Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,493,563 T→A 9.7% intergenic (‑309/+204) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,493,5630TA 9.7% 349.7 / 23.2 103intergenic (‑309/+204)frc/yfdXformyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (41/52);  new base A (4/6);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 107 total aligned reads displayed.

TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCA  >  NC_000913/2493415‑2493707
                                                                                                                                                    |                                                                                                                                                
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                  >  1:142212/1‑149 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               <  1:291489/149‑1 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               <  2:1757496/149‑1 (MQ=255)
       aTGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGt                                                                                                                                           <  1:1779695/149‑1 (MQ=255)
        tGGTAAGTAGTGAGGACGAAAAGAAGAATATGATCAATATTGATTCGTTTTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTGACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTa                                                                                                                                          <  2:105013/149‑1 (MQ=255)
          gTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTAcc                                                                                                                                        >  3:1834512/1‑149 (MQ=255)
          gTAAGTAGTGAGGACAAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                        <  4:382634/149‑3 (MQ=255)
           tAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               >  2:1651157/1‑141 (MQ=255)
           tAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               <  1:1651157/141‑1 (MQ=255)
                  tGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaaca                                                                                                                                >  3:1340102/1‑149 (MQ=255)
                    aGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacata                                                                                                                              <  2:1172440/149‑1 (MQ=255)
                    aGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                        >  4:1639156/1‑137 (MQ=255)
                    aGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                        <  3:1639156/139‑3 (MQ=255)
                       aCGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAATAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataaca                                                                                                                           >  4:1078834/1‑149 (MQ=255)
                          aaaaGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAAcataacataa                                                                                                                        <  1:490655/149‑1 (MQ=255)
                             agaagaATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACAtaataa                                                                                                                     >  3:1214890/1‑149 (MQ=255)
                             agaagaATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                        >  3:968908/1‑128 (MQ=255)
                               aagaaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATaaaa                                                                                                                                  <  3:381245/134‑1 (MQ=255)
                               aagaaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATaaaa                                                                                                                                  >  4:381245/1‑134 (MQ=255)
                                 gaaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTTATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGa                                                                                                                 <  1:515236/149‑1 (MQ=255)
                                      tGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGTTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            <  1:1347669/149‑1 (MQ=255)
                                      tGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            <  4:1214890/149‑1 (MQ=255)
                                       gATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgtg                                                                                                           <  3:1078834/149‑1 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAAt                                                                                                                   >  1:461726/1‑140 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAAt                                                                                                                   <  2:461726/140‑1 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                          <  1:539686/149‑1 (MQ=255)
                                             tatTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGaca                                                                                                     <  2:180604/149‑1 (MQ=255)
                                             tatTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGaca                                                                                                     >  1:1109733/1‑149 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               >  4:858642/1‑102 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                               <  3:858642/102‑1 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            >  3:241787/1‑137 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            <  4:241787/137‑1 (MQ=255)
                                                   ttCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGat                                                                                               <  1:71877/149‑1 (MQ=255)
                                                       tGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGattatt                                                                                           >  1:1847112/1‑149 (MQ=255)
                                                             tCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGtt                                                                                     >  3:13425/1‑149 (MQ=255)
                                                               agagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                             <  3:319781/107‑1 (MQ=255)
                                                               agagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                             >  4:319781/1‑107 (MQ=255)
                                                               agagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtgat                                                                                                         <  3:40641/127‑1 (MQ=255)
                                                               agagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtgat                                                                                                         >  4:40641/1‑127 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGa                                                                                                                 <  1:833796/118‑1 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGa                                                                                                                 >  2:833796/1‑118 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTAc                                                                                  >  3:1870122/1‑149 (MQ=255)
                                                                     agTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                             <  4:1834512/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  4:13425/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  2:142212/149‑1 (MQ=255)
                                                                            aCATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAatat                                                                      >  2:801659/1‑149 (MQ=255)
                                                                              aTTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaa                                                                    >  3:1013340/1‑149 (MQ=255)
                                                                                  gtgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTAc                                                                                                                                         >  4:1289903/1‑76 (MQ=255)
                                                                                  gtgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTAc                                                                                                                                         <  3:1289903/76‑1 (MQ=255)
                                                                                     aTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                             <  4:1340102/149‑1 (MQ=255)
                                                                                     aTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                             <  4:1013340/149‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            >  3:605560/1‑132 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  4:605560/132‑1 (MQ=255)
                                                                                        ttATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGtt                                                          <  2:1847112/149‑1 (MQ=255)
                                                                                          cttCTTACAGTTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  1:1163483/128‑1 (MQ=255)
                                                                                          cttCTTACAGTTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            >  2:1163483/2‑129 (MQ=255)
                                                                                          aTTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                             >  3:848406/1‑128 (MQ=255)
                                                                                          aTTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                             <  4:848406/128‑1 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTa                                                                                                                                          >  1:1127120/1‑66 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTa                                                                                                                                          <  2:1127120/66‑1 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGTGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                       <  3:518634/146‑1 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGatta                                                                                             <  4:509742/108‑1 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGatta                                                                                             >  3:509742/1‑108 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                             <  1:1511357/124‑1 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGa                                                                             >  2:1511357/1‑124 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                       >  4:518634/1‑146 (MQ=255)
                                                                                                aCAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaaaa                                                                  <  2:398983/133‑1 (MQ=255)
                                                                                                aCAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaaaa                                                                  >  1:398983/1‑133 (MQ=255)
                                                                                                  aaCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                          <  1:283516/91‑1 (MQ=255)
                                                                                                  aaCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                          >  2:283516/1‑91 (MQ=255)
                                                                                                  aaCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaa                                                                                                                                         >  2:1058368/1‑59 (MQ=39)
                                                                                                  aaCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaa                                                                                                                                         <  1:1058368/60‑2 (MQ=39)
                                                                                                   aCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            >  4:1349813/1‑88 (MQ=255)
                                                                                                   aCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                            <  3:1349813/88‑1 (MQ=255)
                                                                                                    cTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtata                                                      >  1:1796616/1‑141 (MQ=255)
                                                                                                    cTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtata                                                      <  2:1796616/141‑1 (MQ=255)
                                                                                                         agaagCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGa                                                                                                <  1:1076957/94‑1 (MQ=255)
                                                                                                             gcgcCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCaaa                                     <  2:1109733/149‑1 (MQ=255)
                                                                                                                            aagcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                     <  1:919303/118‑1 (MQ=255)
                                                                                                                            aagcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                     >  2:919303/1‑118 (MQ=255)
                                                                                                                             agcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACca                       <  4:1430685/147‑1 (MQ=255)
                                                                                                                             agcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACca                       >  3:1430685/1‑147 (MQ=255)
                                                                                                                              gcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTa                                                                                   >  1:379074/1‑86 (MQ=255)
                                                                                                                              gcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTa                                                                                   <  2:379074/86‑1 (MQ=255)
                                                                                                                                   ggTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            >  1:1879075/1‑88 (MQ=255)
                                                                                                                                   ggTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  2:1879075/88‑1 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGcc                                        >  4:114878/1‑123 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGcc                                        <  3:114878/123‑1 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc              <  1:801659/149‑1 (MQ=255)
                                                                                                                                         aTTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGaca                                                                                                     <  2:1768904/57‑1 (MQ=255)
                                                                                                                                         aTTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGaca                                                                                                     >  1:1768904/1‑57 (MQ=255)
                                                                                                                                          ttACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGttt                                                         >  4:30605/1‑100 (MQ=255)
                                                                                                                                          ttACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGttt                                                         <  3:30605/100‑1 (MQ=255)
                                                                                                                                           tACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTTCGGTTTACTTTGAGGAATATAAAATGCGAGTTTTTAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtgt       >  4:634408/1‑149 (MQ=255)
                                                                                                                                           tACGCTTTAAAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAg                                            <  1:1675256/112‑1 (MQ=255)
                                                                                                                                           tACGCTTTAAAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAg                                            >  2:1675256/1‑112 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTAtt         <  4:416802/144‑1 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTAtt         >  3:416802/1‑144 (MQ=255)
                                                                                                                                                  tttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            <  3:280744/73‑1 (MQ=255)
                                                                                                                                                  tttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                            >  4:280744/1‑73 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCA  >  NC_000913/2493415‑2493707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: