BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,555,205	G→C	5.1%	intergenic (‑23/+23)	eutR ← / ← eutK	eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,205	0	G	C	5.1%	332.7 / 5.2	94	intergenic (‑23/+23)	eutR/eutK	eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand): ref base G (46/43); new base C (2/3); total (48/46)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 102 total aligned reads displayed.

TTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGT  >  NC_000913/2555057‑2555353

ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      <  3:1460611/149‑1 (MQ=255)

ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      <  3:365141/149‑1 (MQ=255)

ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      <  3:554506/149‑1 (MQ=255)

     aGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      >  4:1837366/1‑144 (MQ=255)

     aGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      <  2:1713555/144‑1 (MQ=255)

     aGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      <  3:1837366/144‑1 (MQ=255)

     aGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAg                                                                                                                                                      >  1:1713555/1‑144 (MQ=255)

       aCATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACAccc                                                                                                                                               <  4:1848958/149‑1 (MQ=255)

        cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCg                                                                                                                                              <  4:1257520/149‑1 (MQ=255)

            cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                          <  4:475240/149‑1 (MQ=255)

                       gTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                               <  4:1124151/149‑1 (MQ=255)

                            tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATg                                                                                                                          <  4:1520780/149‑1 (MQ=255)

                             gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                   >  4:1319837/1‑123 (MQ=255)

                             gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                   <  3:1319837/123‑1 (MQ=255)

                             gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                         <  4:407296/149‑1 (MQ=255)

                             gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACAccc                                                                                                                                               >  3:430853/1‑127 (MQ=255)

                                      ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                           <  1:367501/138‑1 (MQ=255)

                                      ccACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                           >  2:367501/1‑138 (MQ=255)

                                        aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAGGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTACGACAg                                                                                                                                 >  2:1428524/1‑130 (MQ=255)

                                        aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAg                                                                                                                                 <  1:1428524/130‑1 (MQ=255)

                                         cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                           <  1:1603833/135‑1 (MQ=255)

                                         cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAAt                                                                                                                           >  2:1603833/1‑135 (MQ=255)

                                          ttCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCGTGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                            >  1:1501834/1‑117 (MQ=255)

                                          ttCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGc                                                                                                                                            <  2:1501834/117‑1 (MQ=255)

                                                cTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                      >  1:197603/1‑117 (MQ=255)

                                                cTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTcc                                                                                                                                      <  2:197603/117‑1 (MQ=255)

                                                 ttCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                         >  3:301667/1‑113 (MQ=255)

                                                 ttCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                         <  4:301667/113‑1 (MQ=255)

                                                 ttCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGact                                                                                                     >  3:553349/1‑149 (MQ=255)

                                                    ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGactact                                                                                                  <  4:1747536/149‑1 (MQ=255)

                                                          aGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGttt                                                                                            >  2:1271112/1‑149 (MQ=255)

                                                             ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                                    <  2:582860/90‑1 (MQ=255)

                                                             ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGa                                                                                                                                                    >  1:582860/1‑90 (MQ=255)

                                                             ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                         >  2:1522016/1‑149 (MQ=255)

                                                             ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCg                                                                                                                                              <  4:495724/96‑1 (MQ=255)

                                                             ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCg                                                                                                                                              >  3:495724/1‑96 (MQ=255)

                                                              tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                                                                        <  4:553349/149‑1 (MQ=255)

                                                              tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                                                                        >  3:1031143/1‑149 (MQ=255)

                                                              tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                                                                        <  1:1522016/149‑1 (MQ=255)

                                                               cAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                           >  3:850866/1‑129 (MQ=255)

                                                               cAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                           <  4:850866/129‑1 (MQ=255)

                                                               cAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                       >  4:1794826/1‑149 (MQ=255)

                                                                aGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTaa                                                                                      >  1:298118/1‑149 (MQ=255)

                                                                 ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATg                                                                                                                          <  3:1318362/112‑1 (MQ=255)

                                                                 ggTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATg                                                                                                                          >  4:1318362/1‑112 (MQ=255)

                                                                       cGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCg                                                                               <  2:298118/149‑1 (MQ=255)

                                                                          gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACTCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCa                                                                            >  3:680495/1‑149 (MQ=255)

                                                                          gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                             <  2:443494/116‑1 (MQ=255)

                                                                          gTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                             >  1:443494/1‑116 (MQ=255)

                                                                            aaGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                          <  1:1271112/149‑1 (MQ=255)

                                                                               ggTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaaaa                                                                       <  4:680495/149‑1 (MQ=255)

                                                                                gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                   >  3:1585524/1‑104 (MQ=255)

                                                                                gTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                   <  4:1585524/104‑1 (MQ=255)

                                                                                     tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTg                                                                 >  1:1010152/1‑149 (MQ=255)

                                                                                             aTGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCGCCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCATTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                         <  3:964731/149‑1 (MQ=255)

                                                                                                gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                         <  1:1114545/82‑1 (MQ=255)

                                                                                                gTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                         >  2:1114545/1‑82 (MQ=255)

                                                                                                 tGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                               >  2:1405086/1‑75 (MQ=255)

                                                                                                 tGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                               <  1:1405086/75‑1 (MQ=255)

                                                                                                 tGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAtt                                                     <  2:1010152/149‑1 (MQ=255)

                                                                                                       ttGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGc                                               <  3:1794826/149‑1 (MQ=255)

                                                                                                        tGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                              <  4:1031143/149‑1 (MQ=255)

                                                                                                                 gggTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                               >  3:564092/1‑75 (MQ=255)

                                                                                                                 gggTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGAc                                                                                                               <  4:564092/75‑1 (MQ=255)

                                                                                                                    tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                                  >  4:335830/1‑149 (MQ=255)

                                                                                                                      tttttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcca                                >  3:199730/1‑149 (MQ=255)

                                                                                                                            atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcca                                <  3:309863/143‑1 (MQ=255)

                                                                                                                            atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcca                                >  4:309863/1‑143 (MQ=255)

                                                                                                                            atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                                  <  1:462407/141‑1 (MQ=255)

                                                                                                                            atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                                  >  2:462407/1‑141 (MQ=255)

                                                                                                                             tgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                 <  3:376432/125‑1 (MQ=255)

                                                                                                                             tgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                 >  4:376432/1‑125 (MQ=255)

                                                                                                                             tgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                         >  1:1625239/1‑149 (MQ=255)

                                                                                                                                tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaaaa                                                                       <  4:854643/100‑1 (MQ=255)

                                                                                                                                tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaaaa                                                                       >  3:854643/1‑100 (MQ=255)

                                                                                                                                 gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                                     >  1:751579/1‑133 (MQ=255)

                                                                                                                                 gTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGc                                     <  2:751579/133‑1 (MQ=255)

                                                                                                                                   tAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCa                   >  1:336856/1‑149 (MQ=255)

                                                                                                                                     aTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGc                 <  2:336856/149‑1 (MQ=255)

                                                                                                                                        ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                                  <  1:1312497/129‑1 (MQ=255)

                                                                                                                                        ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgc                                  >  2:1312497/1‑129 (MQ=255)

                                                                                                                                          gggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACtt            >  4:363537/1‑149 (MQ=255)

                                                                                                                                          gggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACtt            >  1:1763548/1‑149 (MQ=255)

                                                                                                                                                 gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                           <  2:502055/95‑1 (MQ=255)

                                                                                                                                                 gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                           >  1:502055/1‑95 (MQ=255)

                                                                                                                                                 gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGc     >  1:779678/1‑149 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                          >  2:344430/1‑127 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                          <  1:344430/127‑1 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTg                       <  2:1725770/130‑1 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTg                       >  1:1725770/1‑130 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc              <  2:1667043/139‑1 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc              >  1:1667043/1‑139 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc    <  2:779678/149‑1 (MQ=255)

                                                                                                                                                  tAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGAGGGCAGCCACTTCTCCTGcc    >  4:1713295/1‑149 (MQ=255)

                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg   >  4:1071150/1‑149 (MQ=255)

                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg   >  3:1644435/1‑149 (MQ=255)

                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCg   >  4:479132/1‑149 (MQ=255)

                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGC‑TTTTCCAGCGGCCAGCCAAAGt                        <  1:1329754/127‑1 (MQ=255)

                                                                                                                                                   aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGC‑TTTTCCAGCGGCCAGCCAAAGt                        >  2:1329754/1‑127 (MQ=255)

                                                                                                                                                    ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGt  >  2:909855/1‑149 (MQ=255)

TTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGT  >  NC_000913/2555057‑2555353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`