Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,375,484 A→T 9.3% L160H (CTT→CAT) ‡ menC ← O‑succinylbenzoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,4840AT 9.3% 477.6 / 32.2 140L160H (CTT→CAT) ‡menCO‑succinylbenzoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (65/62);  new base T (7/6);  total (72/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

Only 100 of 149 total aligned reads displayed.

GGCAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATT  >  NC_000913/2375336‑2375632
                                                                                                                                                    |                                                                                                                                                    
ggcaatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      <  1:640884/149‑1 (MQ=255)
ggcaatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      <  3:1145265/149‑1 (MQ=255)
  caatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGa                                                                                                                                                    >  2:117319/1‑149 (MQ=255)
   aatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGTACGGCTCTTCGAGAAACGCGATGCGATCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                   >  4:1550695/1‑149 (MQ=255)
    atgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACg                                                                                                                                                  >  3:199581/1‑149 (MQ=255)
         ggTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCTTGACGCAAAt                                                                                                                                             <  4:1225001/149‑1 (MQ=255)
         ggTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAAt                                                                                                                                             <  2:1849553/149‑1 (MQ=255)
          gTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATg                                                                                                                                            >  1:1518047/1‑149 (MQ=255)
               aCGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGAt                                                                                                                                       <  2:46016/149‑1 (MQ=255)
                 gggCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGc                                                                                                                                           >  4:172336/1‑143 (MQ=255)
                 gggCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGc                                                                                                                                           <  3:172336/143‑1 (MQ=255)
                 gggCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATcc                                                                                                                                     >  4:1666609/1‑149 (MQ=255)
                     aaaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      <  4:706365/128‑1 (MQ=255)
                     aaaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      >  3:706365/1‑128 (MQ=255)
                     aaaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCgg                                                                                                                                   <  2:100006/147‑1 (MQ=255)
                     aaaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCgg                                                                                                                                   >  1:100006/1‑147 (MQ=255)
                              cgAATCATCGCGGGTTTTGTACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCa                                                                                                                        <  3:1550695/149‑1 (MQ=255)
                               gAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAg                                                                                                                       <  1:1498250/149‑1 (MQ=255)
                                aaTCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      >  2:633234/1‑117 (MQ=255)
                                aaTCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                      <  1:633234/117‑1 (MQ=255)
                                   catcGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAAc                                                                                                                   <  3:1666609/149‑1 (MQ=255)
                                     tcGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAAt                                                                                                                                >  3:1526642/1‑134 (MQ=255)
                                     tcGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAAt                                                                                                                                <  4:1526642/134‑1 (MQ=255)
                                     tagcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCAGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGGGTCAAGACGCAAATGGAGATCCGGAATTGCCTCCAGCAACAg                                                                                                                 >  1:52668/3‑149 (MQ=255)
                                          ggTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTca                                                                                                            >  2:735104/1‑149 (MQ=255)
                                          ggTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTca                                                                                                            >  2:1825921/1‑149 (MQ=255)
                                                gCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGGTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCg                                                                                                                                    >  4:1293489/1‑119 (MQ=255)
                                                gCACGCCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCg                                                                                                                                    <  3:1293489/119‑1 (MQ=255)
                                                  aCGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATg                                                                                                    >  4:917850/1‑149 (MQ=255)
                                                   cGGCTCTTCGAGAAACGCGGTGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGc                                                                                                   >  1:1123691/1‑149 (MQ=255)
                                                       tctTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATcc                                                                                                                                     >  4:1187728/1‑111 (MQ=255)
                                                       tctTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATcc                                                                                                                                     <  3:1187728/111‑1 (MQ=255)
                                                        ctTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcg                                                                                              <  1:1071970/149‑1 (MQ=255)
                                                         ttagagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                             >  2:1794757/4‑149 (MQ=255)
                                                          tCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTc                                                                                                                          >  3:1796634/1‑119 (MQ=255)
                                                          tCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTc                                                                                                                          <  4:1796634/119‑1 (MQ=255)
                                                            gagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCAccacca                                                                                                      <  4:777953/137‑1 (MQ=255)
                                                            gagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCAccacca                                                                                                      >  3:777953/1‑137 (MQ=255)
                                                                 aCGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATCCACCACCATGCCGTCGCGCACCGCt                                                                                     >  4:146609/1‑149 (MQ=255)
                                                                      aTGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTa                                                                                >  2:324880/1‑149 (MQ=255)
                                                                      aTGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTa                                                                                >  4:1893711/1‑149 (MQ=255)
                                                                       tGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                   >  1:1377493/1‑81 (MQ=255)
                                                                                gATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACt                                                                      >  2:774723/1‑149 (MQ=255)
                                                                                gATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACt                                                                      >  1:839719/1‑149 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGa                                                                  <  1:1825921/149‑1 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGa                                                                  <  1:735104/149‑1 (MQ=255)
                                                                                     tCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGAc                                                                 <  4:199581/149‑1 (MQ=255)
                                                                                            tAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGcc                                                          <  1:1196815/149‑1 (MQ=255)
                                                                                              aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                        >  2:479851/1‑149 (MQ=255)
                                                                                                 tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGTAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCTCACTTTGACCTTCGCCACttt                                                     >  1:1393822/1‑149 (MQ=255)
                                                                                                 tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACttt                                                     >  1:346363/1‑149 (MQ=255)
                                                                                                   tttGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtct                                                   >  1:1169156/1‑149 (MQ=255)
                                                                                                   tttGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtct                                                   <  2:1420171/149‑1 (MQ=255)
                                                                                                    ttGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtctc                                                  >  1:144644/1‑149 (MQ=255)
                                                                                                      ggCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtct                                                   <  1:1431457/146‑1 (MQ=255)
                                                                                                       gCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGTAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCTCACTTTGACCTTCGCCACTTTCTCGcc                                               <  2:1393822/149‑1 (MQ=255)
                                                                                                         aaaCTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTg                                             >  1:1092941/1‑149 (MQ=255)
                                                                                                                 gACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCt                                     >  4:1187555/1‑149 (MQ=255)
                                                                                                                   ccTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGc                                   <  2:1488289/149‑1 (MQ=255)
                                                                                                                    cTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtctc                                                  >  3:1296019/1‑133 (MQ=255)
                                                                                                                    cTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCa                                  >  2:229172/1‑149 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTc                                                             <  2:1556380/121‑1 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                 >  3:885414/1‑149 (MQ=255)
                                                                                                                         aGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGttt                             >  1:1582799/1‑149 (MQ=255)
                                                                                                                           cGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtc                                                    <  3:595331/124‑1 (MQ=255)
                                                                                                                           cGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtc                                                    >  4:595331/1‑124 (MQ=255)
                                                                                                                            ggTGTCCCGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCACGCACCGCTTCGTACAATCCCGCCTTCACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                          <  2:52668/149‑1 (MQ=255)
                                                                                                                            ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                          <  3:1125408/149‑1 (MQ=255)
                                                                                                                            ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                          <  4:1121732/149‑1 (MQ=255)
                                                                                                                            ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                          <  2:346363/149‑1 (MQ=255)
                                                                                                                             gtgtCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAgg                         <  1:229172/149‑1 (MQ=255)
                                                                                                                               gtCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGAt                       <  2:1210121/149‑1 (MQ=255)
                                                                                                                                tCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATc                      <  3:1187555/149‑1 (MQ=255)
                                                                                                                                tCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATc                      >  1:728111/1‑149 (MQ=255)
                                                                                                                                 ccAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAATAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                     <  1:324880/149‑1 (MQ=255)
                                                                                                                                 ccAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                     <  3:1893711/149‑1 (MQ=255)
                                                                                                                                 ccAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                     <  2:653052/149‑1 (MQ=255)
                                                                                                                                   aGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                   >  1:1208362/1‑149 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTc                                                             >  1:476000/1‑100 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTc                                                             <  2:476000/100‑1 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAgg                         <  4:87030/136‑1 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                     <  4:1771068/140‑1 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCa                     >  3:1771068/1‑140 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg            >  2:1125759/1‑149 (MQ=255)
                                                                                                                                          aTTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg            <  1:479851/149‑1 (MQ=255)
                                                                                                                                          aTTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg            <  2:976134/149‑1 (MQ=255)
                                                                                                                                           tttGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCgg           >  2:1563897/1‑149 (MQ=255)
                                                                                                                                           tttGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCgg           >  4:1520998/1‑149 (MQ=255)
                                                                                                                                             tGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTc              >  1:187529/1‑144 (MQ=255)
                                                                                                                                             tGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGAt         <  1:1563897/149‑1 (MQ=255)
                                                                                                                                             tGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGAt         <  2:1208362/149‑1 (MQ=255)
                                                                                                                                              gCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                   >  2:996602/1‑138 (MQ=255)
                                                                                                                                              gCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                   <  1:996602/138‑1 (MQ=255)
                                                                                                                                              gCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATc        <  1:1794757/149‑1 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCCCTTTGACCTTCGCCACTTTCTCGCCTGGCATATc                                      <  3:584763/117‑1 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATc                                      >  4:584763/1‑117 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATc                                      >  2:531545/1‑117 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATc                                      <  1:531545/117‑1 (MQ=255)
                                                                                                                                                  cAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCa    <  2:144644/149‑1 (MQ=255)
                                                                                                                                                    aGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAtt  >  1:835982/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCATT  >  NC_000913/2375336‑2375632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: