Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,340,379 T→A 10.1% intergenic (+90/+38) ubiG → / ← yfaL bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,340,3790TA10.1% 260.0 / 16.0 79intergenic (+90/+38)ubiG/yfaLbifunctional 3‑demethylubiquinone‑9 3‑methyltransferase/ 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin
Reads supporting (aligned to +/- strand):  ref base T (38/33);  new base A (3/5);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCG  >  NC_000913/2340231‑2340526
                                                                                                                                                    |                                                                                                                                                   
cTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGtt                                                                                                                                                     <  3:640999/149‑1 (MQ=255)
 ttttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGttt                                                                                                                                                    >  2:1103057/1‑149 (MQ=255)
 ttttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCAACAAATGCTCCCCGTAGGCCGGATAAGGCGttt                                                                                                                                                    >  1:1453949/1‑149 (MQ=255)
      aaCTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                               >  2:305205/1‑149 (MQ=255)
          cGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                           <  2:126555/149‑5 (MQ=38)
          cGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                           <  3:64953/149‑5 (MQ=38)
           ggCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                          >  4:1850817/1‑149 (MQ=255)
             ccccGTCGTGGATGTGAACTTTATGCTGCACACGCAGAATAAGTCAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTTGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                        <  2:1453949/149‑1 (MQ=255)
              cccGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                       <  3:949244/149‑1 (MQ=38)
               ccGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                      <  1:10749/149‑1 (MQ=255)
                         tgtgAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                           >  1:1433269/1‑130 (MQ=38)
                         tgtgAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                           <  2:1433269/134‑5 (MQ=38)
                               cTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGa                                                                                                                      <  1:1103057/149‑1 (MQ=255)
                                tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                <  3:577350/122‑1 (MQ=255)
                                tataTGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                >  4:577350/1‑122 (MQ=255)
                                  tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                          >  2:1407101/1‑121 (MQ=38)
                                  tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                          <  1:1407101/126‑6 (MQ=38)
                                         cacaCGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTAc                                                                                                            <  3:1850817/149‑1 (MQ=255)
                                         cacaCGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGGGATTAc                                                                                                            >  1:631179/1‑149 (MQ=255)
                                            acGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATGCGGCGTAAAcgcct                                                                                                                                              >  4:535912/1‑111 (MQ=37)
                                             cGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACt                                                                                                                         <  3:218512/132‑1 (MQ=255)
                                             cGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACt                                                                                                                         >  4:218512/1‑132 (MQ=255)
                                              gCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAttt                                                                                                       >  4:515986/1‑149 (MQ=255)
                                                  aaTAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCAcc                                                                                                   <  2:631179/149‑1 (MQ=255)
                                                        tGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                         >  2:619164/1‑137 (MQ=255)
                                                        tGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                         <  1:619164/137‑1 (MQ=255)
                                                                aTGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACaaaaa                                                                                     >  3:47227/1‑149 (MQ=255)
                                                                  gTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTa                                                                                                                        >  3:1862601/1‑112 (MQ=255)
                                                                  gTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTa                                                                                                                        <  4:1862601/112‑1 (MQ=255)
                                                                  gTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAATcc                                                                                   <  3:515986/149‑1 (MQ=255)
                                                                    ttGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATc                                                                                             <  4:1707093/137‑1 (MQ=255)
                                                                    ttGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATc                                                                                             >  3:1707093/1‑137 (MQ=255)
                                                                             ccAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTgg                                                                        <  1:305205/149‑1 (MQ=255)
                                                                                    cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAttt                                                                                                       >  4:1666006/1‑111 (MQ=255)
                                                                                    cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAttt                                                                                                       <  3:1666006/111‑1 (MQ=255)
                                                                                            aCGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTc                                                         >  1:1457811/1‑149 (MQ=255)
                                                                                            aCGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTc                                                         >  1:1456991/1‑149 (MQ=255)
                                                                                              gCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTccc                                                       >  4:378456/1‑149 (MQ=255)
                                                                                                   ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc                                                                                  >  2:167840/1‑117 (MQ=255)
                                                                                                   ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAAccc                                                                                  <  1:167840/117‑1 (MQ=255)
                                                                                                   ccGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt                                                  >  1:1812827/1‑149 (MQ=255)
                                                                                                       gTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAAtcatca                                                            <  1:879270/135‑1 (MQ=255)
                                                                                                       gTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTCCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAAtcatca                                                            >  2:879270/1‑135 (MQ=255)
                                                                                                        tcgcaTCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCTTTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             <  2:1664582/145‑1 (MQ=255)
                                                                                                        tCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             <  3:378456/149‑1 (MQ=255)
                                                                                                        tCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             <  2:1456991/149‑1 (MQ=255)
                                                                                                        tCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             >  1:85495/1‑149 (MQ=255)
                                                                                                             tCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgtcac                                        >  1:1850257/1‑149 (MQ=255)
                                                                                                             tCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccacgccac                                        >  4:879288/1‑149 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAg                                                                                                                   <  3:686703/72‑1 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAg                                                                                                                   >  2:973611/1‑72 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAg                                                                                                                   <  1:973611/72‑1 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAg                                                                                                                   >  4:686703/1‑72 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCcacgccacgc                                      >  4:1285310/1‑149 (MQ=255)
                                                                                                               aGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCcacgccacgc                                      >  1:1074132/1‑149 (MQ=255)
                                                                                                                ggCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCt                                     >  2:1435371/1‑149 (MQ=255)
                                                                                                                  ccTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACa                                                                                                                    <  1:1861140/68‑1 (MQ=255)
                                                                                                                  ccTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACa                                                                                                                    >  2:1861140/1‑68 (MQ=255)
                                                                                                                    tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                         <  3:1695451/77‑1 (MQ=255)
                                                                                                                    tACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCAt                                                                                                         >  4:1695451/1‑77 (MQ=255)
                                                                                                                       aaaTGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGc                                                                               <  1:1810109/100‑1 (MQ=255)
                                                                                                                       aaaTGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGc                                                                               >  2:1810109/1‑100 (MQ=255)
                                                                                                                       aaaTGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             >  3:547812/1‑134 (MQ=255)
                                                                                                                       aaaTGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                             <  4:547812/134‑1 (MQ=255)
                                                                                                                          tGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAgg                           <  2:85495/149‑1 (MQ=255)
                                                                                                                           gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCt                                                                          <  3:1703190/101‑1 (MQ=255)
                                                                                                                           gCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCt                                                                          >  4:1703190/1‑101 (MQ=255)
                                                                                                                             tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAatc                                                                >  2:1360976/1‑109 (MQ=255)
                                                                                                                             tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAatc                                                                <  1:1360976/109‑1 (MQ=255)
                                                                                                                             tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc                              >  4:399077/1‑143 (MQ=255)
                                                                                                                             tCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc                              <  3:399077/143‑1 (MQ=255)
                                                                                                                             gaaccGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGac                        >  2:102005/4‑149 (MQ=255)
                                                                                                                                   tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGTCACGCTGCCGCGCAGGGACACTCGc                  <  2:1850257/149‑1 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGcc                                                                              <  2:288285/85‑1 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGcc                                                                              >  1:288285/1‑85 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgac              <  2:1457811/149‑1 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgac              >  3:614714/1‑149 (MQ=255)
                                                                                                                                         ggATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgactg            >  3:5980/1‑149 (MQ=255)
                                                                                                                                           aTAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgac              >  2:1005080/1‑145 (MQ=255)
                                                                                                                                           aTAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGctgac              <  1:1005080/145‑1 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGacac                      <  2:347715/130‑1 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGacac                      >  1:347715/1‑130 (MQ=255)
                                                                                                                                                   tttACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCg  >  4:1112354/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCG  >  NC_000913/2340231‑2340526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: