Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,568,199 A→G 12.6% intergenic (‑92/+125) eutH ← / ← eutG ethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,568,1990AG12.6% 226.0 / 20.9 79intergenic (‑92/+125)eutH/eutGethanolamine transporter/ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base G (5/5);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCC  >  NC_000913/2568059‑2568347
                                                                                                                                            |                                                                                                                                                    
tCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa                                                                                                                                              <  1:128964/149‑1 (MQ=255)
    gcataaGAACATCCAGGTGTAAATTGTTATTTCGTTAATTCCCCTCGGCTTTTCTCCCTGTTAGTTTGTATTTATTTGCGGATGGGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt                                                                                                                                          <  2:1450879/145‑1 (MQ=18)
    cATAAAGCACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt                                                                                                                                          <  4:608457/149‑1 (MQ=255)
    cATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt                                                                                                                                          >  1:555699/1‑149 (MQ=255)
    cATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt                                                                                                                                          <  2:475355/149‑1 (MQ=255)
    cATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGt                                                                                                                                          <  2:555699/149‑1 (MQ=255)
                            gatgatTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGcc                                                                                                                  >  3:1828953/1‑149 (MQ=255)
                                 tttCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgcg                                                                                                                                                  >  2:1503888/1‑112 (MQ=255)
                                 tttCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgcg                                                                                                                                                  <  1:1503888/112‑1 (MQ=255)
                                  ttCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                                                                                            <  4:1828953/149‑1 (MQ=255)
                                         aTTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg                                                                                                     >  2:1083379/1‑149 (MQ=255)
                                          ttCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa                                                                                                                         >  3:1381188/1‑128 (MQ=255)
                                          ttCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa                                                                                                                         <  4:1381188/128‑1 (MQ=255)
                                                tCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCgg                                                                                                                                                 <  1:1215329/98‑1 (MQ=255)
                                                tCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCgg                                                                                                                                                 >  2:1215329/1‑98 (MQ=255)
                                                 cGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                                                                                             <  4:1743031/149‑1 (MQ=255)
                                                    cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                          <  1:1083379/149‑1 (MQ=255)
                                                    cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc                                                                                           >  1:1621188/1‑148 (MQ=255)
                                                    cTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc                                                                                           <  2:1621188/148‑1 (MQ=255)
                                                         cTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa                                                                                                                                      <  1:1104637/100‑1 (MQ=255)
                                                         cTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa                                                                                                                                      >  2:1104637/1‑100 (MQ=255)
                                                          tCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa                                                                                                                                              <  3:1555266/91‑1 (MQ=255)
                                                          tCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa                                                                                                                                              >  4:1555266/1‑91 (MQ=255)
                                                           cccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTc                                                                                                                                         <  4:257891/95‑1 (MQ=255)
                                                           cccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTc                                                                                                                                         >  3:257891/1‑95 (MQ=255)
                                                                       ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTc                                                                                                                                         >  2:239504/1‑83 (MQ=255)
                                                                       ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTc                                                                                                                                         <  1:239504/83‑1 (MQ=255)
                                                                           cctgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAAt                                                                                                                             <  1:1623508‑M1/40‑1 (MQ=255)
                                                                           cctgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAAt                                                                                                                             >  2:1623508‑M1/52‑91 (MQ=255)
                                                                            ctgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGc                                                                                                                          <  1:297346‑M1/43‑1 (MQ=255)
                                                                            ctgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGc                                                                                                                          >  2:297346‑M1/51‑93 (MQ=255)
                                                                             tgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAAtt                                                                                                                            >  2:113998‑M1/50‑90 (MQ=255)
                                                                             tgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAAtt                                                                                                                            <  1:113998‑M1/41‑1 (MQ=255)
                                                                             tgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggc                                                                                                   >  4:1569287‑M1/50‑115 (MQ=255)
                                                                             tgataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggc                                                                                                   <  3:1569287‑M1/66‑1 (MQ=255)
                                                                               ataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGatgcggcgat                                                                                                >  1:237800‑M1/48‑114 (MQ=255)
                                                                               ataagatgcgccagcatcgcatcaggcctgcggggtgtgtagcgggtGTAGGCCGGATAAGGCGCGTCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGatgcggcgat                                                                                                <  2:237800‑M1/69‑3 (MQ=255)
                                                                                         ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                     >  4:1577992/1‑149 (MQ=255)
                                                                                         ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATa                                                     >  1:640995/1‑149 (MQ=255)
                                                                                          gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt                                                                         >  2:1444783/1‑128 (MQ=255)
                                                                                          gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgt                                                                         <  1:1444783/128‑1 (MQ=255)
                                                                                          gcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa                                                    >  2:73282/1‑149 (MQ=255)
                                                                                           cgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa                                                       >  3:55037/1‑145 (MQ=255)
                                                                                           cgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa                                                       <  4:55037/145‑1 (MQ=255)
                                                                                                aCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCg                                              <  1:73282/149‑1 (MQ=255)
                                                                                                    cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc                                                                                                                                >  2:1197245/1‑63 (MQ=37)
                                                                                                    cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc                                                                                                                                <  1:1197245/63‑1 (MQ=37)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                          <  2:1539625/97‑1 (MQ=255)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                          >  1:1539625/1‑97 (MQ=255)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                      <  2:640995/149‑1 (MQ=255)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                      >  2:1386995/1‑149 (MQ=255)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                      <  3:1577992/149‑1 (MQ=255)
                                                                                                        tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCACCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                      >  3:585942/1‑149 (MQ=255)
                                                                                                         ccGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTc                                     >  4:538666/1‑149 (MQ=255)
                                                                                                         ccGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTc                                     <  1:1386995/149‑1 (MQ=255)
                                                                                                             ccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                                                                                             >  4:1867999/1‑89 (MQ=255)
                                                                                                             ccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                                                                                             <  3:1867999/89‑1 (MQ=255)
                                                                                                             ccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                 >  1:682434/1‑101 (MQ=255)
                                                                                                             ccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGcc                                                                                 <  2:682434/101‑1 (MQ=255)
                                                                                                              cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                          >  1:750480/1‑91 (MQ=255)
                                                                                                              cTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc                                                                                          <  2:750480/91‑1 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc                                                                                                                >  4:443296/1‑59 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc                                                                                                                <  3:443296/59‑1 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                        >  1:660070/1‑99 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                        <  2:660070/99‑1 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                        >  4:1250659/1‑99 (MQ=255)
                                                                                                                        aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACAtgtg                                                                        <  3:1250659/99‑1 (MQ=255)
                                                                                                                                 ggCTGGATAAGACGCGGCAAGCGTCGCATCCGGCACTTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                >  1:1709086/1‑82 (MQ=255)
                                                                                                                                 ggCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGccc                                                                                <  2:1709086/82‑1 (MQ=255)
                                                                                                                                     ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGt         <  3:538666/149‑1 (MQ=255)
                                                                                                                                     ggATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGt         >  3:395059/1‑149 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                         <  2:1159225/100‑1 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg                                                         >  1:1159225/1‑100 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCaa                                          <  1:315717/115‑1 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCaa                                          >  2:315717/1‑115 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTc                                     <  4:1474806/120‑1 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTc                                     >  3:1474806/1‑120 (MQ=255)
                                                                                                                                      gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa        >  2:22016/1‑149 (MQ=255)
                                                                                                                                        tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCACCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACa      <  4:585942/149‑1 (MQ=255)
                                                                                                                                            aCGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGcc  >  3:1058066/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
TCAGCATAAAGAACATCATGATGTACATGATGATTTCGTTAATTCCCATCGCCTTTACTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCC  >  NC_000913/2568059‑2568347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: