Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,422 A→G 5.4% A113A (GCT→GCC bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,4220AG 5.4% 318.8 / 6.1 92A113A (GCT→GCCbcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (41/46);  new base G (2/3);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 105 total aligned reads displayed.

AGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGA  >  NC_000913/2279274‑2279570
                                                                                                                                                    |                                                                                                                                                    
aGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgca                                                                                                                                                      >  3:1066928/1‑149 (MQ=255)
  cTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagc                                                                                                                                                    <  1:1273458/149‑1 (MQ=255)
    cAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagccg                                                                                                                                                  >  1:1590483/1‑149 (MQ=255)
       ccACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGccgcagccgcag                                                                                                                                               >  1:1872144/1‑149 (MQ=255)
         acacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCgcagccgcagcc                                                                                                                                             >  1:872737/1‑149 (MQ=255)
          cacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagcca                                                                                                                                            <  4:1819155/149‑1 (MQ=255)
              aGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagcgt                                                                                                                                        <  3:414933/149‑3 (MQ=255)
               gCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCg                                                                                                                                       <  1:239814/149‑1 (MQ=255)
                   ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                   >  4:563726/1‑149 (MQ=255)
                   ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                   >  1:511533/1‑149 (MQ=255)
                   ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                   >  2:1368613/1‑149 (MQ=255)
                    cAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                                  >  2:563393/1‑149 (MQ=255)
                        cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                              <  3:1548888/149‑1 (MQ=255)
                        cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                              <  3:563726/149‑1 (MQ=255)
                        cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                              <  3:422685/149‑1 (MQ=255)
                        cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                              <  4:1069079/149‑1 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                             <  3:1778851/149‑1 (MQ=255)
                         gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                             <  3:418506/149‑1 (MQ=255)
                          ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                          <  2:224708/135‑1 (MQ=255)
                          ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                          <  2:1540932/135‑1 (MQ=255)
                          ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                          >  1:1540932/1‑135 (MQ=255)
                          ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                            >  4:1807729/1‑149 (MQ=255)
                            aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                          <  1:299893/149‑1 (MQ=255)
                            aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                          >  3:507728/1‑149 (MQ=255)
                             aCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCAt                                                                                                                         >  3:265086/1‑149 (MQ=255)
                               tATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATca                                                                                                                       >  4:1067465/1‑149 (MQ=255)
                                    gTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATc                                                                                                                  >  1:291861/1‑149 (MQ=255)
                                       ccATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGc                                                                                                               <  4:265086/149‑1 (MQ=255)
                                        cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTg                                                                                                                                     <  3:934911/126‑1 (MQ=255)
                                        cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCt                                                                                                              <  3:1807729/149‑1 (MQ=255)
                                         aTCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                             >  2:1020511/1‑149 (MQ=255)
                                               ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                       <  1:1368613/149‑1 (MQ=255)
                                               ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                       <  2:1872144/149‑1 (MQ=255)
                                                      tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                <  3:1024548/149‑1 (MQ=255)
                                                      tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                <  2:291861/149‑1 (MQ=255)
                                                        gTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGc                                                                                              <  1:1020511/149‑1 (MQ=255)
                                                         ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                          <  3:1667121/104‑1 (MQ=255)
                                                         ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                          >  4:1667121/1‑104 (MQ=255)
                                                         ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGcc                                                                                             <  2:1590483/149‑1 (MQ=255)
                                                            tCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                          <  3:1125329/149‑1 (MQ=255)
                                                              aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                        >  3:220488/1‑149 (MQ=255)
                                                              aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                        >  1:1447878/1‑149 (MQ=255)
                                                                 aGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGcaca                                                                                     <  3:1067465/149‑1 (MQ=255)
                                                                  gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                        >  3:210530/1‑97 (MQ=255)
                                                                  gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                        <  4:210530/97‑1 (MQ=255)
                                                                  gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    <  2:872737/149‑1 (MQ=255)
                                                                   cATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATc                                                                                                          <  4:1306440/126‑1 (MQ=255)
                                                                       aCAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCACGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCAc                                                                               <  1:563393/149‑1 (MQ=255)
                                                                         aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                          <  2:1821916/136‑1 (MQ=255)
                                                                         aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                          >  1:1821916/1‑136 (MQ=255)
                                                                          aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                            >  4:1247123/1‑149 (MQ=255)
                                                                          aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                            <  4:507728/149‑1 (MQ=255)
                                                                           aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                       <  2:1625513/121‑1 (MQ=255)
                                                                           aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                       >  1:1625513/1‑121 (MQ=255)
                                                                           aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                      <  2:1287220/122‑1 (MQ=255)
                                                                           aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                      >  1:1287220/1‑122 (MQ=255)
                                                                              aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    <  1:172432/137‑1 (MQ=255)
                                                                              aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    >  2:172432/1‑137 (MQ=255)
                                                                                   atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    >  4:1598077/1‑132 (MQ=255)
                                                                                   atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    <  3:1598077/132‑1 (MQ=255)
                                                                                    tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                                   >  1:1009139/1‑148 (MQ=255)
                                                                                    tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                                   <  2:1009139/148‑1 (MQ=255)
                                                                                    tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCa                                                                  >  4:1432503/1‑149 (MQ=255)
                                                                                           gaACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                           <  3:1432503/149‑1 (MQ=255)
                                                                                              ctctTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                                  <  2:611122/75‑1 (MQ=255)
                                                                                              ctctTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                                  >  1:611122/1‑75 (MQ=255)
                                                                                               tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                >  1:1736159/1‑108 (MQ=255)
                                                                                               tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                >  1:1587878/1‑108 (MQ=255)
                                                                                               tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                <  2:1587878/108‑1 (MQ=255)
                                                                                               tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                <  2:1736159/108‑1 (MQ=255)
                                                                                                 ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                <  2:1165317/106‑1 (MQ=255)
                                                                                                 ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                >  1:1165317/1‑106 (MQ=255)
                                                                                                 ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                       >  1:200642/1‑131 (MQ=255)
                                                                                                 ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                       <  2:200642/131‑1 (MQ=255)
                                                                                                   cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                     <  2:921119/99‑1 (MQ=255)
                                                                                                   cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                     >  1:921119/1‑99 (MQ=255)
                                                                                                    tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                           <  3:1325187/140‑1 (MQ=255)
                                                                                                    tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                           >  4:1325187/1‑140 (MQ=255)
                                                                                                          gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    >  2:213611/1‑109 (MQ=255)
                                                                                                          gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                    <  1:213611/109‑1 (MQ=255)
                                                                                                              atatCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGcaccac                                        <  3:1247123/149‑1 (MQ=255)
                                                                                                                atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCg                                      >  1:1305435/1‑149 (MQ=255)
                                                                                                                          aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGa                                               <  1:1677284/130‑1 (MQ=255)
                                                                                                                                ttAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCt                      >  2:542351/1‑149 (MQ=255)
                                                                                                                                     gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                                 <  4:1743659/133‑1 (MQ=255)
                                                                                                                                     gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                                 >  3:1743659/1‑133 (MQ=255)
                                                                                                                                     gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg                 >  3:113954/1‑149 (MQ=255)
                                                                                                                                     gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg                 >  4:1625476/1‑149 (MQ=255)
                                                                                                                                      aCCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGc                >  3:423909/1‑149 (MQ=255)
                                                                                                                                       ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                                <  1:1102430/100‑1 (MQ=255)
                                                                                                                                       ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                                >  2:1102430/1‑100 (MQ=255)
                                                                                                                                         aCGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCAt             >  4:1384810/1‑149 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                            <  3:503647/112‑1 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                            >  4:503647/1‑112 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc       <  4:220488/149‑1 (MQ=255)
                                                                                                                                               gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc       <  2:1447878/149‑1 (MQ=255)
                                                                                                                                                  gcagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACACCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTa    <  3:1625476/149‑1 (MQ=255)
                                                                                                                                                   cagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAacac                                                             >  4:1280784/1‑91 (MQ=255)
                                                                                                                                                   cagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAacac                                                             <  3:1280784/91‑1 (MQ=255)
                                                                                                                                                    agccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGa  <  2:1305435/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCTCAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGA  >  NC_000913/2279274‑2279570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: