Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,549,533 G→C 7.4% intergenic (+127/+113) yfeW → / ← yfeX penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,549,5330GC 7.4% 280.1 / 12.3 81intergenic (+127/+113)yfeW/yfeXpenicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base C (3/3);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

GGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGA  >  NC_000913/2549388‑2549680
                                                                                                                                                 |                                                                                                                                                   
ggcgTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc                                                                                                                                                  <  1:725220/149‑1 (MQ=255)
     tAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATa                                                                                                                                             >  1:1829085/1‑149 (MQ=255)
       aaGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAg                                                                                                                                           <  2:1829085/149‑1 (MQ=255)
       aaGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAg                                                                                                                                           <  3:1605872/149‑1 (MQ=255)
         gCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGAtgcggc                                                                                                                                         >  4:1469586/1‑144 (MQ=255)
         gCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGc                                                                                                                                         >  4:457236/1‑149 (MQ=255)
         gCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGAtgcggc                                                                                                                                         >  3:215901/1‑144 (MQ=255)
           aGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGt                                                                                                                                       <  3:457236/149‑1 (MQ=255)
                 aataaAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                                 <  2:1678706/149‑1 (MQ=255)
                      aaaGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAAcgccttat                                                                                                                            <  3:1469586/149‑5 (MQ=37)
                       aaGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAAcgccttatc                                                                                                                           <  4:1877683/149‑6 (MQ=25)
                         ggCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                         >  3:686889/1‑149 (MQ=255)
                             agtcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa                                                                                                                                    <  4:1259019/134‑1 (MQ=255)
                             agtcagtAATGTTACTGGCTGGTCTGAGAGTGCTAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTAGTAGGCCGGATAAGGCGTTCa                                                                                                                                    >  3:1259019/1‑134 (MQ=255)
                              gtcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGATGCGGCGTGAACGCCTTATCCGGccta                                                                                                                    <  4:215901/149‑4 (MQ=25)
                               tcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgtgt                                                                                                                   >  3:1086059/1‑149 (MQ=255)
                                  gtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGAAGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACa                                                                                                                >  2:557395/1‑149 (MQ=255)
                                    aaTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAAc                                                                                                              <  1:557395/149‑1 (MQ=255)
                                    aaTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGATGCGGCGTGAACGCCTTATCCGGcct                                                                                                                     >  1:964920/1‑140 (MQ=25)
                                    aaTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGATGCGGCGTGAACGCCTTATCCGGcct                                                                                                                     <  2:1005022/142‑3 (MQ=21)
                                    aaTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGATGCGGCGTGAACGCCTTATCCGGcct                                                                                                                     <  2:964920/142‑3 (MQ=21)
                                    aaTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTACGCCGGATGCGGCGTGAACGCCTTATCCGGcct                                                                                                                     >  1:1005022/1‑140 (MQ=21)
                                     aTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGt                                                                                                                                       >  4:1058773/1‑123 (MQ=255)
                                     aTGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGt                                                                                                                                       <  3:1058773/123‑1 (MQ=255)
                                                tggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAAcgccttat                                                                                                                            >  3:937863/1‑119 (MQ=21)
                                                tggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAAcgccttat                                                                                                                            <  4:937863/123‑5 (MQ=21)
                                                    cTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGt                                                                                                   <  4:566082/144‑1 (MQ=255)
                                                    cTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGt                                                                                                   >  3:566082/1‑144 (MQ=255)
                                                     tGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTcaacaa                                                                                             <  4:686889/149‑1 (MQ=255)
                                                                aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc                                                                                                                                                  >  4:795489/1‑85 (MQ=255)
                                                                aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc                                                                                                                                                  <  3:795489/85‑1 (MQ=255)
                                                                  aaaCGCAAAACTGCCTGATGCGTTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagtc                                                                                >  1:1551275/1‑149 (MQ=255)
                                                                  aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                       <  4:1240617/110‑1 (MQ=18)
                                                                  aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                       >  3:1240617/1‑110 (MQ=18)
                                                                   aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAAcgc                                                                                                            >  4:1834979/1‑120 (MQ=255)
                                                                   aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAAcgc                                                                                                            <  3:1834979/120‑1 (MQ=255)
                                                                      gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                      <  4:744010/107‑2 (MQ=18)
                                                                             tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTAc                                                                     <  2:1551275/149‑1 (MQ=255)
                                                                                     gcgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc                                                                                                                                                  <  4:1807577/64‑1 (MQ=255)
                                                                                     gcgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGcc                                                                                                                                                  >  3:1807577/1‑64 (MQ=255)
                                                                                         tACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGc                                                             <  2:1662753/145‑1 (MQ=255)
                                                                                         tACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGc                                                             >  1:1662753/1‑145 (MQ=255)
                                                                                           cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTaa                                                       <  4:1086059/149‑1 (MQ=255)
                                                                                            gCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt                                                      >  4:108238/1‑149 (MQ=255)
                                                                                              ttATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCa                                                    >  4:1464003/1‑149 (MQ=255)
                                                                                               tATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                   <  3:1464003/149‑1 (MQ=255)
                                                                                                aTCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                         >  1:1486608/1‑78 (MQ=255)
                                                                                                aTCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                         <  2:1486608/78‑1 (MQ=255)
                                                                                                aTCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAAt                                                  <  3:108238/149‑1 (MQ=255)
                                                                                                       cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa                                                                                                                                    >  2:349471/1‑60 (MQ=255)
                                                                                                       cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCa                                                                                                                                    <  1:349471/60‑1 (MQ=255)
                                                                                                       cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAAt                                              >  3:1614508/1‑146 (MQ=255)
                                                                                                       cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAAt                                              <  4:1614508/146‑1 (MQ=255)
                                                                                                              gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCt                                    >  2:427333/1‑149 (MQ=255)
                                                                                                                 cccACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt                                                          <  1:1817793/124‑1 (MQ=255)
                                                                                                                 cccACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt                                                          >  2:1817793/1‑124 (MQ=255)
                                                                                                                    acaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTaacat                                                                        >  3:1151658/1‑104 (MQ=255)
                                                                                                                    acaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTaacat                                                                        <  4:1151658/107‑4 (MQ=255)
                                                                                                                       atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAgcgcg                           >  2:1739573/1‑148 (MQ=255)
                                                                                                                       atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGcc                           >  4:7306/1‑149 (MQ=255)
                                                                                                                             tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGcc                                                            <  1:335562/110‑1 (MQ=255)
                                                                                                                             tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGcc                                                            >  2:335562/1‑110 (MQ=255)
                                                                                                                             tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTaacatta                                                                      <  1:1212070/100‑6 (MQ=255)
                                                                                                                             tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTaacatta                                                                      >  2:1212070/1‑95 (MQ=255)
                                                                                                                               aaTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt                   >  3:1492530/1‑149 (MQ=255)
                                                                                                                                aTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGa                                                                                        <  1:1105688/79‑1 (MQ=255)
                                                                                                                                aTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGa                                                                                        >  2:1105688/1‑79 (MQ=255)
                                                                                                                                   aGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGc                                                                                     <  2:247173/79‑1 (MQ=255)
                                                                                                                                   aGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGc                                                                                     >  1:247173/1‑79 (MQ=255)
                                                                                                                                    ggaCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTc                                                                                                  >  4:1362782/3‑65 (MQ=255)
                                                                                                                                    gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTc                                                                                                  <  3:1362782/65‑1 (MQ=255)
                                                                                                                                    gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                   >  1:1741858/1‑112 (MQ=255)
                                                                                                                                    gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                   <  2:1741858/112‑1 (MQ=255)
                                                                                                                                     cACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAg             >  1:1852173/1‑149 (MQ=255)
                                                                                                                                        gATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGa          <  1:427333/149‑1 (MQ=255)
                                                                                                                                              gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGa                                                                                        <  2:956925/65‑1 (MQ=255)
                                                                                                                                              gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGa                                                                                        >  1:956925/1‑65 (MQ=255)
                                                                                                                                                aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCGATCAACTTGTCCAGCGACGGTGCGa  <  1:1739573/149‑1 (MQ=255)
                                                                                                                                                aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGAc         >  2:199169/1‑142 (MQ=255)
                                                                                                                                                aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGAc         <  1:199169/142‑1 (MQ=255)
                                                                                                                                                aGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGa  <  3:7306/149‑1 (MQ=255)
                                                                                                                                                 ggCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGt      <  1:577393/144‑1 (MQ=255)
                                                                                                                                                 ggCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGt      >  2:577393/1‑144 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGCGTTAAAGCAGAAGTAATAAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGA  >  NC_000913/2549388‑2549680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: