Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,180,043 C→T 100% T408M (ACG→ATG)  yegT → nucleoside transporter, low affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,180,0430CT98.1% 766.5 / 1.8 207T408M (ACG→ATG) yegTnucleoside transporter, low affinity
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (102/100);  total (104/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 207 total aligned reads displayed.

GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAA  >  NC_000913/2179897‑2180190
                                                                                                                                                  |                                                                                                                                                   
     tgGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATc                                                                                                                                              >  4:887700/1‑149 (MQ=255)
     tgGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATc                                                                                                                                              >  3:477600/1‑149 (MQ=255)
         aaaGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAgg                                                                                                                                          >  2:1278249/1‑149 (MQ=255)
         aaaGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAgg                                                                                                                                          >  2:968875/1‑149 (MQ=255)
            gATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGt                                                                                                                                         <  1:287328/147‑1 (MQ=255)
              tGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCgat                                                                                                                                     >  3:669822/1‑149 (MQ=255)
                ttCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCgatga                                                                                                                                   >  4:1659914/1‑149 (MQ=255)
                ttCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGCGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCgatga                                                                                                                                   >  1:971205/1‑149 (MQ=255)
                  cGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATc                                                                                                                                 <  1:1047382/149‑1 (MQ=255)
                   gCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcg                                                                                                                                >  2:533576/1‑149 (MQ=255)
                   gCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcg                                                                                                                                >  4:607301/1‑149 (MQ=255)
                     ttATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcgcg                                                                                                                              >  3:869845/1‑149 (MQ=255)
                       aTCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGat                                                                                                                            >  3:1828577/1‑149 (MQ=255)
                       aTCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGat                                                                                                                            <  4:1359644/149‑1 (MQ=255)
                       aTCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGat                                                                                                                            >  2:1386552/1‑149 (MQ=255)
                              aCCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGt                                                                                                                     >  2:453412/1‑149 (MQ=255)
                              aCCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGt                                                                                                                     <  4:935753/149‑1 (MQ=255)
                               ccGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGtt                                                                                                                    <  1:453412/149‑1 (MQ=255)
                               ccGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGtt                                                                                                                    <  1:533576/149‑1 (MQ=255)
                                 ggTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa                                                                                                                  <  1:958406/149‑1 (MQ=255)
                                  gTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGac                                                                                                                 <  4:869188/149‑1 (MQ=255)
                                    aaaCGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGacac                                                                                                               >  2:1800846/1‑149 (MQ=255)
                                      aCGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACaa                                                                                                             >  4:31276/1‑149 (MQ=255)
                                      aCGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACaa                                                                                                             <  2:1135310/149‑1 (MQ=255)
                                      aCGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACaa                                                                                                             <  3:1542984/149‑1 (MQ=255)
                                      aCGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTCTACCGGGAATCCGAATACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACaa                                                                                                             <  3:717780/149‑1 (MQ=255)
                                        ggACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAgg                                                                                                           <  1:1356738/149‑1 (MQ=255)
                                         gactgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa                                                                                                                  >  3:1363920/1‑141 (MQ=255)
                                         gactgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAggg                                                                                                          <  3:327841/149‑1 (MQ=255)
                                         gactgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCCTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa                                                                                                                  <  4:1363920/141‑1 (MQ=255)
                                            tgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa                                                                                                                  >  1:1574222/1‑138 (MQ=255)
                                            tgactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGaa                                                                                                       >  4:132441/1‑149 (MQ=255)
                                             gactTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAg                                                                                                      <  4:222825/149‑1 (MQ=255)
                                              actTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGt                                                                                                     >  2:635600/1‑149 (MQ=255)
                                                tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGc                                                                                                                                                  >  3:1673278/1‑102 (MQ=255)
                                                tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGc                                                                                                                                                  <  4:1673278/102‑1 (MQ=255)
                                                tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATcgc                                                                                                                               >  4:664170/1‑121 (MQ=255)
                                                tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTa                                                                                                   >  1:66468/1‑149 (MQ=255)
                                                tttCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTa                                                                                                   >  3:1090232/1‑149 (MQ=255)
                                                 ttCAACTGGTACGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTaa                                                                                                  >  3:116468/1‑149 (MQ=255)
                                                  tCAACTGGTCCGGGATGTGGTCTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTTTTTATTTTCTTCCGGAAATCCGAAAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTaaa                                                                                                 <  3:1845507/149‑1 (MQ=255)
                                                  tCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTaaa                                                                                                 <  4:559126/149‑1 (MQ=255)
                                                    aaCTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATg                                                                                               >  3:642692/1‑149 (MQ=255)
                                                    aaCTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATg                                                                                               >  2:1476164/1‑149 (MQ=255)
                                                     aCTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATc                                                                                                                                 >  2:1657215/1‑114 (MQ=255)
                                                          tCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACa                                                                                         >  2:1761148/1‑149 (MQ=255)
                                                             gggATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGaa                                                                                      <  1:1386552/149‑1 (MQ=255)
                                                             gggATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGaa                                                                                      <  3:1659914/149‑1 (MQ=255)
                                                             gggATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGaa                                                                                      <  4:670789/149‑1 (MQ=255)
                                                               gATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGa                                                                                              >  3:290110/1‑139 (MQ=255)
                                                                   tgGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGt                                                                                   >  1:1185027/1‑146 (MQ=255)
                                                                     gACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCga                                                                                                                                      >  1:907005/1‑93 (MQ=255)
                                                                     gACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATtct                                                                              >  1:1853778/1‑149 (MQ=255)
                                                                      aCTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATtctc                                                                             <  1:1761148/149‑1 (MQ=255)
                                                                       cTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCg                                                                            <  2:1045736/149‑1 (MQ=255)
                                                                        tttCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCgg                                                                           >  4:484163/1‑149 (MQ=255)
                                                                         ttCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGt                                                                          >  3:566704/1‑149 (MQ=255)
                                                                                 ggTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTa                                                                  <  3:1280046/149‑1 (MQ=255)
                                                                                    gatgatTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATgg                                                               >  4:1075122/1‑149 (MQ=255)
                                                                                        atTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGaa                                                                                      <  3:1450591/122‑1 (MQ=255)
                                                                                        atTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGaa                                                                                      >  4:1450591/1‑122 (MQ=255)
                                                                                        atTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAg                                                           >  4:1026806/1‑149 (MQ=255)
                                                                                            ccATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGt                                                                                                                     <  1:1268176/87‑1 (MQ=255)
                                                                                             cATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaaa                                                                                            >  4:77482/1‑111 (MQ=255)
                                                                                             cATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGaaa                                                                                            <  3:77482/111‑1 (MQ=255)
                                                                                               ttATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAATGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAg                                                    >  1:1816127/1‑149 (MQ=255)
                                                                                               ttATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAg                                                    >  2:415204/1‑149 (MQ=255)
                                                                                                tATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAgg                                                   <  1:635600/149‑1 (MQ=255)
                                                                                                 aTCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAggg                                                  <  1:415204/149‑1 (MQ=255)
                                                                                                     ccGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAg                                                                                                            <  2:1584275/87‑1 (MQ=255)
                                                                                                        tGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCTAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTa                                                                  <  4:1426286/126‑1 (MQ=255)
                                                                                                        tGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCTAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTa                                                                  >  3:1426286/1‑126 (MQ=255)
                                                                                                           tGTTCATGATTTTTTTCCGCGAATCCGACAGCGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATgg                                        <  1:1450149/149‑1 (MQ=255)
                                                                                                           tGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGa                                                                                              <  2:1102497/95‑1 (MQ=255)
                                                                                                           tGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGa                                                                                              >  1:1102497/1‑95 (MQ=255)
                                                                                                             ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGtt                                                                                                                    >  2:1226806/1‑71 (MQ=255)
                                                                                                             ttCATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAggg                                                  >  3:1354841/1‑137 (MQ=255)
                                                                                                             ttCATGATTTTATTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATgggg                                      >  1:1619658/1‑149 (MQ=255)
                                                                                                               cATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGAt                                    >  4:112522/1‑149 (MQ=255)
                                                                                                               cATGATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGAt                                    <  4:1828577/149‑1 (MQ=255)
                                                                                                                  gATTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATggg                                                              >  2:340832/1‑120 (MQ=255)
                                                                                                                   aTTTTTTTCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGa                                                                                                                  >  2:1828761/1‑67 (MQ=255)
                                                                                                                     ttttttCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTc                              >  1:1022695/1‑149 (MQ=255)
                                                                                                                         ttCCGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATg                                         >  2:912997/1‑134 (MQ=255)
                                                                                                                           ccGCGAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCt                        >  4:1626051/1‑149 (MQ=255)
                                                                                                                             gcgAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCttt                      >  1:756489/1‑149 (MQ=255)
                                                                                                                               gAATCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTgg                    <  3:535318/149‑1 (MQ=255)
                                                                                                                                aaTCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGc                                                         >  2:773716/1‑111 (MQ=255)
                                                                                                                                aaTCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGc                   >  1:1881394/1‑149 (MQ=255)
                                                                                                                                aaTCCGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGc                   <  3:1026806/149‑1 (MQ=255)
                                                                                                                                   ccGACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCtt                                                                    <  3:163384/97‑1 (MQ=255)
                                                                                                                                     gACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGc              >  4:473149/1‑149 (MQ=255)
                                                                                                                                     gACAACGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGc              >  4:1222808/1‑149 (MQ=255)
                                                                                                                                        aaCGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGc           >  1:79680/1‑149 (MQ=255)
                                                                                                                                         aCGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGcc          <  3:90324/149‑1 (MQ=255)
                                                                                                                                         aCGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGcc          >  4:1387018/1‑149 (MQ=255)
                                                                                                                                          cGAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCg                            >  4:148127/1‑130 (MQ=255)
                                                                                                                                          cGAAATTATGGCTAACAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCg                            <  3:148127/130‑1 (MQ=255)
                                                                                                                                           gAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCAc                >  3:805977/1‑141 (MQ=255)
                                                                                                                                           gAAATTATGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCAc                <  4:805977/141‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATGATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGGATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTCCGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACACAAGGGGAAGTTAAATGAAAACAGAACGTATTCTCGGTGCTCTTTATGGGCAGGCGTTAGGGGATGCGATGGGGATGCCCTCCGAGCTTTGGCCACGCAGCCGCGTTAAA  >  NC_000913/2179897‑2180190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: