Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,419 C→T 5.4% A114A (GCG→GCA bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,4190CT 5.4% 317.2 / 6.3 93A114A (GCG→GCAbcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (42/46);  new base T (2/3);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 104 total aligned reads displayed.

GCCAGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCC  >  NC_000913/2279271‑2279565
                                                                                                                                                    |                                                                                                                                                  
gCCAGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgcc                                                                                                                                                    >  4:1548888/1‑149 (MQ=255)
  cAGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgcc                                                                                                                                                    <  1:111111/147‑1 (MQ=255)
  cAGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgcc                                                                                                                                                    >  2:111111/1‑147 (MQ=255)
   aGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgca                                                                                                                                                 >  3:1066928/1‑149 (MQ=255)
     cTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagc                                                                                                                                               <  1:1273458/149‑1 (MQ=255)
       cAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagccg                                                                                                                                             >  1:1590483/1‑149 (MQ=255)
          ccACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGccgcagccgcag                                                                                                                                          >  1:1872144/1‑149 (MQ=255)
            acacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCgcagccgcagcc                                                                                                                                        >  1:872737/1‑149 (MQ=255)
             cacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagcca                                                                                                                                       <  4:1819155/149‑1 (MQ=255)
                 aGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagcgt                                                                                                                                   <  3:414933/149‑3 (MQ=255)
                  gCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCg                                                                                                                                  <  1:239814/149‑1 (MQ=255)
                      ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                              >  4:563726/1‑149 (MQ=255)
                      ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                              >  2:1368613/1‑149 (MQ=255)
                       cAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                             >  2:563393/1‑149 (MQ=255)
                           cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                         <  3:1548888/149‑1 (MQ=255)
                           cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                         <  4:1069079/149‑1 (MQ=255)
                           cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                         <  3:422685/149‑1 (MQ=255)
                           cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                         <  3:563726/149‑1 (MQ=255)
                            gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                        <  3:1778851/149‑1 (MQ=255)
                            gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                        <  3:418506/149‑1 (MQ=255)
                             ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                     <  2:224708/135‑1 (MQ=255)
                             ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                     <  2:1540932/135‑1 (MQ=255)
                             ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                     >  1:1540932/1‑135 (MQ=255)
                             ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                     >  1:224708/1‑135 (MQ=255)
                             ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                       >  4:1807729/1‑149 (MQ=255)
                               aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                     <  1:299893/149‑1 (MQ=255)
                               aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                     >  3:507728/1‑149 (MQ=255)
                                aCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCAt                                                                                                                    >  3:265086/1‑149 (MQ=255)
                                  tATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATca                                                                                                                  >  4:1067465/1‑149 (MQ=255)
                                       gTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATc                                                                                                             >  1:291861/1‑149 (MQ=255)
                                          ccATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGc                                                                                                          <  4:265086/149‑1 (MQ=255)
                                           cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTg                                                                                                                                <  3:934911/126‑1 (MQ=255)
                                           cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCt                                                                                                         <  3:1807729/149‑1 (MQ=255)
                                            aTCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                        >  2:1020511/1‑149 (MQ=255)
                                              cAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGAt                                                                                                      <  4:1066928/149‑1 (MQ=255)
                                                  ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                  <  2:1872144/149‑1 (MQ=255)
                                                  ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                  <  1:1368613/149‑1 (MQ=255)
                                                         tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           <  3:1024548/149‑1 (MQ=255)
                                                         tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           <  2:291861/149‑1 (MQ=255)
                                                           gTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGc                                                                                         <  1:1020511/149‑1 (MQ=255)
                                                            ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                     <  3:1667121/104‑1 (MQ=255)
                                                            ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                     >  4:1667121/1‑104 (MQ=255)
                                                            ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGcc                                                                                        <  2:1590483/149‑1 (MQ=255)
                                                               tCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                     <  3:1125329/149‑1 (MQ=255)
                                                                 aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                   >  3:220488/1‑149 (MQ=255)
                                                                 aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                   >  1:1447878/1‑149 (MQ=255)
                                                                    aGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGcaca                                                                                <  3:1067465/149‑1 (MQ=255)
                                                                     gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                   >  3:210530/1‑97 (MQ=255)
                                                                     gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                   <  4:210530/97‑1 (MQ=255)
                                                                     gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               <  2:872737/149‑1 (MQ=255)
                                                                      cATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATc                                                                                                     >  3:1306440/1‑126 (MQ=255)
                                                                      cATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATc                                                                                                     <  4:1306440/126‑1 (MQ=255)
                                                                          aCAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCACGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCAc                                                                          <  1:563393/149‑1 (MQ=255)
                                                                            aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                     <  2:1821916/136‑1 (MQ=255)
                                                                            aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                     >  1:1821916/1‑136 (MQ=255)
                                                                             aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                       >  4:1247123/1‑149 (MQ=255)
                                                                             aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                       <  4:507728/149‑1 (MQ=255)
                                                                              aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                  <  2:1625513/121‑1 (MQ=255)
                                                                              aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                  >  1:1625513/1‑121 (MQ=255)
                                                                              aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                 >  1:1287220/1‑122 (MQ=255)
                                                                              aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                 <  2:1287220/122‑1 (MQ=255)
                                                                                 aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               <  1:172432/137‑1 (MQ=255)
                                                                                 aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               >  2:172432/1‑137 (MQ=255)
                                                                                      atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               <  3:1598077/132‑1 (MQ=255)
                                                                                      atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               >  4:1598077/1‑132 (MQ=255)
                                                                                       tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                              >  1:1009139/1‑148 (MQ=255)
                                                                                       tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                              <  2:1009139/148‑1 (MQ=255)
                                                                                       tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCa                                                             >  4:1432503/1‑149 (MQ=255)
                                                                                              gaACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                      <  3:1432503/149‑1 (MQ=255)
                                                                                                 ctctTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                             <  2:611122/75‑1 (MQ=255)
                                                                                                 ctctTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                             >  1:611122/1‑75 (MQ=255)
                                                                                                  tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           <  2:1587878/108‑1 (MQ=255)
                                                                                                  tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           <  2:1736159/108‑1 (MQ=255)
                                                                                                  tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           >  1:1736159/1‑108 (MQ=255)
                                                                                                  tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           >  1:1587878/1‑108 (MQ=255)
                                                                                                    ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           <  2:1165317/106‑1 (MQ=255)
                                                                                                    ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                           >  1:1165317/1‑106 (MQ=255)
                                                                                                    ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                  <  2:200642/131‑1 (MQ=255)
                                                                                                    ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                  >  1:200642/1‑131 (MQ=255)
                                                                                                      cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                >  1:921119/1‑99 (MQ=255)
                                                                                                      cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                <  2:921119/99‑1 (MQ=255)
                                                                                                       tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                      <  3:1325187/140‑1 (MQ=255)
                                                                                                       tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                      >  4:1325187/1‑140 (MQ=255)
                                                                                                             gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               >  2:213611/1‑109 (MQ=255)
                                                                                                             gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                               <  1:213611/109‑1 (MQ=255)
                                                                                                                 atatCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGcaccac                                   <  3:1247123/149‑1 (MQ=255)
                                                                                                                   atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCg                                 >  1:1305435/1‑149 (MQ=255)
                                                                                                                             aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGa                                          >  2:1677284/1‑130 (MQ=255)
                                                                                                                             aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGa                                          <  1:1677284/130‑1 (MQ=255)
                                                                                                                                        gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                            <  4:1743659/133‑1 (MQ=255)
                                                                                                                                        gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                            >  3:1743659/1‑133 (MQ=255)
                                                                                                                                        gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg            >  4:1625476/1‑149 (MQ=255)
                                                                                                                                        gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg            >  3:113954/1‑149 (MQ=255)
                                                                                                                                          ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                           >  2:1102430/1‑100 (MQ=255)
                                                                                                                                          ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                           <  1:1102430/100‑1 (MQ=255)
                                                                                                                                            aCGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCAt        >  4:1384810/1‑149 (MQ=255)
                                                                                                                                                  gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                       >  4:503647/1‑112 (MQ=255)
                                                                                                                                                  gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                       <  3:503647/112‑1 (MQ=255)
                                                                                                                                                  gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc  <  4:220488/149‑1 (MQ=255)
                                                                                                                                                  gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc  <  2:1447878/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCAGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCC  >  NC_000913/2279271‑2279565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: