                                                                    >  1:1337002/1‑89 (MQ=255)
                                                                                                                                        aCCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTAt                                                                                                           <  3:398650/49‑1 (MQ=255)
                                                                                                                                          cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                        >  1:628735/1‑98 (MQ=255)
                                                                                                                                          cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTtgt                                                        <  2:628735/98‑1 (MQ=255)
                                                                                                                                             tAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCaaa  <  4:338183/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GACATTATTTTGTTAGCCGGAGATGGCGTTCAGACCAGATCCGGCAACATTATCCCACGCATGGTCAGCAAACTGACATTATCTCTCCGCCCTGGCAGGCATCAGAAAATCGCTCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAA  >  NC_000913/2165006‑2165295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: