Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,425,975 A→G 7.1% intergenic (‑59/+31) hisQ ← / ← hisJ histidine ABC transporter permease/histidine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,425,9750AG 7.1% 551.1 / 23.1 169intergenic (‑59/+31)hisQ/hisJhistidine ABC transporter permease/histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (78/79);  new base G (7/5);  total (85/84)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

Only 100 of 178 total aligned reads displayed.

ATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTATCTTCTT  >  NC_000913/2425828‑2426121
                                                                                                                                                   |                                                                                                                                                  
atgatTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                                   <  4:487438/149‑1 (MQ=255)
    ttACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAgg                                                                                                                                                 >  4:1859735/1‑147 (MQ=255)
    ttACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa                                                                                                                                               >  2:1450365/1‑149 (MQ=255)
    ttACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGa                                                                                                                                               <  1:29672/149‑1 (MQ=255)
        aGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCgg                                                                                                                                           <  1:1155608/149‑1 (MQ=255)
         gCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                                   <  2:1791302/140‑1 (MQ=255)
         gCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                                   <  2:657113/140‑1 (MQ=255)
         gCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGaa                                                                                                                                                   >  1:1791302/1‑140 (MQ=255)
         gCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCggg                                                                                                                                          >  1:686351/1‑149 (MQ=255)
            aGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctt                                                                                                                                       >  2:1569995/1‑149 (MQ=255)
                 tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAg                                                                                                                                                  <  3:622590/133‑1 (MQ=255)
                 tACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttcttt                                                                                                                                  >  2:1473083/1‑149 (MQ=255)
                    agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     >  4:1682734/1‑143 (MQ=255)
                    agagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     <  3:1682734/143‑1 (MQ=255)
                     gagCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCAcc                                                                                                                              <  2:451528/149‑1 (MQ=255)
                       gCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                               <  3:1294696/130‑1 (MQ=255)
                       gCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTTCGGGTAACCGGAGGGa                                                                                                                                               >  4:1294696/1‑130 (MQ=255)
                         tGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                          <  3:1500135/149‑1 (MQ=255)
                           aTAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGc                                                                                                                        >  1:575161/1‑149 (MQ=255)
                           aTAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGc                                                                                                                        <  1:1450365/149‑1 (MQ=255)
                              gCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAt                                                                                                                     >  1:1148207/1‑149 (MQ=255)
                               ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCa                                                                                                                                >  3:336815/1‑137 (MQ=255)
                               ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCa                                                                                                                                <  4:336815/137‑1 (MQ=255)
                               ccAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGAtt                                                                                                                    <  1:490156/149‑1 (MQ=255)
                                 aGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa                                                                                                                   <  2:1711828/148‑1 (MQ=255)
                                 aGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa                                                                                                                   >  1:1711828/1‑148 (MQ=255)
                                  gCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGc                                                                                                                 <  2:1366845/149‑1 (MQ=255)
                                  gCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGc                                                                                                                 <  1:939671/149‑1 (MQ=255)
                                    tCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcca                                                                                                               <  4:588409/149‑1 (MQ=255)
                                      cAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCATGTGTGGTGAATTACCGCCTCTTGTCTCCCCTCGGGTAACCGGAGGGa                                                                                                                                               >  3:1187878/1‑115 (MQ=255)
                                          gTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATaa                                                                                                         >  2:1257363/1‑149 (MQ=255)
                                           tGACGAGCGCACCCTGTAAAATAACACCTGAATACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATaaa                                                                                                        <  3:597298/149‑1 (MQ=255)
                                           tGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATaaa                                                                                                        <  2:1188809/149‑1 (MQ=255)
                                           tGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATaaa                                                                                                        <  2:1148207/149‑1 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCGATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     <  1:544598/110‑1 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     >  2:544598/1‑110 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     <  3:319458/110‑1 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttc                                                                                                                                     >  4:319458/1‑110 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTa                                                                                                                   >  1:1042399/1‑128 (MQ=255)
                                                     aCCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCCATTAGcca                                                                                                               <  3:1742943/132‑1 (MQ=255)
                                                        cTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGa                                                                                                                      <  4:517678/122‑1 (MQ=255)
                                                         tGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTTACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAg                                                                                          >  3:1132421/1‑149 (MQ=255)
                                                         tGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                           <  1:1356957/148‑1 (MQ=255)
                                                          gTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                           <  2:939377/147‑1 (MQ=255)
                                                           tAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGcc                                                                                                                >  4:1454885/1‑125 (MQ=255)
                                                               aTAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACttt                                                                                    >  1:1591454/1‑149 (MQ=255)
                                                                tAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACtttt                                                                                   <  1:1473083/149‑1 (MQ=255)
                                                                  acacCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                          >  2:1543287/1‑108 (MQ=255)
                                                                  acacCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATg                                                                                                                          <  1:1543287/108‑1 (MQ=255)
                                                                     ccTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCt                                                                              >  3:499457/1‑149 (MQ=255)
                                                                      cTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                                    >  3:1166164/1‑94 (MQ=255)
                                                                      cTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGctttct                                                                                                                                    <  4:1166164/94‑1 (MQ=255)
                                                                        gAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAt                                                                           >  2:658465/1‑149 (MQ=255)
                                                                        gAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAAt                                                                           >  4:1769906/1‑149 (MQ=255)
                                                                             cccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                                      <  4:1249079/149‑1 (MQ=255)
                                                                              ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGa                                                                                                                      <  4:376504/100‑1 (MQ=255)
                                                                              ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGa                                                                                                                      >  3:376504/1‑100 (MQ=255)
                                                                              ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCg                                                                     >  2:79493/1‑149 (MQ=255)
                                                                              ccATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCg                                                                     >  4:1842168/1‑149 (MQ=255)
                                                                                 tACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCtttctttc                                                                                                                                 >  3:1888304/1‑86 (MQ=255)
                                                                                 tACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTaa                                                                  <  4:1132421/149‑1 (MQ=255)
                                                                                 tACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTaa                                                                  <  3:1769906/149‑1 (MQ=255)
                                                                                 tACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTaa                                                                  <  3:1842168/149‑1 (MQ=255)
                                                                                  acaacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACttttt                                                                                  <  2:132024/132‑1 (MQ=255)
                                                                                    aacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGc                                                                                                                                         >  3:755085/1‑75 (MQ=255)
                                                                                    aacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGc                                                                                                                                         <  4:755085/75‑1 (MQ=255)
                                                                                    aacaTGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGAt                                                                                                                           >  1:1439317/1‑89 (MQ=255)
                                                                                         gctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCaaa                                                                                                 <  1:1306168/110‑1 (MQ=255)
                                                                                         gctgcCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCaaa                                                                                                 >  2:1306168/1‑110 (MQ=255)
                                                                                              ctgtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCATCATAAACATCAAAATCGAAGTACTTTTTCGc                                                                               >  3:229486/1‑123 (MQ=255)
                                                                                              ctgtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCATCATAAACATCAAAATCGAAGTACTTTTTCGc                                                                               <  4:229486/123‑1 (MQ=255)
                                                                                                gtcctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAgcgcgc                                                   <  1:802224/149‑1 (MQ=255)
                                                                                                  cctgtcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAt                                                 <  1:1257363/149‑1 (MQ=255)
                                                                                                      tcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAttt                                               <  3:16700/147‑1 (MQ=255)
                                                                                                      tcGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAttt                                               >  4:16700/1‑147 (MQ=255)
                                                                                                         tgtgtgGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                           >  3:528460/1‑100 (MQ=255)
                                                                                                            gtggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGt                                                           <  4:619941/129‑1 (MQ=255)
                                                                                                              ggtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAg                                       >  2:114238/1‑147 (MQ=255)
                                                                                                               gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCt                                    >  1:1193043/1‑149 (MQ=255)
                                                                                                               gtgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCt                                    >  2:1006886/1‑149 (MQ=255)
                                                                                                                tgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCtt                                   <  2:373727/149‑1 (MQ=255)
                                                                                                                tgAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCtt                                   <  3:1359186/149‑1 (MQ=255)
                                                                                                                 gAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCttt                                  <  1:1218578/149‑1 (MQ=255)
                                                                                                                  aaTTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTg                                 >  3:659251/1‑149 (MQ=255)
                                                                                                                             ctTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTc                      <  1:1006886/149‑1 (MQ=255)
                                                                                                                              ttGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCTAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCAt                                                 >  4:1174086/1‑121 (MQ=255)
                                                                                                                                gTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTcgcg                   >  3:1438463/1‑149 (MQ=255)
                                                                                                                                 tctcCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCgcgc                  >  1:477544/1‑149 (MQ=255)
                                                                                                                                 gctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCgcgc                  >  2:898953/2‑149 (MQ=255)
                                                                                                                                  ctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                           <  3:691654/75‑1 (MQ=37)
                                                                                                                                  ctcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGaa                                                                                           >  4:691654/1‑75 (MQ=37)
                                                                                                                                   tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAg                            <  4:1406771/137‑1 (MQ=255)
                                                                                                                                   tcccttcGGGTAACCGGAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAg                >  2:337249/1‑149 (MQ=255)
                                                                                                                                               cccGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAtcttc    <  3:425691/149‑1 (MQ=255)
                                                                                                                                                ccGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAtcttct   >  1:657709/1‑149 (MQ=255)
                                                                                                                                                ccGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAtcttct   >  3:1067598/1‑149 (MQ=255)
                                                                                                                                                ccGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTAtcttct   >  2:419908/1‑149 (MQ=255)
                                                                                                                                                 cGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTATcttctt  >  4:401726/1‑149 (MQ=255)
                                                                                                                                                 cGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTATcttctt  <  4:659251/149‑1 (MQ=255)
                                                                                                                                                   aaGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTtctc                                                                      >  1:398765/1‑79 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGATTACAGCGAGCACTACAGAGCTGATAGCCAGCTCCAGCGTGACGAGCGCACCCTGTAAAATAACACCTGAAAACCCATACAACATGCTGCCTGTCCTGTCGTGTGTGGTGAATTACCGCCTCTTGTCTCCCTCCGGTTACCCGAAGGGAGCGGGCTTTCTTTCACCGATGGCGATTAGCCACCATAAACATCAAAATCGAAGTACTTTTTCGCTAATTTCTCGTAAGTACCGTCAGCGCGCATTTCGGCAAAGGCTTTGTTCAGTGCTTCGCGCAGTTCGTTATCTTCTT  >  NC_000913/2425828‑2426121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: