Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,279,425 C→T 5.4% A112A (GCG→GCA)  bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,279,4250CT 5.4% 306.6 / 6.5 92A112A (GCG→GCA) bcrbicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (41/46);  new base T (2/3);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-02

Only 100 of 104 total aligned reads displayed.

CAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGAT  >  NC_000913/2279278‑2279571
                                                                                                                                                   |                                                                                                                                                  
cAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGgccgcagccg                                                                                                                                                   >  1:1590483/1‑149 (MQ=255)
   ccACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGccgcagccgcag                                                                                                                                                >  1:1872144/1‑149 (MQ=255)
     acacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCgcagccgcagcc                                                                                                                                              >  1:872737/1‑149 (MQ=255)
      cacCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagcca                                                                                                                                             <  4:1819155/149‑1 (MQ=255)
          aGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagcgt                                                                                                                                         <  3:414933/149‑3 (MQ=255)
           gCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCg                                                                                                                                        <  1:239814/149‑1 (MQ=255)
               ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                    >  4:563726/1‑149 (MQ=255)
               ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                    >  2:1368613/1‑149 (MQ=255)
               ccAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGga                                                                                                                                    >  1:511533/1‑149 (MQ=255)
                    cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                               <  3:563726/149‑1 (MQ=255)
                    cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                               <  3:1548888/149‑1 (MQ=255)
                    cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                               <  3:422685/149‑1 (MQ=255)
                    cgccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGaaa                                                                                                                               <  4:1069079/149‑1 (MQ=255)
                     gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                              <  3:418506/149‑1 (MQ=255)
                     gccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAAc                                                                                                                              <  3:1778851/149‑1 (MQ=255)
                      ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                           >  1:1540932/1‑135 (MQ=255)
                      ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                           <  2:1540932/135‑1 (MQ=255)
                      ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                           <  2:224708/135‑1 (MQ=255)
                      ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCTGCGGCTGcggccagc                                                                                                                                           >  1:224708/1‑135 (MQ=255)
                      ccAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACg                                                                                                                             >  4:1807729/1‑149 (MQ=255)
                        aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                           <  1:299893/149‑1 (MQ=255)
                        aaCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCa                                                                                                                           >  3:507728/1‑149 (MQ=255)
                         aCTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCAt                                                                                                                          >  3:265086/1‑149 (MQ=255)
                           tATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATca                                                                                                                        >  4:1067465/1‑149 (MQ=255)
                                gTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATc                                                                                                                   >  1:291861/1‑149 (MQ=255)
                                   ccATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGc                                                                                                                <  4:265086/149‑1 (MQ=255)
                                    cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTg                                                                                                                                      <  3:934911/126‑1 (MQ=255)
                                    cATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCt                                                                                                               <  3:1807729/149‑1 (MQ=255)
                                     aTCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTg                                                                                                              >  2:1020511/1‑149 (MQ=255)
                                       cAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGAt                                                                                                            <  4:1066928/149‑1 (MQ=255)
                                           ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                        <  1:1368613/149‑1 (MQ=255)
                                           ggTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                        <  2:1872144/149‑1 (MQ=255)
                                                  tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 <  3:1024548/149‑1 (MQ=255)
                                                  tGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 <  2:291861/149‑1 (MQ=255)
                                                    gTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGc                                                                                               <  1:1020511/149‑1 (MQ=255)
                                                     ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                           <  3:1667121/104‑1 (MQ=255)
                                                     ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGcagccagc                                                                                                                                           >  4:1667121/1‑104 (MQ=255)
                                                     ttGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGcc                                                                                              <  2:1590483/149‑1 (MQ=255)
                                                        tCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                           <  3:1125329/149‑1 (MQ=255)
                                                          aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                         >  3:220488/1‑149 (MQ=255)
                                                          aCCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGc                                                                                         >  1:1447878/1‑149 (MQ=255)
                                                             aGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGcaca                                                                                      <  3:1067465/149‑1 (MQ=255)
                                                              gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                         >  3:210530/1‑97 (MQ=255)
                                                              gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGccc                                                                                                                                         <  4:210530/97‑1 (MQ=255)
                                                              gCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     <  2:872737/149‑1 (MQ=255)
                                                               cATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATc                                                                                                           >  3:1306440/1‑126 (MQ=255)
                                                               cATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATc                                                                                                           <  4:1306440/126‑1 (MQ=255)
                                                                   aCAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCACGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCAc                                                                                <  1:563393/149‑1 (MQ=255)
                                                                     aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                           >  1:1821916/1‑136 (MQ=255)
                                                                     aaaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAAc                                                                                           <  2:1821916/136‑1 (MQ=255)
                                                                      aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                             >  4:1247123/1‑149 (MQ=255)
                                                                      aaCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACcgc                                                                             <  4:507728/149‑1 (MQ=255)
                                                                       aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                        <  2:1625513/121‑1 (MQ=255)
                                                                       aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGAt                                                                                                        >  1:1625513/1‑121 (MQ=255)
                                                                       aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                       <  2:1287220/122‑1 (MQ=255)
                                                                       aCGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATg                                                                                                       >  1:1287220/1‑122 (MQ=255)
                                                                          aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     <  1:172432/137‑1 (MQ=255)
                                                                          aCATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     >  2:172432/1‑137 (MQ=255)
                                                                               atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     >  4:1598077/1‑132 (MQ=255)
                                                                               atcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     <  3:1598077/132‑1 (MQ=255)
                                                                                tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                                    >  1:1009139/1‑148 (MQ=255)
                                                                                tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCggcggcggc                                                                    <  2:1009139/148‑1 (MQ=255)
                                                                                tcCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCa                                                                   >  4:1432503/1‑149 (MQ=255)
                                                                                       gaACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                            <  3:1432503/149‑1 (MQ=255)
                                                                                          ctctTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGgaa                                                                                                                                   <  2:611122/75‑1 (MQ=255)
                                                                                           tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 <  2:1587878/108‑1 (MQ=255)
                                                                                           tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 >  1:1736159/1‑108 (MQ=255)
                                                                                           tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 >  1:1587878/1‑108 (MQ=255)
                                                                                           tcttctTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 <  2:1736159/108‑1 (MQ=255)
                                                                                             ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 <  2:1165317/106‑1 (MQ=255)
                                                                                             ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGttt                                                                                                 >  1:1165317/1‑106 (MQ=255)
                                                                                             ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                        >  1:200642/1‑131 (MQ=255)
                                                                                             ttcttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCgcggcgg                                                                        <  2:200642/131‑1 (MQ=255)
                                                                                               cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                      >  1:921119/1‑99 (MQ=255)
                                                                                               cttTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATgg                                                                                                      <  2:921119/99‑1 (MQ=255)
                                                                                                tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                            >  4:1325187/1‑140 (MQ=255)
                                                                                                tttCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCa                                                            <  3:1325187/140‑1 (MQ=255)
                                                                                                      gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     <  1:213611/109‑1 (MQ=255)
                                                                                                      gTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCacac                                                                                     >  2:213611/1‑109 (MQ=255)
                                                                                                          atatCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGcaccac                                         <  3:1247123/149‑1 (MQ=255)
                                                                                                            atCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCg                                       >  1:1305435/1‑149 (MQ=255)
                                                                                                                      aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGa                                                <  1:1677284/130‑1 (MQ=255)
                                                                                                                      aGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGa                                                >  2:1677284/1‑130 (MQ=255)
                                                                                                                            ttAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCt                       >  2:542351/1‑149 (MQ=255)
                                                                                                                                 gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                                  <  4:1743659/133‑1 (MQ=255)
                                                                                                                                 gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTa                                  >  3:1743659/1‑133 (MQ=255)
                                                                                                                                 gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg                  >  4:1625476/1‑149 (MQ=255)
                                                                                                                                 gACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTg                  >  3:113954/1‑149 (MQ=255)
                                                                                                                                   ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                                 >  2:1102430/1‑100 (MQ=255)
                                                                                                                                   ccACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCaaa                                                                 <  1:1102430/100‑1 (MQ=255)
                                                                                                                                     aCGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCAt              >  4:1384810/1‑149 (MQ=255)
                                                                                                                                           gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                             <  3:503647/112‑1 (MQ=255)
                                                                                                                                           gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGca                                             >  4:503647/1‑112 (MQ=255)
                                                                                                                                           gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc        <  4:220488/149‑1 (MQ=255)
                                                                                                                                           gccgcagccgcaGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGccc        <  2:1447878/149‑1 (MQ=255)
                                                                                                                                              gcagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACACCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTa     <  3:1625476/149‑1 (MQ=255)
                                                                                                                                               cagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAacac                                                              >  4:1280784/1‑91 (MQ=255)
                                                                                                                                               cagccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAacac                                                              <  3:1280784/91‑1 (MQ=255)
                                                                                                                                                agccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGa   <  2:1305435/149‑1 (MQ=255)
                                                                                                                                                 gccgcagccAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGAt  >  2:1776912/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCCACACCAGCACCCAGCCGCCAACTATCGGTGCCATCAGCGGTGCAATGGTTGTCACCAGCATGACAAACGACATCATCCGCGAGAACTCTTCTTTCGGGTAAATATCGCGCATCAGGGCGTTAATGACCACGCTGGCCGCAGCCGCAGCCAGCCCGTGGAAGAAACGCATCACAATCAGCTGATCGATGGTGTTTGCCAACGCACACGCCACCGCGGCGGCGGCAAACACCAGCGTACCGCCGAGCACCACCGGCTTACGCCCGAAGCTGTCTGCCATCGGCCCGTAGAT  >  NC_000913/2279278‑2279571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑