Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,638,584 A→T 6.3% F23L (TTT→TTA bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,5840AT 6.3% 581.3 / 21.5 159F23L (TTT→TTAbamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (72/77);  new base T (5/5);  total (77/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

Only 100 of 163 total aligned reads displayed.

ACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTT  >  NC_000913/2638438‑2638728
                                                                                                                                                  |                                                                                                                                                
aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                                                                                                                                                >  2:1024383/1‑149 (MQ=255)
aCAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                                                                                                                                                >  1:1622008/1‑149 (MQ=255)
 cAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAAc                                                                                                                                               <  2:1383608/149‑1 (MQ=255)
  aaCGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACa                                                                                                                                              >  3:52045/1‑149 (MQ=255)
     gTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCg                                                                                                                                           <  1:402513/149‑1 (MQ=255)
      ttGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGa                                                                                                                                          <  3:103937/149‑1 (MQ=255)
       tGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                         <  3:388698/149‑1 (MQ=255)
                 tGCCGGATGAAGATTGGAATAGAAGTGGCCAATGCCGCAACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGCGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACCGCCGCTTa                                                                                                                               >  3:1177018/1‑149 (MQ=255)
                     ggATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAg                                                                                                                           <  1:1011548/149‑1 (MQ=255)
                      gATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAgg                                                                                                                          <  4:127464/149‑1 (MQ=255)
                      gATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAgg                                                                                                                          <  1:1342382/149‑1 (MQ=255)
                       aTGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAggg                                                                                                                         <  2:1413121/149‑1 (MQ=255)
                       aTGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAggg                                                                                                                         <  4:1874166/149‑1 (MQ=255)
                       aTGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCAGCTTAAAAggg                                                                                                                         <  3:1114392/149‑1 (MQ=255)
                             aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaagaa                                                                                                                                      >  1:163488/1‑127 (MQ=255)
                             aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaagaa                                                                                                                                      <  2:163488/130‑4 (MQ=255)
                             aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                         <  4:449920/127‑1 (MQ=255)
                             aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                         >  3:449920/1‑127 (MQ=255)
                             aTTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACgg                                                                                                                   >  3:1203640/1‑149 (MQ=255)
                              ttGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGGCATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGa                                                                                                                  >  3:1338366/1‑149 (MQ=255)
                                  aaTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGc                                                                                                              >  4:1812665/1‑149 (MQ=255)
                                   aTAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTaaaa                                                                                                                            >  4:518194/1‑134 (MQ=255)
                                      gAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAg                                                                                                               >  4:623554/1‑144 (MQ=255)
                                      gAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTc                                                                                                          <  1:781927/149‑1 (MQ=255)
                                       aaGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTcc                                                                                                         >  3:815222/1‑149 (MQ=255)
                                        aGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCt                                                                                                        >  2:1118588/1‑149 (MQ=255)
                                         gTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGTGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACGGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTg                                                                                                       >  4:155379/1‑149 (MQ=255)
                                          ttGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                         >  3:210643/1‑114 (MQ=255)
                                          ttGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTTAACAGCGaa                                                                                                                                         <  4:210643/114‑1 (MQ=255)
                                           tGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgc                                                                                                     <  2:1296859/149‑1 (MQ=255)
                                              cAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagc                                                                                                  >  3:1953/1‑149 (MQ=255)
                                               aaTGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGgcagca                                                                                                 >  3:349009/1‑149 (MQ=255)
                                                aTGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGc                                                                                                                                     >  4:527279/1‑112 (MQ=255)
                                                    cGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                            <  4:349009/149‑1 (MQ=255)
                                                    cGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                            <  1:1118588/149‑1 (MQ=255)
                                                          cAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGc                                                                                      <  3:66805/149‑1 (MQ=255)
                                                              ggAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAAtt                                                                                  <  3:1889205/149‑1 (MQ=255)
                                                                    gCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCg                                                                            <  4:815222/149‑1 (MQ=255)
                                                                      tCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCggg                                                                          >  1:1092691/1‑149 (MQ=255)
                                                                        cACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTc                                                                        <  4:1953/149‑1 (MQ=255)
                                                                         aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGGCATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTcc                                                                       <  4:1338366/149‑1 (MQ=255)
                                                                         aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTcc                                                                       >  3:829706/1‑149 (MQ=255)
                                                                          cGCCGTGGTCGGCGTAAACTGGTTTTCAAGGGTTGGTAATGGGTACATCTTTACCACATGTTCATCGCTATTAAACAGCGAACAGCCGCTTAATAGGGTAACGGAAAGCAGTCCTGGCATCAGTGATCTACGCAATTGCATCGGGTtcc                                                                      <  4:1177018/149‑1 (MQ=255)
                                                                           gCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCct                                                                     >  3:160578/1‑149 (MQ=255)
                                                                                  tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                              <  3:1401829/149‑1 (MQ=255)
                                                                                  tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGAt                                                              <  4:829706/149‑1 (MQ=255)
                                                                                   cGGCGTAAACTGTTTTTCAACGGTTGGCAATTGGGACATCTTTACCACATCTTCTTCTCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             <  2:1448368/149‑1 (MQ=255)
                                                                                     gCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGa                                                           <  3:1812665/149‑1 (MQ=255)
                                                                                       gTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAAt                                                                                            <  1:1522485/114‑1 (MQ=255)
                                                                                        tAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTcc                                                                       <  2:999004/134‑1 (MQ=255)
                                                                                          aaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGaa                                                                                                                                         >  1:914914/1‑66 (MQ=255)
                                                                                          aaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGaa                                                                                                                                         <  2:914914/66‑1 (MQ=255)
                                                                                           aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAAtt                                                                                  >  1:1421772/1‑120 (MQ=255)
                                                                                           aCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAatt                                                     >  4:594698/1‑149 (MQ=255)
                                                                                            cTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATc                                                                             >  2:1153554/1‑124 (MQ=255)
                                                                                             tGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                   >  2:525820/1‑149 (MQ=255)
                                                                                              ggTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattatt                                                  <  1:525820/149‑1 (MQ=255)
                                                                                               gTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAggg                                                                                                                         <  3:1522221/77‑1 (MQ=255)
                                                                                                    cAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAAtttt                                            >  1:1812220/1‑149 (MQ=255)
                                                                                                     aaCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAAt                                                                                   >  4:1377584/1‑109 (MQ=255)
                                                                                                       cGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             <  3:61540/129‑1 (MQ=255)
                                                                                                       cGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCAt                                         <  2:1540048/149‑1 (MQ=255)
                                                                                                          ttGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTaa                                                                                             >  4:1357578/1‑94 (MQ=255)
                                                                                                          ttGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTaa                                                                                             <  3:1357578/94‑1 (MQ=255)
                                                                                                             gCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcat                                   <  2:1812220/149‑1 (MQ=255)
                                                                                                                  ggggACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                   >  4:1534761/1‑128 (MQ=255)
                                                                                                                  ggggACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                   <  3:1534761/128‑1 (MQ=255)
                                                                                                                     gACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCa                                                                 <  2:854177/111‑1 (MQ=255)
                                                                                                                      aCATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCt                                                                                                        <  1:1360158/71‑1 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTa                                                                                         >  4:132784/1‑84 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTa                                                                                         <  3:132784/84‑1 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             <  2:764483/112‑1 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             >  1:764483/1‑112 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                   <  2:1451619/122‑1 (MQ=255)
                                                                                                                        aTCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAattat                                                   >  1:1451619/1‑122 (MQ=255)
                                                                                                                         tCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTc                                           <  1:1779481/129‑1 (MQ=255)
                                                                                                                         tCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTc                                           >  2:1779481/1‑129 (MQ=255)
                                                                                                                           tttACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                     >  4:1816935/1‑149 (MQ=255)
                                                                                                                           tttACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                     >  4:1695954/1‑149 (MQ=255)
                                                                                                                            ttACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGc                    <  3:594698/149‑1 (MQ=255)
                                                                                                                               ccACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCgg                 >  2:1070664/1‑149 (MQ=255)
                                                                                                                                 acaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCa                                                                               >  2:1276806/1‑85 (MQ=255)
                                                                                                                                 acaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCa                                                                               <  1:1276806/85‑1 (MQ=255)
                                                                                                                                 acaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATc                                                                             <  3:751361/87‑1 (MQ=255)
                                                                                                                                 acaTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATc                                                                             >  4:751361/1‑87 (MQ=255)
                                                                                                                                   aTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTa             >  1:616105/1‑149 (MQ=255)
                                                                                                                                    tcttctTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             <  4:1870839/100‑1 (MQ=255)
                                                                                                                                    tcttctTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATg                                                             >  3:1870839/1‑100 (MQ=255)
                                                                                                                                       tgttcGCTGTTTAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTa                                                                                         >  4:864755/3‑69 (MQ=255)
                                                                                                                                         ttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCgg                                                                           >  4:1414819/1‑81 (MQ=255)
                                                                                                                                         ttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCgg                                                                           <  3:926535/81‑1 (MQ=255)
                                                                                                                                         ttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGca                                    <  4:270390/120‑1 (MQ=255)
                                                                                                                                         ttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGca                                    >  3:270390/1‑120 (MQ=255)
                                                                                                                                          tcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGc      >  1:1023501/1‑149 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGaaa                                                                                                                <  1:19899/39‑1 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCtttt  <  4:120219/149‑1 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCtttt  >  4:689307/1‑149 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCtt    <  3:706843/147‑1 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCtt    >  4:706843/1‑147 (MQ=255)
                                                                                                                                              tGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCt     >  3:1723645/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTT  >  NC_000913/2638438‑2638728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: